Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens

In recent years, pathogenic strains of Enterococcus cecorum (EC) have emerged as a causing agent of septicemia and skeletal infection in broiler chickens with a high economic impact worldwide. Although research has been conducted, many aspects of the pathogenesis of the EC-associated disease are sti...

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Autores principales:	Jana Schreier, Silke Rautenschlein, Arne Jung
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14eed2eac8c34e6695689df631ee3646
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spelling	oai:doaj.org-article:14eed2eac8c34e6695689df631ee36462021-11-25T06:11:03ZDifferent virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens1932-6203https://doaj.org/article/14eed2eac8c34e6695689df631ee36462021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589174/?tool=EBIhttps://doaj.org/toc/1932-6203In recent years, pathogenic strains of Enterococcus cecorum (EC) have emerged as a causing agent of septicemia and skeletal infection in broiler chickens with a high economic impact worldwide. Although research has been conducted, many aspects of the pathogenesis of the EC-associated disease are still unknown. In the present study, an experimental infection model was established in broiler chickens. Two different EC strains (EC14 and EC15) were compared in two different concentrations of each strain (2 × 106 and 2 × 108 colony-forming units per milliliter (CFU/mL)) after oral infection of one-day-old chicks. Clinical signs and gross lesions of the EC-associated disease were monitored in the following seven weeks. Although both EC strains were originally isolated from clinical disease outbreaks and had a high embryonic lethality, only EC14 successfully induced the typical course of the EC-associated disease with characteristic clinical signs and gross lesions. In total, 23% of the birds in the two EC14-groups were EC-positive in extraintestinal organs on culture, and no differences were found between the two infectious doses. EC14 was frequently detected via real-time PCR in the free thoracic vertebra (FTV) and femoral heads without any detectable gross lesions. The number of EC positive spleens from infected broilers was comparable using bacterial isolation and a specific real-time PCR. Interestingly, EC15 was not detected in extraintestinal organs, although birds in the EC15 groups were colonized by EC in the ceca after experimental infection. The present study represents first proof that virulence differs among EC strains in experimentally infected chickens, and emphasizes the need to further characterize virulence factors and pathogenic mechanisms of EC. The strain EC14 at a dose of 106 CFU is suitable for reproduction of the EC-associated disease. The experimental infection model reported here provides the basis for further research on the EC pathogenesis and possible prevention and intervention strategies.Jana SchreierSilke RautenschleinArne JungPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jana Schreier Silke Rautenschlein Arne Jung Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
description	In recent years, pathogenic strains of Enterococcus cecorum (EC) have emerged as a causing agent of septicemia and skeletal infection in broiler chickens with a high economic impact worldwide. Although research has been conducted, many aspects of the pathogenesis of the EC-associated disease are still unknown. In the present study, an experimental infection model was established in broiler chickens. Two different EC strains (EC14 and EC15) were compared in two different concentrations of each strain (2 × 106 and 2 × 108 colony-forming units per milliliter (CFU/mL)) after oral infection of one-day-old chicks. Clinical signs and gross lesions of the EC-associated disease were monitored in the following seven weeks. Although both EC strains were originally isolated from clinical disease outbreaks and had a high embryonic lethality, only EC14 successfully induced the typical course of the EC-associated disease with characteristic clinical signs and gross lesions. In total, 23% of the birds in the two EC14-groups were EC-positive in extraintestinal organs on culture, and no differences were found between the two infectious doses. EC14 was frequently detected via real-time PCR in the free thoracic vertebra (FTV) and femoral heads without any detectable gross lesions. The number of EC positive spleens from infected broilers was comparable using bacterial isolation and a specific real-time PCR. Interestingly, EC15 was not detected in extraintestinal organs, although birds in the EC15 groups were colonized by EC in the ceca after experimental infection. The present study represents first proof that virulence differs among EC strains in experimentally infected chickens, and emphasizes the need to further characterize virulence factors and pathogenic mechanisms of EC. The strain EC14 at a dose of 106 CFU is suitable for reproduction of the EC-associated disease. The experimental infection model reported here provides the basis for further research on the EC pathogenesis and possible prevention and intervention strategies.
format	article
author	Jana Schreier Silke Rautenschlein Arne Jung
author_facet	Jana Schreier Silke Rautenschlein Arne Jung
author_sort	Jana Schreier
title	Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
title_short	Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
title_full	Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
title_fullStr	Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
title_full_unstemmed	Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens
title_sort	different virulence levels of enterococcus cecorum strains in experimentally infected meat-type chickens
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/14eed2eac8c34e6695689df631ee3646
work_keys_str_mv	AT janaschreier differentvirulencelevelsofenterococcuscecorumstrainsinexperimentallyinfectedmeattypechickens AT silkerautenschlein differentvirulencelevelsofenterococcuscecorumstrainsinexperimentallyinfectedmeattypechickens AT arnejung differentvirulencelevelsofenterococcuscecorumstrainsinexperimentallyinfectedmeattypechickens
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Different virulence levels of Enterococcus cecorum strains in experimentally infected meat-type chickens

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