Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities

Abstract Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associa...

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Autores principales:	Anna M. Alessi, Susannah M. Bird, Joseph P. Bennett, Nicola C. Oates, Yi Li, Adam A. Dowle, Igor Polikarpov, J Peter W. Young, Simon J. McQueen-Mason, Neil C. Bruce
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/156206cfaeea45aabc9144b00d800446
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spelling	oai:doaj.org-article:156206cfaeea45aabc9144b00d8004462021-12-02T15:04:53ZRevealing the insoluble metasecretome of lignocellulose-degrading microbial communities10.1038/s41598-017-02506-52045-2322https://doaj.org/article/156206cfaeea45aabc9144b00d8004462017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02506-5https://doaj.org/toc/2045-2322Abstract Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing intracellular proteins that contaminate the metasecretome. Here we describe a technique for targeting the extracellular proteome, which was used to compare the metasecretome and meta-surface-proteome of two lignocellulose-degrading communities grown on wheat straw and rice straw. A combination of mass spectrometry-based proteomics coupled with metatranscriptomics enabled the identification of a unique secretome pool from these lignocellulose-degrading communities. This method enabled us to efficiently discriminate the extracellular proteins from the intracellular proteins by improving detection of actively secreted and transmembrane proteins. In addition to the expected carbohydrate active enzymes, our new method reveals a large number of unknown proteins, supporting the notion that there are major gaps in our understanding of how microbial communities degrade lignocellulosic substrates.Anna M. AlessiSusannah M. BirdJoseph P. BennettNicola C. OatesYi LiAdam A. DowleIgor PolikarpovJ Peter W. YoungSimon J. McQueen-MasonNeil C. BruceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna M. Alessi Susannah M. Bird Joseph P. Bennett Nicola C. Oates Yi Li Adam A. Dowle Igor Polikarpov J Peter W. Young Simon J. McQueen-Mason Neil C. Bruce Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
description	Abstract Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing intracellular proteins that contaminate the metasecretome. Here we describe a technique for targeting the extracellular proteome, which was used to compare the metasecretome and meta-surface-proteome of two lignocellulose-degrading communities grown on wheat straw and rice straw. A combination of mass spectrometry-based proteomics coupled with metatranscriptomics enabled the identification of a unique secretome pool from these lignocellulose-degrading communities. This method enabled us to efficiently discriminate the extracellular proteins from the intracellular proteins by improving detection of actively secreted and transmembrane proteins. In addition to the expected carbohydrate active enzymes, our new method reveals a large number of unknown proteins, supporting the notion that there are major gaps in our understanding of how microbial communities degrade lignocellulosic substrates.
format	article
author	Anna M. Alessi Susannah M. Bird Joseph P. Bennett Nicola C. Oates Yi Li Adam A. Dowle Igor Polikarpov J Peter W. Young Simon J. McQueen-Mason Neil C. Bruce
author_facet	Anna M. Alessi Susannah M. Bird Joseph P. Bennett Nicola C. Oates Yi Li Adam A. Dowle Igor Polikarpov J Peter W. Young Simon J. McQueen-Mason Neil C. Bruce
author_sort	Anna M. Alessi
title	Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
title_short	Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
title_full	Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
title_fullStr	Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
title_full_unstemmed	Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
title_sort	revealing the insoluble metasecretome of lignocellulose-degrading microbial communities
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/156206cfaeea45aabc9144b00d800446
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Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities

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