Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Abstract Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing un...

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Autores principales:	Viviane A. Guilherme, Lígia N. M. Ribeiro, Ana C. S. Alcântara, Simone R. Castro, Gustavo H. Rodrigues da Silva, Camila Gonçalves da Silva, Márcia C. Breitkreitz, Juliana Clemente-Napimoga, Cristina G. Macedo, Henrique B. Abdalla, Ricardo Bonfante, Cintia M. S. Cereda, Eneida de Paula
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15100612c45847f0a2173cee1472093a
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spelling	oai:doaj.org-article:15100612c45847f0a2173cee1472093a2021-12-02T15:08:46ZImproved efficacy of naproxen-loaded NLC for temporomandibular joint administration10.1038/s41598-019-47486-w2045-2322https://doaj.org/article/15100612c45847f0a2173cee1472093a2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47486-whttps://doaj.org/toc/2045-2322Abstract Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (23) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 °C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1β and TNF-α), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints.Viviane A. GuilhermeLígia N. M. RibeiroAna C. S. AlcântaraSimone R. CastroGustavo H. Rodrigues da SilvaCamila Gonçalves da SilvaMárcia C. BreitkreitzJuliana Clemente-NapimogaCristina G. MacedoHenrique B. AbdallaRicardo BonfanteCintia M. S. CeredaEneida de PaulaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viviane A. Guilherme Lígia N. M. Ribeiro Ana C. S. Alcântara Simone R. Castro Gustavo H. Rodrigues da Silva Camila Gonçalves da Silva Márcia C. Breitkreitz Juliana Clemente-Napimoga Cristina G. Macedo Henrique B. Abdalla Ricardo Bonfante Cintia M. S. Cereda Eneida de Paula Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
description	Abstract Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (23) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 °C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1β and TNF-α), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints.
format	article
author	Viviane A. Guilherme Lígia N. M. Ribeiro Ana C. S. Alcântara Simone R. Castro Gustavo H. Rodrigues da Silva Camila Gonçalves da Silva Márcia C. Breitkreitz Juliana Clemente-Napimoga Cristina G. Macedo Henrique B. Abdalla Ricardo Bonfante Cintia M. S. Cereda Eneida de Paula
author_facet	Viviane A. Guilherme Lígia N. M. Ribeiro Ana C. S. Alcântara Simone R. Castro Gustavo H. Rodrigues da Silva Camila Gonçalves da Silva Márcia C. Breitkreitz Juliana Clemente-Napimoga Cristina G. Macedo Henrique B. Abdalla Ricardo Bonfante Cintia M. S. Cereda Eneida de Paula
author_sort	Viviane A. Guilherme
title	Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
title_short	Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
title_full	Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
title_fullStr	Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
title_full_unstemmed	Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration
title_sort	improved efficacy of naproxen-loaded nlc for temporomandibular joint administration
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/15100612c45847f0a2173cee1472093a
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Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

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