Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.

<h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions...

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Autores principales:	Estelle Marion, Soizic Prado, Camille Cano, Jérémie Babonneau, Sarah Ghamrawi, Laurent Marsollier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15464e47bb414a3ca069da780b8ddfc0
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spelling	oai:doaj.org-article:15464e47bb414a3ca069da780b8ddfc02021-11-18T07:22:22ZPhotodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.1932-620310.1371/journal.pone.0033600https://doaj.org/article/15464e47bb414a3ca069da780b8ddfc02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514607/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions even at early stages, mycolactones are promising candidates for the development of a diagnostic tool for M. ulcerans infection. Stability of purified mycolactones towards light and heat has not yet been investigated, despite the importance of such parameters in the selection of strategies for a diagnosis tool development. In this context, the effects of UV, light and temperature on mycolactone stability and biological activity were studied.<h4>Methodology/principal findings</h4>To investigate the effect of these physical parameters, mycolactones were exposed to different wavelengths in several solvents and temperatures. Structural changes and biological activity were monitored. Whilst high temperature had no effect on mycolactones, UV irradiation (UV-A, UV-B and UV-C) and sunlight exposure caused a considerable degradation, as revealed by LC-MS and NMR analysis, correlated with a loss of biological activity. Moreover, effect of UVs on mycolactone caused a photodegradation rather than a phototransformation due to the identification of degradation product.<h4>Conclusion/significance</h4>This study demonstrates the high sensitivity of mycolactones to UVs as such it defines instructions for storage and handling.Estelle MarionSoizic PradoCamille CanoJérémie BabonneauSarah GhamrawiLaurent MarsollierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e33600 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
description	<h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions even at early stages, mycolactones are promising candidates for the development of a diagnostic tool for M. ulcerans infection. Stability of purified mycolactones towards light and heat has not yet been investigated, despite the importance of such parameters in the selection of strategies for a diagnosis tool development. In this context, the effects of UV, light and temperature on mycolactone stability and biological activity were studied.<h4>Methodology/principal findings</h4>To investigate the effect of these physical parameters, mycolactones were exposed to different wavelengths in several solvents and temperatures. Structural changes and biological activity were monitored. Whilst high temperature had no effect on mycolactones, UV irradiation (UV-A, UV-B and UV-C) and sunlight exposure caused a considerable degradation, as revealed by LC-MS and NMR analysis, correlated with a loss of biological activity. Moreover, effect of UVs on mycolactone caused a photodegradation rather than a phototransformation due to the identification of degradation product.<h4>Conclusion/significance</h4>This study demonstrates the high sensitivity of mycolactones to UVs as such it defines instructions for storage and handling.
format	article
author	Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier
author_facet	Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier
author_sort	Estelle Marion
title	Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
title_short	Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
title_full	Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
title_fullStr	Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
title_full_unstemmed	Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
title_sort	photodegradation of the mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/15464e47bb414a3ca069da780b8ddfc0
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Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.

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