Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]

Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Manjeet Singh, Ram Avtar, Nita Lakra, Ekta Hooda, Vivek K. Singh, Mahavir Bishnoi, Nisha Kumari, Rakesh Punia, Neeraj Kumar, Raju Ram Choudhary
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Sclerotinia stem rot Indian mustard pathogen resistance generation mean analysis PPIase protein Genetics QH426-470
Acceso en línea:	https://doaj.org/article/151a34c8619142cfa2e62b262604d27a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:151a34c8619142cfa2e62b262604d27a
record_format	dspace
spelling	oai:doaj.org-article:151a34c8619142cfa2e62b262604d27a2021-11-25T17:41:57ZGenetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]10.3390/genes121117842073-4425https://doaj.org/article/151a34c8619142cfa2e62b262604d27a2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1784https://doaj.org/toc/2073-4425Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P<sub>1</sub>, P<sub>2</sub>, F<sub>1</sub>, F<sub>2</sub>, BC<sub>1</sub>P<sub>1</sub>, and BC<sub>1</sub>P<sub>2</sub>) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding programs to develop resistant cultivars against this devastating disease.Manjeet SinghRam AvtarNita LakraEkta HoodaVivek K. SinghMahavir BishnoiNisha KumariRakesh PuniaNeeraj KumarRaju Ram ChoudharyMDPI AGarticleSclerotinia stem rotIndian mustardpathogen resistancegeneration mean analysisPPIaseproteinGeneticsQH426-470ENGenes, Vol 12, Iss 1784, p 1784 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sclerotinia stem rot Indian mustard pathogen resistance generation mean analysis PPIase protein Genetics QH426-470
spellingShingle	Sclerotinia stem rot Indian mustard pathogen resistance generation mean analysis PPIase protein Genetics QH426-470 Manjeet Singh Ram Avtar Nita Lakra Ekta Hooda Vivek K. Singh Mahavir Bishnoi Nisha Kumari Rakesh Punia Neeraj Kumar Raju Ram Choudhary Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
description	Sclerotinia stem rot is one of the utmost important disease of mustard, causing considerable losses in seed yield and oil quality. The study of the genetic and proteomic basis of resistance to this disease is imperative for its effective utilization in developing resistant cultivars. Therefore, the genetic pattern of Sclerotinia stem rot resistance in Indian mustard was studied using six generations (P<sub>1</sub>, P<sub>2</sub>, F<sub>1</sub>, F<sub>2</sub>, BC<sub>1</sub>P<sub>1</sub>, and BC<sub>1</sub>P<sub>2</sub>) developed from the crossing of one resistant (RH 1222-28) and two susceptible (EC 766300 and EC 766123) genotypes. Genetic analysis revealed that resistance was governed by duplicate epistasis. Comparative proteome analysis of resistant and susceptible genotypes indicated that peptidyl-prolyl cis-trans isomerase (A0A078IDN6 PPIase) showed high expression in resistant genotype at the early infection stage while its expression was delayed in susceptible genotypes. This study provides important insight to mustard breeders for designing effective breeding programs to develop resistant cultivars against this devastating disease.
format	article
author	Manjeet Singh Ram Avtar Nita Lakra Ekta Hooda Vivek K. Singh Mahavir Bishnoi Nisha Kumari Rakesh Punia Neeraj Kumar Raju Ram Choudhary
author_facet	Manjeet Singh Ram Avtar Nita Lakra Ekta Hooda Vivek K. Singh Mahavir Bishnoi Nisha Kumari Rakesh Punia Neeraj Kumar Raju Ram Choudhary
author_sort	Manjeet Singh
title	Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
title_short	Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
title_full	Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
title_fullStr	Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
title_full_unstemmed	Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]
title_sort	genetic and proteomic basis of sclerotinia stem rot resistance in indian mustard [<i>brassica juncea</i> (l.) czern & coss.]
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/151a34c8619142cfa2e62b262604d27a
work_keys_str_mv	AT manjeetsingh geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT ramavtar geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT nitalakra geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT ektahooda geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT vivekksingh geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT mahavirbishnoi geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT nishakumari geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT rakeshpunia geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT neerajkumar geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss AT rajuramchoudhary geneticandproteomicbasisofsclerotiniastemrotresistanceinindianmustardibrassicajunceailczerncoss
_version_	1718412090035666944

Genetic and Proteomic Basis of Sclerotinia Stem Rot Resistance in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]

Ejemplares similares