Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo

Abstract Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without the need of delivering or inserting any exogenous agent into the tissue. Very few studies have explored its use in the brain, as most investigations...

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Autores principales:	Attila Kaszas, Gergely Szalay, Andrea Slézia, Alexandra Bojdán, Ivo Vanzetta, Balázs Hangya, Balázs Rózsa, Rodney O’Connor, David Moreau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/151ba016468243b6b2b5ae94c6eb61db
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spelling	oai:doaj.org-article:151ba016468243b6b2b5ae94c6eb61db2021-12-02T14:42:52ZTwo-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo10.1038/s41598-021-89163-x2045-2322https://doaj.org/article/151ba016468243b6b2b5ae94c6eb61db2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89163-xhttps://doaj.org/toc/2045-2322Abstract Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without the need of delivering or inserting any exogenous agent into the tissue. Very few studies have explored its use in the brain, as most investigations have focused on sensory or motor nerve stimulation. Using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f, here we show that the application of infrared neural stimulation induces intracellular calcium signals in Layer 2/3 neurons in mouse cortex in vivo. The number of neurons exhibiting infrared-induced calcium response as well as the amplitude of those signals are shown to be both increasing with the energy density applied. By studying as well the spatial extent of the stimulation, we show that reproducibility of the stimulation is achieved mainly in the central part of the infrared beam path. Stimulating in vivo at such a degree of precision and without any exogenous chromophores enables multiple applications, from mapping the brain’s connectome to applications in systems neuroscience and the development of new therapeutic tools for investigating the pathological brain.Attila KaszasGergely SzalayAndrea SléziaAlexandra BojdánIvo VanzettaBalázs HangyaBalázs RózsaRodney O’ConnorDavid MoreauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Attila Kaszas Gergely Szalay Andrea Slézia Alexandra Bojdán Ivo Vanzetta Balázs Hangya Balázs Rózsa Rodney O’Connor David Moreau Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
description	Abstract Infrared neural stimulation is a promising tool for stimulating the brain because it can be used to excite with high spatial precision without the need of delivering or inserting any exogenous agent into the tissue. Very few studies have explored its use in the brain, as most investigations have focused on sensory or motor nerve stimulation. Using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f, here we show that the application of infrared neural stimulation induces intracellular calcium signals in Layer 2/3 neurons in mouse cortex in vivo. The number of neurons exhibiting infrared-induced calcium response as well as the amplitude of those signals are shown to be both increasing with the energy density applied. By studying as well the spatial extent of the stimulation, we show that reproducibility of the stimulation is achieved mainly in the central part of the infrared beam path. Stimulating in vivo at such a degree of precision and without any exogenous chromophores enables multiple applications, from mapping the brain’s connectome to applications in systems neuroscience and the development of new therapeutic tools for investigating the pathological brain.
format	article
author	Attila Kaszas Gergely Szalay Andrea Slézia Alexandra Bojdán Ivo Vanzetta Balázs Hangya Balázs Rózsa Rodney O’Connor David Moreau
author_facet	Attila Kaszas Gergely Szalay Andrea Slézia Alexandra Bojdán Ivo Vanzetta Balázs Hangya Balázs Rózsa Rodney O’Connor David Moreau
author_sort	Attila Kaszas
title	Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
title_short	Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
title_full	Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
title_fullStr	Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
title_full_unstemmed	Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
title_sort	two-photon gcamp6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/151ba016468243b6b2b5ae94c6eb61db
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Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo

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