iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat

Abstract By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F10 recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive d...

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Autores principales:	Ning Zhang, Lingran Zhang, Lei Zhao, Yan Ren, Dangqun Cui, Jianhui Chen, Yongyan Wang, Pengbo Yu, Feng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/152e363746ba477ba669c1a5f7c84b95
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spelling	oai:doaj.org-article:152e363746ba477ba669c1a5f7c84b952021-12-02T11:41:22ZiTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat10.1038/s41598-017-08069-92045-2322https://doaj.org/article/152e363746ba477ba669c1a5f7c84b952017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08069-9https://doaj.org/toc/2045-2322Abstract By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F10 recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive descendant lines with 10 cold-tolerant descendant lines identified 140 proteins that showed decreased protein abundance, such as the components of the photosynthesis apparatus and cell-wall metabolism. The identified proteins were classified into the following main groups: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, sulfur metabolism, nitrogen metabolism, RNA metabolism, energy production, cell-wall metabolism, membrane and transportation, and signal transduction. Results of quantitative real-time PCR of 20 differentially accumulated proteins indicated that the transcriptional expression patterns of 10 genes were consistent with their protein expression models. Virus-induced gene silencing of Hsp90, BBI, and REP14 genes indicated that virus-silenced plants subjected to cold stress had more severe drooping and wilting, an increased rate of relative electrolyte leakage, and reduced relative water content compared to viral control plants. Furthermore, ultrastructural changes of virus-silenced plants were destroyed more severely than those of viral control plants. These results indicate that Hsp90, BBI, and REP14 potentially play vital roles in conferring cold tolerance in bread wheat.Ning ZhangLingran ZhangLei ZhaoYan RenDangqun CuiJianhui ChenYongyan WangPengbo YuFeng ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ning Zhang Lingran Zhang Lei Zhao Yan Ren Dangqun Cui Jianhui Chen Yongyan Wang Pengbo Yu Feng Chen iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
description	Abstract By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F10 recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive descendant lines with 10 cold-tolerant descendant lines identified 140 proteins that showed decreased protein abundance, such as the components of the photosynthesis apparatus and cell-wall metabolism. The identified proteins were classified into the following main groups: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, sulfur metabolism, nitrogen metabolism, RNA metabolism, energy production, cell-wall metabolism, membrane and transportation, and signal transduction. Results of quantitative real-time PCR of 20 differentially accumulated proteins indicated that the transcriptional expression patterns of 10 genes were consistent with their protein expression models. Virus-induced gene silencing of Hsp90, BBI, and REP14 genes indicated that virus-silenced plants subjected to cold stress had more severe drooping and wilting, an increased rate of relative electrolyte leakage, and reduced relative water content compared to viral control plants. Furthermore, ultrastructural changes of virus-silenced plants were destroyed more severely than those of viral control plants. These results indicate that Hsp90, BBI, and REP14 potentially play vital roles in conferring cold tolerance in bread wheat.
format	article
author	Ning Zhang Lingran Zhang Lei Zhao Yan Ren Dangqun Cui Jianhui Chen Yongyan Wang Pengbo Yu Feng Chen
author_facet	Ning Zhang Lingran Zhang Lei Zhao Yan Ren Dangqun Cui Jianhui Chen Yongyan Wang Pengbo Yu Feng Chen
author_sort	Ning Zhang
title	iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
title_short	iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
title_full	iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
title_fullStr	iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
title_full_unstemmed	iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
title_sort	itraq and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/152e363746ba477ba669c1a5f7c84b95
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iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat

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