Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (Laminaria japonica Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway

The purpose of the present study was to explore the efficacy of fermented extract of sea tangle (Laminaria japonica Aresch, FST) with Lactobacillus brevis on DNA damage and apoptosis in hydrogen peroxide (H2O2)-stimu...

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Autores principales:	So Young Kim, Hee-Jae Cha, Hyun Hwangbo, Cheol Park, Hyesook Lee, Kyoung Seob Song, Jung-Hyun Shim, Jeong Sook Noh, Heui-Soo Kim, Bae-Jin Lee, Suhkmann Kim, Gi-Young Kim, You-Jin Jeon, Yung Hyun Choi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fermented sea tangle osteoblast ROS apoptosis Nrf2/HO-1 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/1503b3e472314af1b6ca09109f9a3019
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spelling	oai:doaj.org-article:1503b3e472314af1b6ca09109f9a30192021-11-25T17:35:51ZProtection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway10.3390/foods101128072304-8158https://doaj.org/article/1503b3e472314af1b6ca09109f9a30192021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2807https://doaj.org/toc/2304-8158The purpose of the present study was to explore the efficacy of fermented extract of sea tangle (<i>Laminaria japonica</i> Aresch, FST) with <i>Lactobacillus brevis</i> on DNA damage and apoptosis in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-stimulated osteoblastic MC3T3-E1 cells and clarify related signaling pathways. Our results showed that exposure to FST significantly improved cell viability, inhibited apoptosis, and suppressed the generation of reactive oxygen species (ROS) in H<sub>2</sub>O<sub>2</sub>-stimulated cells. In addition, H<sub>2</sub>O<sub>2</sub> triggered DNA damage in MC3T3-E1 cells was markedly attenuated by FST pretreatment. Moreover, H<sub>2</sub>O<sub>2</sub>-induced mitochondrial dysfunctions associated with apoptotic events, including loss of mitochondrial membrane potential (MMP), decreased Bcl-2/Bcl-2 associated x-protein (Bax) ratio, and cytosolic release of cytochrome <i>c</i>, were reduced in the presence of FST. FST also diminished H<sub>2</sub>O<sub>2</sub>-induced activation of caspase-3, which was associated with the ability of FST to protect the degradation of poly (ADP-ribose) polymerase. Furthermore, FST notably enhanced nuclear translocation and phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) in the presence of H<sub>2</sub>O<sub>2</sub> with concomitant upregulation of heme oxygenase-1 (HO-1) expression. However, artificial blockade of this pathway by the HO-1 inhibitor, zinc protoporphyrin IX, greatly abolished the protective effect of FST against H<sub>2</sub>O<sub>2</sub>-induced MC3T3-E1 cell injury. Taken together, these results demonstrate that FST could protect MC3T3-E1 cells from H<sub>2</sub>O<sub>2</sub>-induced damage by maintaining mitochondrial function while eliminating ROS along with activation of the Nrf2/HO-1 antioxidant pathway.So Young KimHee-Jae ChaHyun HwangboCheol ParkHyesook LeeKyoung Seob SongJung-Hyun ShimJeong Sook NohHeui-Soo KimBae-Jin LeeSuhkmann KimGi-Young KimYou-Jin JeonYung Hyun ChoiMDPI AGarticlefermented sea tangleosteoblastROSapoptosisNrf2/HO-1Chemical technologyTP1-1185ENFoods, Vol 10, Iss 2807, p 2807 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fermented sea tangle osteoblast ROS apoptosis Nrf2/HO-1 Chemical technology TP1-1185
spellingShingle	fermented sea tangle osteoblast ROS apoptosis Nrf2/HO-1 Chemical technology TP1-1185 So Young Kim Hee-Jae Cha Hyun Hwangbo Cheol Park Hyesook Lee Kyoung Seob Song Jung-Hyun Shim Jeong Sook Noh Heui-Soo Kim Bae-Jin Lee Suhkmann Kim Gi-Young Kim You-Jin Jeon Yung Hyun Choi Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
description	The purpose of the present study was to explore the efficacy of fermented extract of sea tangle (<i>Laminaria japonica</i> Aresch, FST) with <i>Lactobacillus brevis</i> on DNA damage and apoptosis in hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-stimulated osteoblastic MC3T3-E1 cells and clarify related signaling pathways. Our results showed that exposure to FST significantly improved cell viability, inhibited apoptosis, and suppressed the generation of reactive oxygen species (ROS) in H<sub>2</sub>O<sub>2</sub>-stimulated cells. In addition, H<sub>2</sub>O<sub>2</sub> triggered DNA damage in MC3T3-E1 cells was markedly attenuated by FST pretreatment. Moreover, H<sub>2</sub>O<sub>2</sub>-induced mitochondrial dysfunctions associated with apoptotic events, including loss of mitochondrial membrane potential (MMP), decreased Bcl-2/Bcl-2 associated x-protein (Bax) ratio, and cytosolic release of cytochrome <i>c</i>, were reduced in the presence of FST. FST also diminished H<sub>2</sub>O<sub>2</sub>-induced activation of caspase-3, which was associated with the ability of FST to protect the degradation of poly (ADP-ribose) polymerase. Furthermore, FST notably enhanced nuclear translocation and phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) in the presence of H<sub>2</sub>O<sub>2</sub> with concomitant upregulation of heme oxygenase-1 (HO-1) expression. However, artificial blockade of this pathway by the HO-1 inhibitor, zinc protoporphyrin IX, greatly abolished the protective effect of FST against H<sub>2</sub>O<sub>2</sub>-induced MC3T3-E1 cell injury. Taken together, these results demonstrate that FST could protect MC3T3-E1 cells from H<sub>2</sub>O<sub>2</sub>-induced damage by maintaining mitochondrial function while eliminating ROS along with activation of the Nrf2/HO-1 antioxidant pathway.
format	article
author	So Young Kim Hee-Jae Cha Hyun Hwangbo Cheol Park Hyesook Lee Kyoung Seob Song Jung-Hyun Shim Jeong Sook Noh Heui-Soo Kim Bae-Jin Lee Suhkmann Kim Gi-Young Kim You-Jin Jeon Yung Hyun Choi
author_facet	So Young Kim Hee-Jae Cha Hyun Hwangbo Cheol Park Hyesook Lee Kyoung Seob Song Jung-Hyun Shim Jeong Sook Noh Heui-Soo Kim Bae-Jin Lee Suhkmann Kim Gi-Young Kim You-Jin Jeon Yung Hyun Choi
author_sort	So Young Kim
title	Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
title_short	Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
title_full	Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
title_fullStr	Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
title_full_unstemmed	Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway
title_sort	protection against oxidative stress-induced apoptosis by fermented sea tangle (<i>laminaria japonica</i> aresch) in osteoblastic mc3t3-e1 cells through activation of nrf2 signaling pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1503b3e472314af1b6ca09109f9a3019
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Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (<i>Laminaria japonica</i> Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway

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