Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)

Abstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus R...

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Autores principales:	Anna C. Fagre, Juliette Lewis, Megan R. Miller, Eric C. Mossel, Julius J. Lutwama, Luke Nyakarahuka, Teddy Nakayiki, Robert Kityo, Betty Nalikka, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Brian Foy, Tony Schountz, John Anderson, Rebekah C. Kading
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/153a44505c124faebb4f55fc624224a0
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spelling	oai:doaj.org-article:153a44505c124faebb4f55fc624224a02021-12-02T14:27:56ZSubgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)10.1038/s41598-021-87816-52045-2322https://doaj.org/article/153a44505c124faebb4f55fc624224a02021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87816-5https://doaj.org/toc/2045-2322Abstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3′ untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats.Anna C. FagreJuliette LewisMegan R. MillerEric C. MosselJulius J. LutwamaLuke NyakarahukaTeddy NakayikiRobert KityoBetty NalikkaJonathan S. TownerBrian R. AmmanTara K. SealyBrian FoyTony SchountzJohn AndersonRebekah C. KadingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
description	Abstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3′ untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats.
format	article
author	Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading
author_facet	Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading
author_sort	Anna C. Fagre
title	Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
title_short	Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
title_full	Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
title_fullStr	Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
title_full_unstemmed	Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
title_sort	subgenomic flavivirus rna (sfrna) associated with asian lineage zika virus identified in three species of ugandan bats (family pteropodidae)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/153a44505c124faebb4f55fc624224a0
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Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)

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