Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.

Retinoblastoma binding protein 6 (RBBP6) plays an important role in chaperone-mediated ubiquitination and interacts with TP53 in carcinogenesis. However, the clinicopathologic significance of RBBP6 expression in colon cancer is unknown; in particular, the prognostic value of RBBP6 combined with TP53...

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Autores principales:	Jian Chen, Huamei Tang, Zehua Wu, Chongzhi Zhou, Tao Jiang, Yingming Xue, Guoyu Huang, Dongwang Yan, Zhihai Peng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/151071bc96d94b90ae834101d6651cbc
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spelling	oai:doaj.org-article:151071bc96d94b90ae834101d6651cbc2021-11-18T07:41:32ZOverexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.1932-620310.1371/journal.pone.0066524https://doaj.org/article/151071bc96d94b90ae834101d6651cbc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23799110/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Retinoblastoma binding protein 6 (RBBP6) plays an important role in chaperone-mediated ubiquitination and interacts with TP53 in carcinogenesis. However, the clinicopathologic significance of RBBP6 expression in colon cancer is unknown; in particular, the prognostic value of RBBP6 combined with TP53 expression has not been explored. Therefore, quantitative real-time PCR and western blot analyses were performed to detect RBBP6 expression in colon cancer tissues. RBBP6 and TP53 expression were assessed by immunohistochemistry in a tissue microarray format, in which the primary colon cancer tissue was paired with noncancerous tissue. Tissue specimens were obtained from 203 patients. We found that RBBP6 was overexpressed in colon tumorous tissues and was significantly associated with clinical stage, depth of tumor invasion, lymph node metastasis (LNM), distant metastasis, and histologic grade. Further studies revealed that a corresponding correlation between RBBP6 overexpression and mutant TP53 was evident in colon cancer (r = 0.450; P<0.001). RBBP6 expression was an independent prognostic factor for overall survival (OS) and disease free survival (DFS). Interestingly, patients with tumors that had both RBBP6 overexpression and mutant TP53 protein accumulation relapsed and died within a significantly short period after surgery (P<0.001). Multivariate analysis showed that patients with LNM and patients with both RBBP6- and TP53-positive tumors had extremely poor OS (HR 6.75; 95% CI 2.63-17.35; P<0.001) and DFS (HR 8.08; 95% CI 2.80-23.30; P<0.001). These clinical findings indicate that the assessment of both RBBP6 and mutant TP53 expression will be helpful in predicting colon cancer prognosis.Jian ChenHuamei TangZehua WuChongzhi ZhouTao JiangYingming XueGuoyu HuangDongwang YanZhihai PengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66524 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Chen Huamei Tang Zehua Wu Chongzhi Zhou Tao Jiang Yingming Xue Guoyu Huang Dongwang Yan Zhihai Peng Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
description	Retinoblastoma binding protein 6 (RBBP6) plays an important role in chaperone-mediated ubiquitination and interacts with TP53 in carcinogenesis. However, the clinicopathologic significance of RBBP6 expression in colon cancer is unknown; in particular, the prognostic value of RBBP6 combined with TP53 expression has not been explored. Therefore, quantitative real-time PCR and western blot analyses were performed to detect RBBP6 expression in colon cancer tissues. RBBP6 and TP53 expression were assessed by immunohistochemistry in a tissue microarray format, in which the primary colon cancer tissue was paired with noncancerous tissue. Tissue specimens were obtained from 203 patients. We found that RBBP6 was overexpressed in colon tumorous tissues and was significantly associated with clinical stage, depth of tumor invasion, lymph node metastasis (LNM), distant metastasis, and histologic grade. Further studies revealed that a corresponding correlation between RBBP6 overexpression and mutant TP53 was evident in colon cancer (r = 0.450; P<0.001). RBBP6 expression was an independent prognostic factor for overall survival (OS) and disease free survival (DFS). Interestingly, patients with tumors that had both RBBP6 overexpression and mutant TP53 protein accumulation relapsed and died within a significantly short period after surgery (P<0.001). Multivariate analysis showed that patients with LNM and patients with both RBBP6- and TP53-positive tumors had extremely poor OS (HR 6.75; 95% CI 2.63-17.35; P<0.001) and DFS (HR 8.08; 95% CI 2.80-23.30; P<0.001). These clinical findings indicate that the assessment of both RBBP6 and mutant TP53 expression will be helpful in predicting colon cancer prognosis.
format	article
author	Jian Chen Huamei Tang Zehua Wu Chongzhi Zhou Tao Jiang Yingming Xue Guoyu Huang Dongwang Yan Zhihai Peng
author_facet	Jian Chen Huamei Tang Zehua Wu Chongzhi Zhou Tao Jiang Yingming Xue Guoyu Huang Dongwang Yan Zhihai Peng
author_sort	Jian Chen
title	Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
title_short	Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
title_full	Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
title_fullStr	Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
title_full_unstemmed	Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.
title_sort	overexpression of rbbp6, alone or combined with mutant tp53, is predictive of poor prognosis in colon cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/151071bc96d94b90ae834101d6651cbc
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Overexpression of RBBP6, alone or combined with mutant TP53, is predictive of poor prognosis in colon cancer.

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