Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum

Abstract Sugarcane is the most important sugar and biofuel crop. MADS-box genes encode transcription factors that are involved in developmental control and signal transduction in plants. Systematic analyses of MADS-box genes have been reported in many plant species, but its identification and charac...

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Autores principales:	Mahpara Fatima, Xiaodan Zhang, Jishan Lin, Ping Zhou, Dong Zhou, Ray Ming
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/154544d0227b4e8a98e4a939df1ca05c
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spelling	oai:doaj.org-article:154544d0227b4e8a98e4a939df1ca05c2021-12-02T11:40:41ZExpression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum10.1038/s41598-020-77375-62045-2322https://doaj.org/article/154544d0227b4e8a98e4a939df1ca05c2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77375-6https://doaj.org/toc/2045-2322Abstract Sugarcane is the most important sugar and biofuel crop. MADS-box genes encode transcription factors that are involved in developmental control and signal transduction in plants. Systematic analyses of MADS-box genes have been reported in many plant species, but its identification and characterization were not possible until a reference genome of autotetraploid wild type sugarcane specie, Saccharum spontaneum is available recently. We identified 182 MADS-box sequences in the S. spontaneum genome, which were annotated into 63 genes, including 6 (9.5%) genes with four alleles, 21 (33.3%) with three, 29 (46%) with two, 7 (11.1%) with one allele. Paralogs (tandem duplication and disperse duplicated) were also identified and characterized. These MADS-box genes were divided into two groups; Type-I (21 Mα, 4 Mβ, 4 Mγ) and Type-II (32 MIKCc, 2 MIKC*) through phylogenetic analysis with orthologs in Arabidopsis and sorghum. Structural diversity and distribution of motifs were studied in detail. Chromosomal localizations revealed that S. spontaneum MADS-box genes were randomly distributed across eight homologous chromosome groups. The expression profiles of these MADS-box genes were analyzed in leaves, roots, stem sections and after hormones treatment. Important alleles based on promoter analysis and expression variations were dissected. qRT-PCR analysis was performed to verify the expression pattern of pivotal S. spontaneum MADS-box genes and suggested that flower timing genes (SOC1 and SVP) may regulate vegetative development.Mahpara FatimaXiaodan ZhangJishan LinPing ZhouDong ZhouRay MingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahpara Fatima Xiaodan Zhang Jishan Lin Ping Zhou Dong Zhou Ray Ming Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
description	Abstract Sugarcane is the most important sugar and biofuel crop. MADS-box genes encode transcription factors that are involved in developmental control and signal transduction in plants. Systematic analyses of MADS-box genes have been reported in many plant species, but its identification and characterization were not possible until a reference genome of autotetraploid wild type sugarcane specie, Saccharum spontaneum is available recently. We identified 182 MADS-box sequences in the S. spontaneum genome, which were annotated into 63 genes, including 6 (9.5%) genes with four alleles, 21 (33.3%) with three, 29 (46%) with two, 7 (11.1%) with one allele. Paralogs (tandem duplication and disperse duplicated) were also identified and characterized. These MADS-box genes were divided into two groups; Type-I (21 Mα, 4 Mβ, 4 Mγ) and Type-II (32 MIKCc, 2 MIKC*) through phylogenetic analysis with orthologs in Arabidopsis and sorghum. Structural diversity and distribution of motifs were studied in detail. Chromosomal localizations revealed that S. spontaneum MADS-box genes were randomly distributed across eight homologous chromosome groups. The expression profiles of these MADS-box genes were analyzed in leaves, roots, stem sections and after hormones treatment. Important alleles based on promoter analysis and expression variations were dissected. qRT-PCR analysis was performed to verify the expression pattern of pivotal S. spontaneum MADS-box genes and suggested that flower timing genes (SOC1 and SVP) may regulate vegetative development.
format	article
author	Mahpara Fatima Xiaodan Zhang Jishan Lin Ping Zhou Dong Zhou Ray Ming
author_facet	Mahpara Fatima Xiaodan Zhang Jishan Lin Ping Zhou Dong Zhou Ray Ming
author_sort	Mahpara Fatima
title	Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
title_short	Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
title_full	Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
title_fullStr	Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
title_full_unstemmed	Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum
title_sort	expression profiling of mads-box gene family revealed its role in vegetative development and stem ripening in s. spontaneum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/154544d0227b4e8a98e4a939df1ca05c
work_keys_str_mv	AT mahparafatima expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum AT xiaodanzhang expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum AT jishanlin expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum AT pingzhou expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum AT dongzhou expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum AT rayming expressionprofilingofmadsboxgenefamilyrevealeditsroleinvegetativedevelopmentandstemripeninginsspontaneum
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Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum

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