Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes

The rock bream (Oplegnathus fasciatus) is a typical fish with a unique multiple sex chromosome system. In this study, we investigated the gene expression profiling in the gonads and brains of both males and females using RNA-Seq to identify sex-related genes and pathways. In accordance with the dimo...

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Autores principales:	Huan Li, Qihui Zhu, Ruiyi Chen, Mingtao Liu, Dongdong Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	gonadal development RNA-seq histological observation rock bream sex-related genes Genetics QH426-470
Acceso en línea:	https://doaj.org/article/14df475eb1224ec49bb1a733ecb0664b
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spelling	oai:doaj.org-article:14df475eb1224ec49bb1a733ecb0664b2021-12-01T13:43:32ZIdentification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes1664-802110.3389/fgene.2021.791179https://doaj.org/article/14df475eb1224ec49bb1a733ecb0664b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.791179/fullhttps://doaj.org/toc/1664-8021The rock bream (Oplegnathus fasciatus) is a typical fish with a unique multiple sex chromosome system. In this study, we investigated the gene expression profiling in the gonads and brains of both males and females using RNA-Seq to identify sex-related genes and pathways. In accordance with the dimorphic expression profiles, combined with Gene ontology and KEGG enrichment analyses, a number of potential genes and pathways associated with sex determination were obtained from transcriptional analysis, especially some sex-biased genes and pathways. Next, we selected 18 candidate genes and analyzed their expression in different tissues and developmental stages. We found that the expression levels of Amh, Dmrt1, Sox9, Dmrtb1, and Nanos2 were significantly higher in the testis than those in the ovary or other tissues, whereas the expression levels of ZP4, Bouncer, RNF208, FoxH1, and TOB were significantly higher in the ovary than those in the testis. Furthermore, the expression levels of these genes in different developmental stages of gonads also showed sexually dimorphic patterns, suggesting that they might play important roles during gonadal development. These genes are useful markers for investigating sex determination and differentiation in rock bream. The findings of this study can provide insights into the molecular mechanisms of sex determination and differentiation in fish with multiple sex chromosome systems.Huan LiHuan LiQihui ZhuQihui ZhuRuiyi ChenRuiyi ChenMingtao LiuMingtao LiuDongdong XuDongdong XuFrontiers Media S.A.articlegonadal developmentRNA-seqhistological observationrock breamsex-related genesGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gonadal development RNA-seq histological observation rock bream sex-related genes Genetics QH426-470
spellingShingle	gonadal development RNA-seq histological observation rock bream sex-related genes Genetics QH426-470 Huan Li Huan Li Qihui Zhu Qihui Zhu Ruiyi Chen Ruiyi Chen Mingtao Liu Mingtao Liu Dongdong Xu Dongdong Xu Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
description	The rock bream (Oplegnathus fasciatus) is a typical fish with a unique multiple sex chromosome system. In this study, we investigated the gene expression profiling in the gonads and brains of both males and females using RNA-Seq to identify sex-related genes and pathways. In accordance with the dimorphic expression profiles, combined with Gene ontology and KEGG enrichment analyses, a number of potential genes and pathways associated with sex determination were obtained from transcriptional analysis, especially some sex-biased genes and pathways. Next, we selected 18 candidate genes and analyzed their expression in different tissues and developmental stages. We found that the expression levels of Amh, Dmrt1, Sox9, Dmrtb1, and Nanos2 were significantly higher in the testis than those in the ovary or other tissues, whereas the expression levels of ZP4, Bouncer, RNF208, FoxH1, and TOB were significantly higher in the ovary than those in the testis. Furthermore, the expression levels of these genes in different developmental stages of gonads also showed sexually dimorphic patterns, suggesting that they might play important roles during gonadal development. These genes are useful markers for investigating sex determination and differentiation in rock bream. The findings of this study can provide insights into the molecular mechanisms of sex determination and differentiation in fish with multiple sex chromosome systems.
format	article
author	Huan Li Huan Li Qihui Zhu Qihui Zhu Ruiyi Chen Ruiyi Chen Mingtao Liu Mingtao Liu Dongdong Xu Dongdong Xu
author_facet	Huan Li Huan Li Qihui Zhu Qihui Zhu Ruiyi Chen Ruiyi Chen Mingtao Liu Mingtao Liu Dongdong Xu Dongdong Xu
author_sort	Huan Li
title	Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
title_short	Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
title_full	Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
title_fullStr	Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
title_full_unstemmed	Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes
title_sort	identification and characterization of dimorphic expression of sex-related genes in rock bream, a fish with multiple sex chromosomes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/14df475eb1224ec49bb1a733ecb0664b
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Identification and Characterization of Dimorphic Expression of Sex-Related Genes in Rock Bream, a Fish With Multiple Sex Chromosomes

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