Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice

AIM: To investigate the effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia(FDM)mice.METHODS: Totally 72 C57BL/6J mice aged 3-week-old were randomly divided into control group 1, model group 1, intervention group 1, control group 2, model group 2 and interve...

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Autores principales:	Ya Mo, Guo-Ting Ren, Xi-Yuan Deng, Jie Ma, Shi-Yun Tang, Lyu-Lyu Zhou, Xi-Li Xiao, Qun Huang
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	form deprivation myopia c57bl/6j mice retinal thickness apoptosis modified zhujing pill Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/1524b217d08648b297397a243e79d8dd
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id	oai:doaj.org-article:1524b217d08648b297397a243e79d8dd
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	form deprivation myopia c57bl/6j mice retinal thickness apoptosis modified zhujing pill Ophthalmology RE1-994
spellingShingle	form deprivation myopia c57bl/6j mice retinal thickness apoptosis modified zhujing pill Ophthalmology RE1-994 Ya Mo Guo-Ting Ren Xi-Yuan Deng Jie Ma Shi-Yun Tang Lyu-Lyu Zhou Xi-Li Xiao Qun Huang Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
description	AIM: To investigate the effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia(FDM)mice.METHODS: Totally 72 C57BL/6J mice aged 3-week-old were randomly divided into control group 1, model group 1, intervention group 1, control group 2, model group 2 and intervention group 2, with 12 mice in each group. The first three groups were tested for 3wk and the last three groups were tested for 6wk, except for the groups of control 1 and control 2, all the mice used translucent goggles to cover their right eyes for form deprivation. The mice of intervention 1 and intervention 2 were respectively given intragastric administration modified Zhujing pill suspension 0.546g/(kg·d)(0.1mL/d)for 3wk at the beginning and after 3wk of the experiment. Same amount of saline was given to mice in other groups at the same time of modeling. The eye axis was measured before and after the experiment. At the end of the experiment, the eye of mice was taken for HE staining to observe the thickness changes of each layer of retina. Immunohistochemistry(IHC)and western blotting(WB)were used to detect Bcl-2 and Caspase3 expression of protein.RESULTS: At the end of the experiment, the axis of model group 1 was significantly higher than that of control group 1(P<0.01), the axis of intervention group 1 was significantly lower than that of model group 1(P<0.01), and the axis of model group 2 was significantly higher than that of control group 2(P<0.01), the eye axis of intervention group 2 was significantly lower than that of model group 2(P<0.01); HE staining showed that the thickness of NFL and ONL of model group 1 was significantly thinner than that of control group 1(P<0.01). The thickness of INL of model group 1 was significantly thinner than that of control group 1(P<0.05), and the thickness of NFL, INL and ONL of intervention group 1 was significantly higher than that of model group 1(P<0.05); The thickness of NFL, INL and ONL model group 2 was significantly thinner than that of control group 1(P<0.01); IHC testing showed that the average optical density of Bcl-2 protein in model group 1 was significantly lower than that of control group 1(P<0.05), which in intervention group 1 was significantly higher than that of model group 1(P<0.01), and which in the average optical density of Bcl-2 protein of model group 2 was significantly lower than that of control group 2(P<0.01), which in intervention group 2 was significantly higher than that of model group 2(P<0.01); Caspase 3 protein average optical density of model group 1 was significantly higher than that of control group 1(P<0.01), which in intervention group 1 was significantly lower than that of model group 1(P<0.01), which in model group 2 was significantly higher than that of control group 2(P<0.05), which in intervention group 2 was significantly lower than that of model group 2(P<0.01); WB test proved that the relative expression level of Bcl-2 protein in model group 1 was significantly lower than that of control group 1(P<0.01), which in intervention group 1 was significantly higher than that of model group 1(P<0.01), and which in model group 2 was significantly lower than that of control group 2(P<0.01), which in intervention group 2 was significantly higher than that of model group 2(P<0.01); The relative expression level of Caspase3 protein in model group 1 was significantly higher than that of control group 1(P<0.01), which in intervention group 1 was significantly lower than that of model group 1(P<0.01), the intervention group 2 was significantly lower than that of model group 2. CONCLUSION: The results show that the modified Zhujing pill can interfere with the pathological changes of retinal thickness thinning in the process of myopia and formed myopia mice by regulating the expression of apoptosis-related proteins Bcl-2 and Caspase3, and alleviating the apoptosis of retinal cells in myopia formation and myopia mice.
format	article
author	Ya Mo Guo-Ting Ren Xi-Yuan Deng Jie Ma Shi-Yun Tang Lyu-Lyu Zhou Xi-Li Xiao Qun Huang
author_facet	Ya Mo Guo-Ting Ren Xi-Yuan Deng Jie Ma Shi-Yun Tang Lyu-Lyu Zhou Xi-Li Xiao Qun Huang
author_sort	Ya Mo
title	Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
title_short	Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
title_full	Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
title_fullStr	Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
title_full_unstemmed	Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
title_sort	effect of modified zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/1524b217d08648b297397a243e79d8dd
work_keys_str_mv	AT yamo effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT guotingren effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT xiyuandeng effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT jiema effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT shiyuntang effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT lyulyuzhou effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT xilixiao effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice AT qunhuang effectofmodifiedzhujingpillonretinalthicknessandapoptosisinformdeprivationmyopiamice
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spelling	oai:doaj.org-article:1524b217d08648b297397a243e79d8dd2021-11-22T14:22:32ZEffect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice1672-512310.3980/j.issn.1672-5123.2021.12.06https://doaj.org/article/1524b217d08648b297397a243e79d8dd2021-12-01T00:00:00Zhttp://ies.ijo.cn/cn_publish/2021/12/202112006.pdfhttps://doaj.org/toc/1672-5123AIM: To investigate the effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia(FDM)mice.METHODS: Totally 72 C57BL/6J mice aged 3-week-old were randomly divided into control group 1, model group 1, intervention group 1, control group 2, model group 2 and intervention group 2, with 12 mice in each group. The first three groups were tested for 3wk and the last three groups were tested for 6wk, except for the groups of control 1 and control 2, all the mice used translucent goggles to cover their right eyes for form deprivation. The mice of intervention 1 and intervention 2 were respectively given intragastric administration modified Zhujing pill suspension 0.546g/(kg·d)(0.1mL/d)for 3wk at the beginning and after 3wk of the experiment. Same amount of saline was given to mice in other groups at the same time of modeling. The eye axis was measured before and after the experiment. At the end of the experiment, the eye of mice was taken for HE staining to observe the thickness changes of each layer of retina. Immunohistochemistry(IHC)and western blotting(WB)were used to detect Bcl-2 and Caspase3 expression of protein.RESULTS: At the end of the experiment, the axis of model group 1 was significantly higher than that of control group 1(P<0.01), the axis of intervention group 1 was significantly lower than that of model group 1(P<0.01), and the axis of model group 2 was significantly higher than that of control group 2(P<0.01), the eye axis of intervention group 2 was significantly lower than that of model group 2(P<0.01); HE staining showed that the thickness of NFL and ONL of model group 1 was significantly thinner than that of control group 1(P<0.01). The thickness of INL of model group 1 was significantly thinner than that of control group 1(P<0.05), and the thickness of NFL, INL and ONL of intervention group 1 was significantly higher than that of model group 1(P<0.05); The thickness of NFL, INL and ONL model group 2 was significantly thinner than that of control group 1(P<0.01); IHC testing showed that the average optical density of Bcl-2 protein in model group 1 was significantly lower than that of control group 1(P<0.05), which in intervention group 1 was significantly higher than that of model group 1(P<0.01), and which in the average optical density of Bcl-2 protein of model group 2 was significantly lower than that of control group 2(P<0.01), which in intervention group 2 was significantly higher than that of model group 2(P<0.01); Caspase 3 protein average optical density of model group 1 was significantly higher than that of control group 1(P<0.01), which in intervention group 1 was significantly lower than that of model group 1(P<0.01), which in model group 2 was significantly higher than that of control group 2(P<0.05), which in intervention group 2 was significantly lower than that of model group 2(P<0.01); WB test proved that the relative expression level of Bcl-2 protein in model group 1 was significantly lower than that of control group 1(P<0.01), which in intervention group 1 was significantly higher than that of model group 1(P<0.01), and which in model group 2 was significantly lower than that of control group 2(P<0.01), which in intervention group 2 was significantly higher than that of model group 2(P<0.01); The relative expression level of Caspase3 protein in model group 1 was significantly higher than that of control group 1(P<0.01), which in intervention group 1 was significantly lower than that of model group 1(P<0.01), the intervention group 2 was significantly lower than that of model group 2. CONCLUSION: The results show that the modified Zhujing pill can interfere with the pathological changes of retinal thickness thinning in the process of myopia and formed myopia mice by regulating the expression of apoptosis-related proteins Bcl-2 and Caspase3, and alleviating the apoptosis of retinal cells in myopia formation and myopia mice.Ya MoGuo-Ting RenXi-Yuan DengJie MaShi-Yun TangLyu-Lyu ZhouXi-Li XiaoQun HuangPress of International Journal of Ophthalmology (IJO PRESS)articleform deprivation myopiac57bl/6j miceretinal thicknessapoptosismodified zhujing pillOphthalmologyRE1-994ENGuoji Yanke Zazhi, Vol 21, Iss 12, Pp 2053-2059 (2021)

Effect of modified Zhujing pill on retinal thickness and apoptosis in form deprivation myopia mice

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