4Pi-RESOLFT nanoscopy

The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.

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Detalles Bibliográficos
Autores principales: Ulrike Böhm, Stefan W. Hell, Roman Schmidt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/15612915e17c48a98f956eb5c66c2d2e
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Descripción
Sumario:The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.