Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells

Abstract Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the...

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Autores principales:	Hubert M. Taïeb, Daniela S. Garske, Jörg Contzen, Manfred Gossen, Luca Bertinetti, Tom Robinson, Amaia Cipitria
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1514b2a9b79e4d038cb3e1de5d69c3ac
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spelling	oai:doaj.org-article:1514b2a9b79e4d038cb3e1de5d69c3ac2021-12-02T18:18:51ZOsmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells10.1038/s41598-021-92054-w2045-2322https://doaj.org/article/1514b2a9b79e4d038cb3e1de5d69c3ac2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92054-whttps://doaj.org/toc/2045-2322Abstract Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of osmotic pressure on single cell cycle dynamics remains poorly understood. Here, we investigate the effect of osmotic pressure on single cell cycle dynamics, nuclear growth, proliferation, migration and protein expression, by quantitative time-lapse imaging of single cells genetically modified with fluorescent ubiquitination-based cell cycle indicator 2 (FUCCI2). Single cell data reveals that under hyperosmotic stress, distinct cell subpopulations emerge with impaired nuclear growth, delayed or growth arrested cell cycle and reduced migration. This state is reversible for mild hyperosmotic stress, where cells return to regular cell cycle dynamics, proliferation and migration. Thus, osmotic pressure can modulate the reversible growth arrest and reactivation of human metastatic cells.Hubert M. TaïebDaniela S. GarskeJörg ContzenManfred GossenLuca BertinettiTom RobinsonAmaia CipitriaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hubert M. Taïeb Daniela S. Garske Jörg Contzen Manfred Gossen Luca Bertinetti Tom Robinson Amaia Cipitria Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
description	Abstract Biophysical cues such as osmotic pressure modulate proliferation and growth arrest of bacteria, yeast cells and seeds. In tissues, osmotic regulation takes place through blood and lymphatic capillaries and, at a single cell level, water and osmoregulation play a critical role. However, the effect of osmotic pressure on single cell cycle dynamics remains poorly understood. Here, we investigate the effect of osmotic pressure on single cell cycle dynamics, nuclear growth, proliferation, migration and protein expression, by quantitative time-lapse imaging of single cells genetically modified with fluorescent ubiquitination-based cell cycle indicator 2 (FUCCI2). Single cell data reveals that under hyperosmotic stress, distinct cell subpopulations emerge with impaired nuclear growth, delayed or growth arrested cell cycle and reduced migration. This state is reversible for mild hyperosmotic stress, where cells return to regular cell cycle dynamics, proliferation and migration. Thus, osmotic pressure can modulate the reversible growth arrest and reactivation of human metastatic cells.
format	article
author	Hubert M. Taïeb Daniela S. Garske Jörg Contzen Manfred Gossen Luca Bertinetti Tom Robinson Amaia Cipitria
author_facet	Hubert M. Taïeb Daniela S. Garske Jörg Contzen Manfred Gossen Luca Bertinetti Tom Robinson Amaia Cipitria
author_sort	Hubert M. Taïeb
title	Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_short	Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_full	Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_fullStr	Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_full_unstemmed	Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
title_sort	osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1514b2a9b79e4d038cb3e1de5d69c3ac
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_version_	1718378138918977536

Osmotic pressure modulates single cell cycle dynamics inducing reversible growth arrest and reactivation of human metastatic cells

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