Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?

Endometrosis is an important mares’ disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffman...

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Autores principales:	Tomasz Jasiński, Łukasz Zdrojkowski, Ewa Kautz, Edyta Juszczuk-Kubiak, Graça Ferreira-Dias, Małgorzata Domino
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometrosis mare NF-κB MCP-1 IL-6 HAS Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/15079ee74aed4990a5dbb1b7ae91593c
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spelling	oai:doaj.org-article:15079ee74aed4990a5dbb1b7ae91593c2021-11-25T16:17:10ZEquine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?10.3390/ani111131512076-2615https://doaj.org/article/15079ee74aed4990a5dbb1b7ae91593c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3151https://doaj.org/toc/2076-2615Endometrosis is an important mares’ disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffmann and others. This study aimed to compare NF-κB pathway genes transcription among histopathological types of endometrosis, following Hoffmann and co-authors’ classification. Endometrial samples, collected postmortem from cyclic mares (<i>n</i> = 100) in estrus or diestrus, were classified histologically and used for gene transcription assessment. Gene transcription of NF-κB subunits (<i>RelA</i>, <i>NF-κB1</i>, <i>NF-κB2</i>), pro-inflammatory molecules (<i>MCP-1</i>, <i>IL-6</i>), and hyaluronan synthases (<i>HAS 1</i>, <i>HAS 2</i>, <i>HAS 3</i>) was compared among endometrosis types (active, non-active, destructive, non-destructive). Most individual mRNA samples showed high expression of <i>RelA</i>, <i>NF-κB1</i>, and <i>MCP-1</i> gene transcripts and the destructive type of endometrosis, simultaneously. The expression of <i>RelA</i> and <i>NF-κB1</i> genes was higher in active destructive group than in the other groups only in the follicular phase, as well as being higher in the inactive destructive group than in the others, only in the mid-luteal phase. The increase in gene transcription of the NF-κB canonical activation pathway in destructive endometrosis may suggest the highest changes in extracellular matrix deposition. Moreover, the estrous cycle phase might influence fibrosis pathogenesis.Tomasz JasińskiŁukasz ZdrojkowskiEwa KautzEdyta Juszczuk-KubiakGraça Ferreira-DiasMałgorzata DominoMDPI AGarticleendometrosismareNF-κBMCP-1IL-6HASVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3151, p 3151 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometrosis mare NF-κB MCP-1 IL-6 HAS Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	endometrosis mare NF-κB MCP-1 IL-6 HAS Veterinary medicine SF600-1100 Zoology QL1-991 Tomasz Jasiński Łukasz Zdrojkowski Ewa Kautz Edyta Juszczuk-Kubiak Graça Ferreira-Dias Małgorzata Domino Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
description	Endometrosis is an important mares’ disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffmann and others. This study aimed to compare NF-κB pathway genes transcription among histopathological types of endometrosis, following Hoffmann and co-authors’ classification. Endometrial samples, collected postmortem from cyclic mares (<i>n</i> = 100) in estrus or diestrus, were classified histologically and used for gene transcription assessment. Gene transcription of NF-κB subunits (<i>RelA</i>, <i>NF-κB1</i>, <i>NF-κB2</i>), pro-inflammatory molecules (<i>MCP-1</i>, <i>IL-6</i>), and hyaluronan synthases (<i>HAS 1</i>, <i>HAS 2</i>, <i>HAS 3</i>) was compared among endometrosis types (active, non-active, destructive, non-destructive). Most individual mRNA samples showed high expression of <i>RelA</i>, <i>NF-κB1</i>, and <i>MCP-1</i> gene transcripts and the destructive type of endometrosis, simultaneously. The expression of <i>RelA</i> and <i>NF-κB1</i> genes was higher in active destructive group than in the other groups only in the follicular phase, as well as being higher in the inactive destructive group than in the others, only in the mid-luteal phase. The increase in gene transcription of the NF-κB canonical activation pathway in destructive endometrosis may suggest the highest changes in extracellular matrix deposition. Moreover, the estrous cycle phase might influence fibrosis pathogenesis.
format	article
author	Tomasz Jasiński Łukasz Zdrojkowski Ewa Kautz Edyta Juszczuk-Kubiak Graça Ferreira-Dias Małgorzata Domino
author_facet	Tomasz Jasiński Łukasz Zdrojkowski Ewa Kautz Edyta Juszczuk-Kubiak Graça Ferreira-Dias Małgorzata Domino
author_sort	Tomasz Jasiński
title	Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
title_short	Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
title_full	Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
title_fullStr	Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
title_full_unstemmed	Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?
title_sort	equine endometrosis pathological features: are they dependent on nf-κb signaling pathway?
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/15079ee74aed4990a5dbb1b7ae91593c
work_keys_str_mv	AT tomaszjasinski equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway AT łukaszzdrojkowski equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway AT ewakautz equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway AT edytajuszczukkubiak equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway AT gracaferreiradias equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway AT małgorzatadomino equineendometrosispathologicalfeaturesaretheydependentonnfkbsignalingpathway
_version_	1718413240320393216

Equine Endometrosis Pathological Features: Are They Dependent on NF-κB Signaling Pathway?

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