NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency

Abstract Rice (Oryza sativa L.), a major dietary source, is often cultivated in soils poor in available inorganic orthophosphate (Pi), which is a key nutrient for growth and development. Poor soils are amended by phosphorus (P) fertilizer, which is derived from the non-renewable rock phosphate reser...

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Autores principales:	Yugandhar Poli, Veronica Nallamothu, Ai Hao, Muddapuram Deeksha Goud, Xiaowen Wang, Subrahmanyam Desiraju, Satendra K. Mangrauthia, Ajay Jain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14e0a0f2fba840eeab601dd9243af146
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spelling	oai:doaj.org-article:14e0a0f2fba840eeab601dd9243af1462021-12-02T16:55:45ZNH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency10.1038/s41598-021-88419-w2045-2322https://doaj.org/article/14e0a0f2fba840eeab601dd9243af1462021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88419-whttps://doaj.org/toc/2045-2322Abstract Rice (Oryza sativa L.), a major dietary source, is often cultivated in soils poor in available inorganic orthophosphate (Pi), which is a key nutrient for growth and development. Poor soils are amended by phosphorus (P) fertilizer, which is derived from the non-renewable rock phosphate reserves. Therefore, there is a need for developing rice varieties with high productivity under low P conditions. At the ICAR-IIRR, ethyl methanesulfonate (EMS) mutagenized rice genotype Nagina22 (N22) were screened for high grain yield in Pi-deprived soil, which led to the identification of ~ 10 gain-of-function mutants including NH787. Here, detailed comparative morphophysiological, biochemical, and molecular analyses of N22 and NH787 were carried out in hydroponics and potting soil under different Pi regimes. Under Pi-deprived condition, compared with N22, NH787 exhibited higher root and vegetative biomass, the number of tillers, and grain yield. The augmented agronomic traits of NH787 were corroborated with significantly higher photosynthetic rate, pollen fertility, stigma receptivity, and the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Further, several genes involved in the maintenance of Pi homeostasis (GPH) were differentially regulated. The study thus revealed a wide-spectrum influence of the mutation in NH787 that contributed towards its higher Pi use efficiency (PUE).Yugandhar PoliVeronica NallamothuAi HaoMuddapuram Deeksha GoudXiaowen WangSubrahmanyam DesirajuSatendra K. MangrauthiaAjay JainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yugandhar Poli Veronica Nallamothu Ai Hao Muddapuram Deeksha Goud Xiaowen Wang Subrahmanyam Desiraju Satendra K. Mangrauthia Ajay Jain NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
description	Abstract Rice (Oryza sativa L.), a major dietary source, is often cultivated in soils poor in available inorganic orthophosphate (Pi), which is a key nutrient for growth and development. Poor soils are amended by phosphorus (P) fertilizer, which is derived from the non-renewable rock phosphate reserves. Therefore, there is a need for developing rice varieties with high productivity under low P conditions. At the ICAR-IIRR, ethyl methanesulfonate (EMS) mutagenized rice genotype Nagina22 (N22) were screened for high grain yield in Pi-deprived soil, which led to the identification of ~ 10 gain-of-function mutants including NH787. Here, detailed comparative morphophysiological, biochemical, and molecular analyses of N22 and NH787 were carried out in hydroponics and potting soil under different Pi regimes. Under Pi-deprived condition, compared with N22, NH787 exhibited higher root and vegetative biomass, the number of tillers, and grain yield. The augmented agronomic traits of NH787 were corroborated with significantly higher photosynthetic rate, pollen fertility, stigma receptivity, and the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Further, several genes involved in the maintenance of Pi homeostasis (GPH) were differentially regulated. The study thus revealed a wide-spectrum influence of the mutation in NH787 that contributed towards its higher Pi use efficiency (PUE).
format	article
author	Yugandhar Poli Veronica Nallamothu Ai Hao Muddapuram Deeksha Goud Xiaowen Wang Subrahmanyam Desiraju Satendra K. Mangrauthia Ajay Jain
author_facet	Yugandhar Poli Veronica Nallamothu Ai Hao Muddapuram Deeksha Goud Xiaowen Wang Subrahmanyam Desiraju Satendra K. Mangrauthia Ajay Jain
author_sort	Yugandhar Poli
title	NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
title_short	NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
title_full	NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
title_fullStr	NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
title_full_unstemmed	NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency
title_sort	nh787 ems mutant of rice variety nagina22 exhibits higher phosphate use efficiency
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/14e0a0f2fba840eeab601dd9243af146
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NH787 EMS mutant of rice variety Nagina22 exhibits higher phosphate use efficiency

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