4Pi-RESOLFT nanoscopy

4Pi-RESOLFT nanoscopy

The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.

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Autores principales: Ulrike Böhm, Stefan W. Hell, Roman Schmidt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/15612915e17c48a98f956eb5c66c2d2e
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spelling oai:doaj.org-article:15612915e17c48a98f956eb5c66c2d2e2021-12-02T14:40:12Z4Pi-RESOLFT nanoscopy10.1038/ncomms105042041-1723https://doaj.org/article/15612915e17c48a98f956eb5c66c2d2e2016-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms10504https://doaj.org/toc/2041-1723The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.Ulrike BöhmStefan W. HellRoman SchmidtNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ulrike Böhm
Stefan W. Hell
Roman Schmidt
4Pi-RESOLFT nanoscopy
description The ability to discriminate objects along the optic axis is a benchmark for three-dimensional imaging techniques. Here, the authors combine metastable-state switching and opposing objective lenses to suppress out-of-focus background and record three-dimensional nanoscale images of living cells.
format article
author Ulrike Böhm
Stefan W. Hell
Roman Schmidt
author_facet Ulrike Böhm
Stefan W. Hell
Roman Schmidt
author_sort Ulrike Böhm
title 4Pi-RESOLFT nanoscopy
title_short 4Pi-RESOLFT nanoscopy
title_full 4Pi-RESOLFT nanoscopy
title_fullStr 4Pi-RESOLFT nanoscopy
title_full_unstemmed 4Pi-RESOLFT nanoscopy
title_sort 4pi-resolft nanoscopy
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/15612915e17c48a98f956eb5c66c2d2e
work_keys_str_mv AT ulrikebohm 4piresolftnanoscopy
AT stefanwhell 4piresolftnanoscopy
AT romanschmidt 4piresolftnanoscopy
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