FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins

Summary: We have developed a fabrication methodology for label-free optical trapping of individual nanobeads and proteins in inverted-bowtie-shaped plasmonic gold nanopores. Arrays of these nanoapertures can be reliably produced using focused ion beam (FIB) milling with gap sizes of 10–20 nm, single...

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Autores principales:	Wayne Yang, Madeleine van Dijk, Christian Primavera, Cees Dekker
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Physical chemistry Biophysical chemistry Protein Materials science Science Q
Acceso en línea:	https://doaj.org/article/15159dba01ce46d8b6166051af8734ae
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spelling	oai:doaj.org-article:15159dba01ce46d8b6166051af8734ae2021-11-20T05:08:42ZFIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins2589-004210.1016/j.isci.2021.103237https://doaj.org/article/15159dba01ce46d8b6166051af8734ae2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012050https://doaj.org/toc/2589-0042Summary: We have developed a fabrication methodology for label-free optical trapping of individual nanobeads and proteins in inverted-bowtie-shaped plasmonic gold nanopores. Arrays of these nanoapertures can be reliably produced using focused ion beam (FIB) milling with gap sizes of 10–20 nm, single-nanometer variation, and with a remarkable stability that allows for repeated use. We employ an optical readout where the presence of the protein entering the trap is marked by an increase in the transmission of light through the nanoaperture from the shift of the plasmonic resonance. In addition, the optical trapping force of the plasmonic nanopores allows 20-nm polystyrene beads and proteins, such as beta-amylase and Heat Shock Protein (HSP90), to be trapped for very long times (approximately minutes). On demand, we can release the trapped molecule for another protein to be interrogated. Our work opens up new routes to acquire information on the conformation and dynamics of individual proteins.Wayne YangMadeleine van DijkChristian PrimaveraCees DekkerElsevierarticlePhysical chemistryBiophysical chemistryProteinMaterials scienceScienceQENiScience, Vol 24, Iss 11, Pp 103237- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physical chemistry Biophysical chemistry Protein Materials science Science Q
spellingShingle	Physical chemistry Biophysical chemistry Protein Materials science Science Q Wayne Yang Madeleine van Dijk Christian Primavera Cees Dekker FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
description	Summary: We have developed a fabrication methodology for label-free optical trapping of individual nanobeads and proteins in inverted-bowtie-shaped plasmonic gold nanopores. Arrays of these nanoapertures can be reliably produced using focused ion beam (FIB) milling with gap sizes of 10–20 nm, single-nanometer variation, and with a remarkable stability that allows for repeated use. We employ an optical readout where the presence of the protein entering the trap is marked by an increase in the transmission of light through the nanoaperture from the shift of the plasmonic resonance. In addition, the optical trapping force of the plasmonic nanopores allows 20-nm polystyrene beads and proteins, such as beta-amylase and Heat Shock Protein (HSP90), to be trapped for very long times (approximately minutes). On demand, we can release the trapped molecule for another protein to be interrogated. Our work opens up new routes to acquire information on the conformation and dynamics of individual proteins.
format	article
author	Wayne Yang Madeleine van Dijk Christian Primavera Cees Dekker
author_facet	Wayne Yang Madeleine van Dijk Christian Primavera Cees Dekker
author_sort	Wayne Yang
title	FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
title_short	FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
title_full	FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
title_fullStr	FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
title_full_unstemmed	FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins
title_sort	fib-milled plasmonic nanoapertures allow for long trapping times of individual proteins
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/15159dba01ce46d8b6166051af8734ae
work_keys_str_mv	AT wayneyang fibmilledplasmonicnanoaperturesallowforlongtrappingtimesofindividualproteins AT madeleinevandijk fibmilledplasmonicnanoaperturesallowforlongtrappingtimesofindividualproteins AT christianprimavera fibmilledplasmonicnanoaperturesallowforlongtrappingtimesofindividualproteins AT ceesdekker fibmilledplasmonicnanoaperturesallowforlongtrappingtimesofindividualproteins
_version_	1718419526124568576

FIB-milled plasmonic nanoapertures allow for long trapping times of individual proteins

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