Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice

Abstract Atherosclerotic plaques are characterized by an accumulation and subsequent oxidation of LDL, resulting in adaptive immune responses against formed or exposed neoepitopes of the LDL particle. Autoantibodies against native p210, the 3136–3155 amino acid sequence of the LDL protein apolipopro...

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Autores principales:	Pontus Dunér, Ingrid Yao Mattisson, Per Fogelstrand, Lars Glise, Stacey Ruiz, Christopher Farina, Jan Borén, Jan Nilsson, Eva Bengtsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/155b069540a744b0905d0437eeb14b5a
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spelling	oai:doaj.org-article:155b069540a744b0905d0437eeb14b5a2021-12-02T16:55:54ZAntibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice10.1038/s41598-021-88430-12045-2322https://doaj.org/article/155b069540a744b0905d0437eeb14b5a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88430-1https://doaj.org/toc/2045-2322Abstract Atherosclerotic plaques are characterized by an accumulation and subsequent oxidation of LDL, resulting in adaptive immune responses against formed or exposed neoepitopes of the LDL particle. Autoantibodies against native p210, the 3136–3155 amino acid sequence of the LDL protein apolipoprotein B-100 (apoB100) are common in humans and have been associated with less severe atherosclerosis and decreased risk for cardiovascular events in clinical studies. However, whether apoB100 native p210 autoantibodies play a functional role in atherosclerosis is not known. In the present study we immunized apoE-/- mice with p210-PADRE peptide to induce an antibody response against native p210. We also injected mice with murine monoclonal IgG against native p210. Control groups were immunized with PADRE peptide alone or with control murine monoclonal IgG. Immunization with p210-PADRE induced an IgG1 antibody response against p210 that was associated with reduced atherosclerotic plaque formation in the aorta and reduced MDA-LDL content in the lesions. Treatment with monoclonal p210 IgG produced a similar reduction in atherosclerosis as immunization with p210-PADRE. Our findings support an atheroprotective role of antibodies against the apoB100 native p210 and suggest that vaccines that induce the expression of native p210 IgG represent a potential therapeutic strategy for lowering cardiovascular risk.Pontus DunérIngrid Yao MattissonPer FogelstrandLars GliseStacey RuizChristopher FarinaJan BorénJan NilssonEva BengtssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pontus Dunér Ingrid Yao Mattisson Per Fogelstrand Lars Glise Stacey Ruiz Christopher Farina Jan Borén Jan Nilsson Eva Bengtsson Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
description	Abstract Atherosclerotic plaques are characterized by an accumulation and subsequent oxidation of LDL, resulting in adaptive immune responses against formed or exposed neoepitopes of the LDL particle. Autoantibodies against native p210, the 3136–3155 amino acid sequence of the LDL protein apolipoprotein B-100 (apoB100) are common in humans and have been associated with less severe atherosclerosis and decreased risk for cardiovascular events in clinical studies. However, whether apoB100 native p210 autoantibodies play a functional role in atherosclerosis is not known. In the present study we immunized apoE-/- mice with p210-PADRE peptide to induce an antibody response against native p210. We also injected mice with murine monoclonal IgG against native p210. Control groups were immunized with PADRE peptide alone or with control murine monoclonal IgG. Immunization with p210-PADRE induced an IgG1 antibody response against p210 that was associated with reduced atherosclerotic plaque formation in the aorta and reduced MDA-LDL content in the lesions. Treatment with monoclonal p210 IgG produced a similar reduction in atherosclerosis as immunization with p210-PADRE. Our findings support an atheroprotective role of antibodies against the apoB100 native p210 and suggest that vaccines that induce the expression of native p210 IgG represent a potential therapeutic strategy for lowering cardiovascular risk.
format	article
author	Pontus Dunér Ingrid Yao Mattisson Per Fogelstrand Lars Glise Stacey Ruiz Christopher Farina Jan Borén Jan Nilsson Eva Bengtsson
author_facet	Pontus Dunér Ingrid Yao Mattisson Per Fogelstrand Lars Glise Stacey Ruiz Christopher Farina Jan Borén Jan Nilsson Eva Bengtsson
author_sort	Pontus Dunér
title	Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
title_short	Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
title_full	Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
title_fullStr	Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
title_full_unstemmed	Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice
title_sort	antibodies against apob100 peptide 210 inhibit atherosclerosis in apoe-/- mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/155b069540a744b0905d0437eeb14b5a
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Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/- mice

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