Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.

Hepatitis B virus (HBV) produces high quantities of subviral surface antigen particles (HBsAg) which circulate in the blood outnumbering virions of about 1\10(3-6) times. In individuals coinfected with the defective hepatitis Delta virus (HDV) the small HDV-RNA-genome and Delta antigen circulate as...

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Autores principales:	Luisa Novellino, Riccardo L Rossi, Ferruccio Bonino, Daniela Cavallone, Sergio Abrignani, Massimiliano Pagani, Maurizia R Brunetto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/14fa10377ddc472aab5f610fc0dd89f2
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spelling	oai:doaj.org-article:14fa10377ddc472aab5f610fc0dd89f22021-11-18T07:24:09ZCirculating hepatitis B surface antigen particles carry hepatocellular microRNAs.1932-620310.1371/journal.pone.0031952https://doaj.org/article/14fa10377ddc472aab5f610fc0dd89f22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22470417/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hepatitis B virus (HBV) produces high quantities of subviral surface antigen particles (HBsAg) which circulate in the blood outnumbering virions of about 1\10(3-6) times. In individuals coinfected with the defective hepatitis Delta virus (HDV) the small HDV-RNA-genome and Delta antigen circulate as ribonucleoprotein complexes within HBsAg subviral particles. We addressed the question whether subviral HBsAg particles may carry in the same way cellular microRNAs (miRNAs) which are released into the bloodstream within different subcellular forms such as exosomes and microvescicles. Circulating HBsAg particles were isolated from sera of 11 HBsAg carriers by selective immunoprecipitation with monoclonal anti-HBs-IgG, total RNA was extracted and human miRNAs were screened by TaqMan real-time quantitative PCR Arrays. Thirty-nine human miRNAs were found to be significantly associated with the immunoprecipitated HBsAg, as determined by both comparative DDCT analysis and non-parametric tests (Mann-Whitney, p<0.05) with respect to controls. Moreover immunoprecipitated HBsAg particles contained Ago2 protein that could be revealed in ELISA only after 0.5% NP40. HBsAg associated miRNAs were liver-specific (most frequent = miR-27a, miR-30b, miR-122, miR-126 and miR-145) as well as immune regulatory (most frequent = miR-106b and miR-223). Computationally predicted target genes of HBsAg-associated miRNAs highlighted molecular pathways dealing with host-pathogen. The finding that HBsAg particles carry selective pools of hepatocellular miRNAs opens new avenues of research to disentangle the complex interactions between host and HBV and provides a non invasive tool to study the physiopathology of liver epigenetics.Luisa NovellinoRiccardo L RossiFerruccio BoninoDaniela CavalloneSergio AbrignaniMassimiliano PaganiMaurizia R BrunettoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e31952 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luisa Novellino Riccardo L Rossi Ferruccio Bonino Daniela Cavallone Sergio Abrignani Massimiliano Pagani Maurizia R Brunetto Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
description	Hepatitis B virus (HBV) produces high quantities of subviral surface antigen particles (HBsAg) which circulate in the blood outnumbering virions of about 1\10(3-6) times. In individuals coinfected with the defective hepatitis Delta virus (HDV) the small HDV-RNA-genome and Delta antigen circulate as ribonucleoprotein complexes within HBsAg subviral particles. We addressed the question whether subviral HBsAg particles may carry in the same way cellular microRNAs (miRNAs) which are released into the bloodstream within different subcellular forms such as exosomes and microvescicles. Circulating HBsAg particles were isolated from sera of 11 HBsAg carriers by selective immunoprecipitation with monoclonal anti-HBs-IgG, total RNA was extracted and human miRNAs were screened by TaqMan real-time quantitative PCR Arrays. Thirty-nine human miRNAs were found to be significantly associated with the immunoprecipitated HBsAg, as determined by both comparative DDCT analysis and non-parametric tests (Mann-Whitney, p<0.05) with respect to controls. Moreover immunoprecipitated HBsAg particles contained Ago2 protein that could be revealed in ELISA only after 0.5% NP40. HBsAg associated miRNAs were liver-specific (most frequent = miR-27a, miR-30b, miR-122, miR-126 and miR-145) as well as immune regulatory (most frequent = miR-106b and miR-223). Computationally predicted target genes of HBsAg-associated miRNAs highlighted molecular pathways dealing with host-pathogen. The finding that HBsAg particles carry selective pools of hepatocellular miRNAs opens new avenues of research to disentangle the complex interactions between host and HBV and provides a non invasive tool to study the physiopathology of liver epigenetics.
format	article
author	Luisa Novellino Riccardo L Rossi Ferruccio Bonino Daniela Cavallone Sergio Abrignani Massimiliano Pagani Maurizia R Brunetto
author_facet	Luisa Novellino Riccardo L Rossi Ferruccio Bonino Daniela Cavallone Sergio Abrignani Massimiliano Pagani Maurizia R Brunetto
author_sort	Luisa Novellino
title	Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
title_short	Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
title_full	Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
title_fullStr	Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
title_full_unstemmed	Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
title_sort	circulating hepatitis b surface antigen particles carry hepatocellular micrornas.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/14fa10377ddc472aab5f610fc0dd89f2
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Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.

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