The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions

Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetof...

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Autores principales:	Lucia Balejčíková, Karel Saksl, Jozef Kováč, Anne Martel, Vasil M. Garamus, Mikhail V. Avdeev, Viktor I. Petrenko, László Almásy, Peter Kopčanský
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	magnetoferritin magnetite loading factor protein stability aqueous medium Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/14fd86fc1fa74d149209f302da826dc5
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spelling	oai:doaj.org-article:14fd86fc1fa74d149209f302da826dc52021-11-25T18:28:46ZThe Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions10.3390/molecules262269601420-3049https://doaj.org/article/14fd86fc1fa74d149209f302da826dc52021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6960https://doaj.org/toc/1420-3049Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite.Lucia BalejčíkováKarel SakslJozef KováčAnne MartelVasil M. GaramusMikhail V. AvdeevViktor I. PetrenkoLászló AlmásyPeter KopčanskýMDPI AGarticlemagnetoferritinmagnetiteloading factorprotein stabilityaqueous mediumOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6960, p 6960 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetoferritin magnetite loading factor protein stability aqueous medium Organic chemistry QD241-441
spellingShingle	magnetoferritin magnetite loading factor protein stability aqueous medium Organic chemistry QD241-441 Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
description	Ferritin, a spherically shaped protein complex, is responsible for iron storage in bacteria, plants, animals, and humans. Various ferritin iron core compositions in organisms are associated with specific living requirements, health state, and different biochemical roles of ferritin isomers. Magnetoferritin, a synthetic ferritin derivative, serves as an artificial model system of unusual iron phase structures found in humans. We present the results of a complex structural study of magnetoferritins prepared by controlled in vitro synthesis. Using various complementary methods, it was observed that manipulation of the synthesis technology can improve the physicochemical parameters of the system, which is useful in applications. Thus, a higher synthesis temperature leads to an increase in magnetization due to the formation of the magnetite phase. An increase in the iron loading factor has a more pronounced impact on the protein shell structure in comparison with the pH of the aqueous medium. On the other hand, a higher loading factor at physiological temperature enhances the formation of an amorphous phase instead of magnetite crystallization. It was confirmed that the iron-overloading effect alone (observed during pathological events) cannot contribute to the formation of magnetite.
format	article
author	Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský
author_facet	Lucia Balejčíková Karel Saksl Jozef Kováč Anne Martel Vasil M. Garamus Mikhail V. Avdeev Viktor I. Petrenko László Almásy Peter Kopčanský
author_sort	Lucia Balejčíková
title	The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
title_short	The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
title_full	The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
title_fullStr	The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
title_full_unstemmed	The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions
title_sort	impact of redox, hydrolysis and dehydration chemistry on the structural and magnetic properties of magnetoferritin prepared in variable thermal conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/14fd86fc1fa74d149209f302da826dc5
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The Impact of Redox, Hydrolysis and Dehydration Chemistry on the Structural and Magnetic Properties of Magnetoferritin Prepared in Variable Thermal Conditions

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