Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.

An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is ba...

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Autores principales:	Christine Vignon, Christelle Debeissat, Marie-Thérèse Georget, Didier Bouscary, Emmanuel Gyan, Philippe Rosset, Olivier Herault
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15449e40bade46b0b85c696e0fc59e71
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spelling	oai:doaj.org-article:15449e40bade46b0b85c696e0fc59e712021-11-18T09:02:05ZFlow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.1932-620310.1371/journal.pone.0068425https://doaj.org/article/15449e40bade46b0b85c696e0fc59e712013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935867/?tool=EBIhttps://doaj.org/toc/1932-6203An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.Christine VignonChristelle DebeissatMarie-Thérèse GeorgetDidier BouscaryEmmanuel GyanPhilippe RossetOlivier HeraultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68425 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christine Vignon Christelle Debeissat Marie-Thérèse Georget Didier Bouscary Emmanuel Gyan Philippe Rosset Olivier Herault Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
description	An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.
format	article
author	Christine Vignon Christelle Debeissat Marie-Thérèse Georget Didier Bouscary Emmanuel Gyan Philippe Rosset Olivier Herault
author_facet	Christine Vignon Christelle Debeissat Marie-Thérèse Georget Didier Bouscary Emmanuel Gyan Philippe Rosset Olivier Herault
author_sort	Christine Vignon
title	Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
title_short	Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
title_full	Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
title_fullStr	Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
title_full_unstemmed	Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
title_sort	flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/15449e40bade46b0b85c696e0fc59e71
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Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.

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