Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation

Abstract Perfluoroalkyl substances (PFAS), including two most commonly studied compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are widely distributed environmental pollutants, used extensively earlier. Due to their toxicological effects the use of PFAS is now regulated....

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Autores principales:	A. Koskela, J. Koponen, P. Lehenkari, M. Viluksela, M. Korkalainen, J. Tuukkanen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/152e8ddd4f1b498ab49083bf656f58ae
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spelling	oai:doaj.org-article:152e8ddd4f1b498ab49083bf656f58ae2021-12-02T16:05:58ZPerfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation10.1038/s41598-017-07359-62045-2322https://doaj.org/article/152e8ddd4f1b498ab49083bf656f58ae2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07359-6https://doaj.org/toc/2045-2322Abstract Perfluoroalkyl substances (PFAS), including two most commonly studied compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are widely distributed environmental pollutants, used extensively earlier. Due to their toxicological effects the use of PFAS is now regulated. Based on earlier studies on PFOA’s distribution in bone and bone marrow in mice, we investigated PFAS levels and their possible link to bone microarchitecture of human femoral bone samples (n = 18). Soft tissue and bone biopsies were also taken from a 49-year old female cadaver for PFAS analyses. We also studied how PFOA exposure affects differentiation of human osteoblasts and osteoclasts. PFAS were detectable from all dry bone and bone marrow samples, PFOS and PFOA being the most prominent. In cadaver biopsies, lungs and liver contained the highest concentrations of PFAS, whereas PFAS were absent in bone marrow. Perfluorononanoic acid (PFNA) was present in the bones, PFOA and PFOS were absent. In vitro results showed no disturbance in osteogenic differentiation after PFOA exposure, but in osteoclasts, lower concentrations led to increased resorption, which eventually dropped to zero after increase in PFOA concentration. In conclusion, PFAS are present in bone and have the potential to affect human bone cells partly at environmentally relevant concentrations.A. KoskelaJ. KoponenP. LehenkariM. VilukselaM. KorkalainenJ. TuukkanenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Koskela J. Koponen P. Lehenkari M. Viluksela M. Korkalainen J. Tuukkanen Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
description	Abstract Perfluoroalkyl substances (PFAS), including two most commonly studied compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are widely distributed environmental pollutants, used extensively earlier. Due to their toxicological effects the use of PFAS is now regulated. Based on earlier studies on PFOA’s distribution in bone and bone marrow in mice, we investigated PFAS levels and their possible link to bone microarchitecture of human femoral bone samples (n = 18). Soft tissue and bone biopsies were also taken from a 49-year old female cadaver for PFAS analyses. We also studied how PFOA exposure affects differentiation of human osteoblasts and osteoclasts. PFAS were detectable from all dry bone and bone marrow samples, PFOS and PFOA being the most prominent. In cadaver biopsies, lungs and liver contained the highest concentrations of PFAS, whereas PFAS were absent in bone marrow. Perfluorononanoic acid (PFNA) was present in the bones, PFOA and PFOS were absent. In vitro results showed no disturbance in osteogenic differentiation after PFOA exposure, but in osteoclasts, lower concentrations led to increased resorption, which eventually dropped to zero after increase in PFOA concentration. In conclusion, PFAS are present in bone and have the potential to affect human bone cells partly at environmentally relevant concentrations.
format	article
author	A. Koskela J. Koponen P. Lehenkari M. Viluksela M. Korkalainen J. Tuukkanen
author_facet	A. Koskela J. Koponen P. Lehenkari M. Viluksela M. Korkalainen J. Tuukkanen
author_sort	A. Koskela
title	Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
title_short	Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
title_full	Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
title_fullStr	Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
title_full_unstemmed	Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
title_sort	perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/152e8ddd4f1b498ab49083bf656f58ae
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Perfluoroalkyl substances in human bone: concentrations in bones and effects on bone cell differentiation

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