Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution

Abstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this st...

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Autores principales:	Takehiro Hariya, Kazuichi Maruyama, Sunao Sugita, Masayo Takahashi, Shunji Yokokura, Kota Sato, Yasuhiro Tomaru, Norio Shimizu, Toru Nakazawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/153040f092254916a6d0902b901bd821
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spelling	oai:doaj.org-article:153040f092254916a6d0902b901bd8212021-12-02T12:30:51ZMultiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution10.1038/s41598-017-06344-32045-2322https://doaj.org/article/153040f092254916a6d0902b901bd8212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06344-3https://doaj.org/toc/2045-2322Abstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution. The subjects comprised 50 patients who underwent corneal transplantation at Tohoku University Hospital between July 2014 and April 2015. We obtained extracted (recipient) cornea samples in 37 cases, donor cornea samples in 50 cases, and corneal storage solution samples in 50 cases (18 of these 50 samples contained DNA). Herpes simplex virus type 1 DNA was detected in four recipient corneas, Parvovirus B19 DNA was detected in two recipient corneas, Human herpes virus type 6 was detected in two donor corneas, and Aspergillus DNA was detected in one corneal storage solution sample. Thus, mPCR successfully identified pathogenic DNA in corneal tissues and storage solution, suggesting that evaluation with mPCR may improve the ability to predict the risk of infection after corneal transplantation.Takehiro HariyaKazuichi MaruyamaSunao SugitaMasayo TakahashiShunji YokokuraKota SatoYasuhiro TomaruNorio ShimizuToru NakazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
description	Abstract Corneal transplantation is a safe, reliable method of restoring visual acuity in patients with corneal disorders. Although it has a very high success rate, rejection can still occur, especially if the site is infected. Therefore, seeking to find better ways to manage infection risk, this study investigated a new technique, based on multiplex polymerase chain reaction (mPCR), to identify pathogens, including viruses, bacteria, and fungi, in corneal transplantation recipient sites, donor corneas and the donor cornea storage solution. The subjects comprised 50 patients who underwent corneal transplantation at Tohoku University Hospital between July 2014 and April 2015. We obtained extracted (recipient) cornea samples in 37 cases, donor cornea samples in 50 cases, and corneal storage solution samples in 50 cases (18 of these 50 samples contained DNA). Herpes simplex virus type 1 DNA was detected in four recipient corneas, Parvovirus B19 DNA was detected in two recipient corneas, Human herpes virus type 6 was detected in two donor corneas, and Aspergillus DNA was detected in one corneal storage solution sample. Thus, mPCR successfully identified pathogenic DNA in corneal tissues and storage solution, suggesting that evaluation with mPCR may improve the ability to predict the risk of infection after corneal transplantation.
format	article
author	Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa
author_facet	Takehiro Hariya Kazuichi Maruyama Sunao Sugita Masayo Takahashi Shunji Yokokura Kota Sato Yasuhiro Tomaru Norio Shimizu Toru Nakazawa
author_sort	Takehiro Hariya
title	Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
title_short	Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
title_full	Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
title_fullStr	Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
title_full_unstemmed	Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
title_sort	multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/153040f092254916a6d0902b901bd821
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Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution

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