Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues

Collagen has grown increasingly present in bioprinting, however collagen bioprinting has mostly been limited to the extrusion printing of collagen type I to form weak collagen hydrogels. While these weak collagen hydrogels have their applications, synthetic polymers are often required to reinforce g...

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Autores principales:	Rory Gibney, Eleonora Ferraris
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioprinting 3D printing biomaterials connective tissue collagen type II collagen type I Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1501e509e54e4555a59ef06ed638c5d1
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spelling	oai:doaj.org-article:1501e509e54e4555a59ef06ed638c5d12021-11-05T06:49:52ZBioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues2296-418510.3389/fbioe.2021.786945https://doaj.org/article/1501e509e54e4555a59ef06ed638c5d12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.786945/fullhttps://doaj.org/toc/2296-4185Collagen has grown increasingly present in bioprinting, however collagen bioprinting has mostly been limited to the extrusion printing of collagen type I to form weak collagen hydrogels. While these weak collagen hydrogels have their applications, synthetic polymers are often required to reinforce gel-laden constructs that aim to replicate dense collagenous tissues found in vivo. In this study, aerosol jet printing (AJP) was used to print and process collagen type I and II into dense constructs with a greater capacity to replicate the dense collagenous ECM found in connective tissues. Collagen type I and II was isolated from animal tissues to form solutions for printing. Collagen type I and II constructs were printed with 576 layers and measured to have average effective elastic moduli of 241.3 ± 94.3 and 196.6 ± 86.0 kPa (±SD), respectively, without any chemical modification. Collagen type II solutions were measured to be less viscous than type I and both collagen type I and II exhibited a drop in viscosity due to AJP. Circular dichroism and SDS-PAGE showed collagen type I to be more vulnerable to structural changes due to the stresses of the aerosol formation step of aerosol jet printing while the collagen type II triple helix was largely unaffected. SEM illustrated that distinct layers remained in the aerosol jet print constructs. The results show that aerosol jet printing should be considered an effective way to process collagen type I and II into stiff dense constructs with suitable mechanical properties for the replication of dense collagenous connective tissues.Rory GibneyRory GibneyEleonora FerrarisFrontiers Media S.A.articlebioprinting3D printingbiomaterialsconnective tissuecollagen type IIcollagen type IBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioprinting 3D printing biomaterials connective tissue collagen type II collagen type I Biotechnology TP248.13-248.65
spellingShingle	bioprinting 3D printing biomaterials connective tissue collagen type II collagen type I Biotechnology TP248.13-248.65 Rory Gibney Rory Gibney Eleonora Ferraris Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
description	Collagen has grown increasingly present in bioprinting, however collagen bioprinting has mostly been limited to the extrusion printing of collagen type I to form weak collagen hydrogels. While these weak collagen hydrogels have their applications, synthetic polymers are often required to reinforce gel-laden constructs that aim to replicate dense collagenous tissues found in vivo. In this study, aerosol jet printing (AJP) was used to print and process collagen type I and II into dense constructs with a greater capacity to replicate the dense collagenous ECM found in connective tissues. Collagen type I and II was isolated from animal tissues to form solutions for printing. Collagen type I and II constructs were printed with 576 layers and measured to have average effective elastic moduli of 241.3 ± 94.3 and 196.6 ± 86.0 kPa (±SD), respectively, without any chemical modification. Collagen type II solutions were measured to be less viscous than type I and both collagen type I and II exhibited a drop in viscosity due to AJP. Circular dichroism and SDS-PAGE showed collagen type I to be more vulnerable to structural changes due to the stresses of the aerosol formation step of aerosol jet printing while the collagen type II triple helix was largely unaffected. SEM illustrated that distinct layers remained in the aerosol jet print constructs. The results show that aerosol jet printing should be considered an effective way to process collagen type I and II into stiff dense constructs with suitable mechanical properties for the replication of dense collagenous connective tissues.
format	article
author	Rory Gibney Rory Gibney Eleonora Ferraris
author_facet	Rory Gibney Rory Gibney Eleonora Ferraris
author_sort	Rory Gibney
title	Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
title_short	Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
title_full	Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
title_fullStr	Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
title_full_unstemmed	Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues
title_sort	bioprinting of collagen type i and ii via aerosol jet printing for the replication of dense collagenous tissues
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1501e509e54e4555a59ef06ed638c5d1
work_keys_str_mv	AT rorygibney bioprintingofcollagentypeiandiiviaaerosoljetprintingforthereplicationofdensecollagenoustissues AT rorygibney bioprintingofcollagentypeiandiiviaaerosoljetprintingforthereplicationofdensecollagenoustissues AT eleonoraferraris bioprintingofcollagentypeiandiiviaaerosoljetprintingforthereplicationofdensecollagenoustissues
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Bioprinting of Collagen Type I and II via Aerosol Jet Printing for the Replication of Dense Collagenous Tissues

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