Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles

Gaet’ale (GAL) and Mud’ara (MUP) are two hypersaline ponds located in the Danakil Depression recharged by underground water from the surrounding highlands. These two ponds have different pH, salinity, and show variation in the concentration of many ionic components. Metagenomic analysis concludes th...

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Autores principales:	Lulit Tilahun, Asfawossen Asrat, Gary M. Wessel, Addis Simachew
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	extremophiles Gaet’ale Mud’ara Danakil Depression Ethiopia syntrophic metabolism methanogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/153252e44d814e6699deb35a0ea10e22
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spelling	oai:doaj.org-article:153252e44d814e6699deb35a0ea10e222021-11-25T18:24:17ZPrediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles10.3390/microorganisms91122112076-2607https://doaj.org/article/153252e44d814e6699deb35a0ea10e222021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2211https://doaj.org/toc/2076-2607Gaet’ale (GAL) and Mud’ara (MUP) are two hypersaline ponds located in the Danakil Depression recharged by underground water from the surrounding highlands. These two ponds have different pH, salinity, and show variation in the concentration of many ionic components. Metagenomic analysis concludes that GAL is dominated by bacteria as in the case of the other hypersaline and acidic ponds in the Danakil Depression. However, Archaea dominated the ponds of MUP. In the current study, the application of SEED and KEGG helped to map the ordered steps of specific enzyme catalyzed reaction in converting CO<sub>2</sub> into cell products. We predict that highly efficient and light-independent carbon fixation involving phosphoenolpyruvate carboxylase takes place in MUP. On the contrary, genes encoding enzymes involved in hydrogenotrophic and acetoclastic methanogenesis appeared solely in ponds of GAL, implying the biological source of the hazardous methane gas in that environment. Based on the investigation of the sources of the genes of interest, it is clear that cooperative interactions between members of the two communities and syntrophic metabolism is the main strategy adapted to utilize inorganic carbon as a carbon source in both MUP and GAL. This insight can be used to design biotechnological applications of microbial communities in production of methane biogas or to minimize CO<sub>2</sub> emissions.Lulit TilahunAsfawossen AsratGary M. WesselAddis SimachewMDPI AGarticleextremophilesGaet’aleMud’araDanakil DepressionEthiopia syntrophic metabolismmethanogenesisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2211, p 2211 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extremophiles Gaet’ale Mud’ara Danakil Depression Ethiopia syntrophic metabolism methanogenesis Biology (General) QH301-705.5
spellingShingle	extremophiles Gaet’ale Mud’ara Danakil Depression Ethiopia syntrophic metabolism methanogenesis Biology (General) QH301-705.5 Lulit Tilahun Asfawossen Asrat Gary M. Wessel Addis Simachew Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
description	Gaet’ale (GAL) and Mud’ara (MUP) are two hypersaline ponds located in the Danakil Depression recharged by underground water from the surrounding highlands. These two ponds have different pH, salinity, and show variation in the concentration of many ionic components. Metagenomic analysis concludes that GAL is dominated by bacteria as in the case of the other hypersaline and acidic ponds in the Danakil Depression. However, Archaea dominated the ponds of MUP. In the current study, the application of SEED and KEGG helped to map the ordered steps of specific enzyme catalyzed reaction in converting CO<sub>2</sub> into cell products. We predict that highly efficient and light-independent carbon fixation involving phosphoenolpyruvate carboxylase takes place in MUP. On the contrary, genes encoding enzymes involved in hydrogenotrophic and acetoclastic methanogenesis appeared solely in ponds of GAL, implying the biological source of the hazardous methane gas in that environment. Based on the investigation of the sources of the genes of interest, it is clear that cooperative interactions between members of the two communities and syntrophic metabolism is the main strategy adapted to utilize inorganic carbon as a carbon source in both MUP and GAL. This insight can be used to design biotechnological applications of microbial communities in production of methane biogas or to minimize CO<sub>2</sub> emissions.
format	article
author	Lulit Tilahun Asfawossen Asrat Gary M. Wessel Addis Simachew
author_facet	Lulit Tilahun Asfawossen Asrat Gary M. Wessel Addis Simachew
author_sort	Lulit Tilahun
title	Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
title_short	Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
title_full	Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
title_fullStr	Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
title_full_unstemmed	Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles
title_sort	prediction of genes that function in methanogenesis and co<sub>2</sub> pathways in extremophiles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/153252e44d814e6699deb35a0ea10e22
work_keys_str_mv	AT lulittilahun predictionofgenesthatfunctioninmethanogenesisandcosub2subpathwaysinextremophiles AT asfawossenasrat predictionofgenesthatfunctioninmethanogenesisandcosub2subpathwaysinextremophiles AT garymwessel predictionofgenesthatfunctioninmethanogenesisandcosub2subpathwaysinextremophiles AT addissimachew predictionofgenesthatfunctioninmethanogenesisandcosub2subpathwaysinextremophiles
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Prediction of Genes That Function in Methanogenesis and CO<sub>2</sub> Pathways in Extremophiles

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