A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA
Dimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles in global sulfur and nutrient cycling. Diverse DMSP lyases in some algae, bacteria, and fungi cleave DMSP to yield gaseous dimethyl sulfide (DMS), an infochemical with im...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:155c9dc9f49743a7b384a5b4174ce9402021-11-25T14:34:59ZA novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA10.7554/eLife.640452050-084Xe64045https://doaj.org/article/155c9dc9f49743a7b384a5b4174ce9402021-05-01T00:00:00Zhttps://elifesciences.org/articles/64045https://doaj.org/toc/2050-084XDimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles in global sulfur and nutrient cycling. Diverse DMSP lyases in some algae, bacteria, and fungi cleave DMSP to yield gaseous dimethyl sulfide (DMS), an infochemical with important roles in atmospheric chemistry. Here, we identified a novel ATP-dependent DMSP lyase, DddX. DddX belongs to the acyl-CoA synthetase superfamily and is distinct from the eight other known DMSP lyases. DddX catalyses the conversion of DMSP to DMS via a two-step reaction: the ligation of DMSP with CoA to form the intermediate DMSP-CoA, which is then cleaved to DMS and acryloyl-CoA. The novel catalytic mechanism was elucidated by structural and biochemical analyses. DddX is found in several Alphaproteobacteria, Gammaproteobacteria, and Firmicutes, suggesting that this new DMSP lyase may play an overlooked role in DMSP/DMS cycles.Chun-Yang LiXiu-Juan WangXiu-Lan ChenQi ShengShan ZhangPeng WangMussa QuareshyBranko RihtmanXuan ShaoChao GaoFuchuan LiShengying LiWeipeng ZhangXiao-Hua ZhangGui-Peng YangJonathan D ToddYin ChenYu-Zhong ZhangeLife Sciences Publications Ltdarticledmsp cleavage pathwaydms generationATP-dependent DMSP lyaseglobal sulfur cyclemarine bacteriaMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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DOAJ |
| language |
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| topic |
dmsp cleavage pathway dms generation ATP-dependent DMSP lyase global sulfur cycle marine bacteria Medicine R Science Q Biology (General) QH301-705.5 |
| spellingShingle |
dmsp cleavage pathway dms generation ATP-dependent DMSP lyase global sulfur cycle marine bacteria Medicine R Science Q Biology (General) QH301-705.5 Chun-Yang Li Xiu-Juan Wang Xiu-Lan Chen Qi Sheng Shan Zhang Peng Wang Mussa Quareshy Branko Rihtman Xuan Shao Chao Gao Fuchuan Li Shengying Li Weipeng Zhang Xiao-Hua Zhang Gui-Peng Yang Jonathan D Todd Yin Chen Yu-Zhong Zhang A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| description |
Dimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles in global sulfur and nutrient cycling. Diverse DMSP lyases in some algae, bacteria, and fungi cleave DMSP to yield gaseous dimethyl sulfide (DMS), an infochemical with important roles in atmospheric chemistry. Here, we identified a novel ATP-dependent DMSP lyase, DddX. DddX belongs to the acyl-CoA synthetase superfamily and is distinct from the eight other known DMSP lyases. DddX catalyses the conversion of DMSP to DMS via a two-step reaction: the ligation of DMSP with CoA to form the intermediate DMSP-CoA, which is then cleaved to DMS and acryloyl-CoA. The novel catalytic mechanism was elucidated by structural and biochemical analyses. DddX is found in several Alphaproteobacteria, Gammaproteobacteria, and Firmicutes, suggesting that this new DMSP lyase may play an overlooked role in DMSP/DMS cycles. |
| format |
article |
| author |
Chun-Yang Li Xiu-Juan Wang Xiu-Lan Chen Qi Sheng Shan Zhang Peng Wang Mussa Quareshy Branko Rihtman Xuan Shao Chao Gao Fuchuan Li Shengying Li Weipeng Zhang Xiao-Hua Zhang Gui-Peng Yang Jonathan D Todd Yin Chen Yu-Zhong Zhang |
| author_facet |
Chun-Yang Li Xiu-Juan Wang Xiu-Lan Chen Qi Sheng Shan Zhang Peng Wang Mussa Quareshy Branko Rihtman Xuan Shao Chao Gao Fuchuan Li Shengying Li Weipeng Zhang Xiao-Hua Zhang Gui-Peng Yang Jonathan D Todd Yin Chen Yu-Zhong Zhang |
| author_sort |
Chun-Yang Li |
| title |
A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| title_short |
A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| title_full |
A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| title_fullStr |
A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| title_full_unstemmed |
A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA |
| title_sort |
novel atp dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-coa |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/155c9dc9f49743a7b384a5b4174ce940 |
| work_keys_str_mv |
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1718413363910803456 |