Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle

Abstract The bacteriophage infection cycle has been extensively studied, yet little is known about the nanostructure and mechanical changes that lead to bacterial lysis. Here, atomic force microscopy was used to study in real time and in situ the impact of the canonical phage T4 on the nanotopograph...

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Autores principales:	Shiju Abraham, Yair Kaufman, François Perreault, Ry Young, Edo Bar-Zeev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/152b8e4c107045aaa1f3beaefc6f9e40
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spelling	oai:doaj.org-article:152b8e4c107045aaa1f3beaefc6f9e402021-12-02T16:31:03ZBursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle10.1038/s41522-021-00195-72055-5008https://doaj.org/article/152b8e4c107045aaa1f3beaefc6f9e402021-03-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00195-7https://doaj.org/toc/2055-5008Abstract The bacteriophage infection cycle has been extensively studied, yet little is known about the nanostructure and mechanical changes that lead to bacterial lysis. Here, atomic force microscopy was used to study in real time and in situ the impact of the canonical phage T4 on the nanotopography and biomechanics of irreversibly attached, biofilm-forming E. coli cells. The results show that in contrast to the lytic cycle in planktonic cells, which ends explosively, anchored cells that are in the process of forming a biofilm undergo a more gradual lysis, developing distinct nanoscale lesions (~300 nm in diameter) within the cell envelope. Furthermore, it is shown that the envelope rigidity and cell elasticity decrease (>50% and >40%, respectively) following T4 infection, a process likely linked to changes in the nanostructure of infected cells. These insights show that the well-established lytic pathway of planktonic cells may be significantly different from that of biofilm-forming cells. Elucidating the lysis paradigm of these cells may advance biofilm removal and phage therapeutics.Shiju AbrahamYair KaufmanFrançois PerreaultRy YoungEdo Bar-ZeevNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Shiju Abraham Yair Kaufman François Perreault Ry Young Edo Bar-Zeev Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
description	Abstract The bacteriophage infection cycle has been extensively studied, yet little is known about the nanostructure and mechanical changes that lead to bacterial lysis. Here, atomic force microscopy was used to study in real time and in situ the impact of the canonical phage T4 on the nanotopography and biomechanics of irreversibly attached, biofilm-forming E. coli cells. The results show that in contrast to the lytic cycle in planktonic cells, which ends explosively, anchored cells that are in the process of forming a biofilm undergo a more gradual lysis, developing distinct nanoscale lesions (~300 nm in diameter) within the cell envelope. Furthermore, it is shown that the envelope rigidity and cell elasticity decrease (>50% and >40%, respectively) following T4 infection, a process likely linked to changes in the nanostructure of infected cells. These insights show that the well-established lytic pathway of planktonic cells may be significantly different from that of biofilm-forming cells. Elucidating the lysis paradigm of these cells may advance biofilm removal and phage therapeutics.
format	article
author	Shiju Abraham Yair Kaufman François Perreault Ry Young Edo Bar-Zeev
author_facet	Shiju Abraham Yair Kaufman François Perreault Ry Young Edo Bar-Zeev
author_sort	Shiju Abraham
title	Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
title_short	Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
title_full	Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
title_fullStr	Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
title_full_unstemmed	Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle
title_sort	bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming escherichia coli to the t4 lytic cycle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/152b8e4c107045aaa1f3beaefc6f9e40
work_keys_str_mv	AT shijuabraham burstingoutlinkingchangesinnanotopographyandbiomechanicalpropertiesofbiofilmformingescherichiacolitothet4lyticcycle AT yairkaufman burstingoutlinkingchangesinnanotopographyandbiomechanicalpropertiesofbiofilmformingescherichiacolitothet4lyticcycle AT francoisperreault burstingoutlinkingchangesinnanotopographyandbiomechanicalpropertiesofbiofilmformingescherichiacolitothet4lyticcycle AT ryyoung burstingoutlinkingchangesinnanotopographyandbiomechanicalpropertiesofbiofilmformingescherichiacolitothet4lyticcycle AT edobarzeev burstingoutlinkingchangesinnanotopographyandbiomechanicalpropertiesofbiofilmformingescherichiacolitothet4lyticcycle
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Bursting out: linking changes in nanotopography and biomechanical properties of biofilm-forming Escherichia coli to the T4 lytic cycle

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