A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake

Abstract Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daxing Wen, Haicheng Xu, Liuyong Xie, Mingrong He, Hongcun Hou, Chunqing Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/150a6c6411d84828baa0b4b8f74cf3ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:150a6c6411d84828baa0b4b8f74cf3ce
record_format	dspace
spelling	oai:doaj.org-article:150a6c6411d84828baa0b4b8f74cf3ce2021-12-02T15:05:47ZA loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake10.1038/s41598-017-03333-42045-2322https://doaj.org/article/150a6c6411d84828baa0b4b8f74cf3ce2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03333-4https://doaj.org/toc/2045-2322Abstract Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was to study the mechanism of rapid seed germination and explore which pathways and genes play critical roles in radicle protrusion. Anatomical data revealed that seed protein content affected endosperm structure of seeds. Moreover, scanning electron microscope maps showed that faster germinated seeds had a looser endosperm structure compared with other seeds. Subsequently, high throughout RNA-seq data were used to compare the transcriptomes of imbibed seeds with different germination speed. Gene ontology (GO) term enrichment analysis revealed that cell wall metabolism related genes significantly up-regulated in faster germinated seeds. In these genes, the top four were chitinase that had about fourfold higher expression in faster germinated seeds. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that faster germinated seeds had enhanced expression in glutathione metabolism. By combining these results, we propose a model for nitrogen fertilizer affects germination speed of wheat seed, which provide new insights into seed germination.Daxing WenHaicheng XuLiuyong XieMingrong HeHongcun HouChunqing ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daxing Wen Haicheng Xu Liuyong Xie Mingrong He Hongcun Hou Chunqing Zhang A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
description	Abstract Water uptake is the fundamental requirement for the initiation and completion of seed germination that is a vital phase in the life cycle of seed plants. We found that seeds produced under four nitrogen levels showed significantly different germination speed. The objective of this study was to study the mechanism of rapid seed germination and explore which pathways and genes play critical roles in radicle protrusion. Anatomical data revealed that seed protein content affected endosperm structure of seeds. Moreover, scanning electron microscope maps showed that faster germinated seeds had a looser endosperm structure compared with other seeds. Subsequently, high throughout RNA-seq data were used to compare the transcriptomes of imbibed seeds with different germination speed. Gene ontology (GO) term enrichment analysis revealed that cell wall metabolism related genes significantly up-regulated in faster germinated seeds. In these genes, the top four were chitinase that had about fourfold higher expression in faster germinated seeds. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that faster germinated seeds had enhanced expression in glutathione metabolism. By combining these results, we propose a model for nitrogen fertilizer affects germination speed of wheat seed, which provide new insights into seed germination.
format	article
author	Daxing Wen Haicheng Xu Liuyong Xie Mingrong He Hongcun Hou Chunqing Zhang
author_facet	Daxing Wen Haicheng Xu Liuyong Xie Mingrong He Hongcun Hou Chunqing Zhang
author_sort	Daxing Wen
title	A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
title_short	A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
title_full	A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
title_fullStr	A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
title_full_unstemmed	A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
title_sort	loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/150a6c6411d84828baa0b4b8f74cf3ce
work_keys_str_mv	AT daxingwen alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT haichengxu alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT liuyongxie alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT mingronghe alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT hongcunhou alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT chunqingzhang alooseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT daxingwen looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT haichengxu looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT liuyongxie looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT mingronghe looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT hongcunhou looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake AT chunqingzhang looseendospermstructureofwheatseedproducedunderlownitrogenlevelpromotesearlygerminationbyacceleratingwateruptake
_version_	1718388689963319296

A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake

Ejemplares similares