Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications

Alaa J Hassiba,1 Mohamed E El Zowalaty,2 Thomas J Webster,3–5 Aboubakr M Abdullah,6 Gheyath K Nasrallah,7 Khalil Abdelrazek Khalil,8 Adriaan S Luyt,6 Ahmed A Elzatahry1 1Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha, Qatar; 2School of Healt...

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Autores principales:	Hassiba AJ, El Zowalaty ME, Webster TJ, Abdullah AM, Nasrallah GK, Khalil KA, Luyt AS, Elzatahry AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Nanomaterials wound dressing nanofibers electrospinning biomedical antimicrobial activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1568640d164940e885fea9ee01b6371b
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spelling	oai:doaj.org-article:1568640d164940e885fea9ee01b6371b2021-12-02T02:31:36ZSynthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications1178-2013https://doaj.org/article/1568640d164940e885fea9ee01b6371b2017-03-01T00:00:00Zhttps://www.dovepress.com/synthesis-characterization-and-antimicrobial-properties-of-novel-doubl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alaa J Hassiba,1 Mohamed E El Zowalaty,2 Thomas J Webster,3–5 Aboubakr M Abdullah,6 Gheyath K Nasrallah,7 Khalil Abdelrazek Khalil,8 Adriaan S Luyt,6 Ahmed A Elzatahry1 1Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha, Qatar; 2School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; 3Department of Chemical Engineering, 4Department of Bioengineering, Northeastern University, Boston, MA, USA; 5Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia; 6Center for Advanced Materials, 7Department of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar University, Doha, Qatar; 8Department of Mechanical Engineering, College of Engineering, University of Sharjah, Sharjah, United Arab Emirates Abstract: Herein, novel hybrid nanomaterials were developed for wound dressing applications with antimicrobial properties. Electrospinning was used to fabricate a double layer nanocomposite nanofibrous mat consisting of an upper layer of poly(vinyl alcohol) and chitosan loaded with silver nanoparticles (AgNPs) and a lower layer of polyethylene oxide (PEO) or polyvinylpyrrolidone (PVP) nanofibers loaded with chlorhexidine (as an antiseptic). The top layer containing AgNPs, whose purpose was to protect the wound site against environmental germ invasion, was prepared by reducing silver nitrate to its nanoparticulate form through interaction with chitosan. The lower layer, which would be in direct contact with the injured site, contained the antibiotic drug needed to avoid wound infections which would otherwise interfere with the healing process. Initially, the upper layer was electrospun, followed sequentially by electrospinning the second layer, creating a bilayer nanofibrous mat. The morphology of the nanofibrous mats was studied by scanning electron microscopy and transmission electron microscopy, showing successful nanofiber production. X-ray diffraction confirmed the reduction of silver nitrate to AgNPs. Fourier transform infrared spectroscopy showed a successful incorporation of the material used in the produced nanofibrous mats. Thermal studies carried out by thermogravimetric analysis indicated that the PVP–drug-loaded layer had the highest thermal stability in comparison to other fabricated nanofibrous mats. Antimicrobial activities of the as-synthesized nanofibrous mats against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans were determined using disk diffusion method. The results indicated that the PEO–drug-loaded mat had the highest antibacterial activity, warranting further attention for numerous wound-healing applications. Keywords: nanomaterials, wound dressing, nanofibers, electrospinning, biomedical, antimicrobial, activityHassiba AJEl Zowalaty MEWebster TJAbdullah AMNasrallah GKKhalil KALuyt ASElzatahry AADove Medical PressarticleNanomaterialswound dressingnanofiberselectrospinningbiomedicalantimicrobialactivityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2205-2213 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanomaterials wound dressing nanofibers electrospinning biomedical antimicrobial activity Medicine (General) R5-920
spellingShingle	Nanomaterials wound dressing nanofibers electrospinning biomedical antimicrobial activity Medicine (General) R5-920 Hassiba AJ El Zowalaty ME Webster TJ Abdullah AM Nasrallah GK Khalil KA Luyt AS Elzatahry AA Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
description	Alaa J Hassiba,1 Mohamed E El Zowalaty,2 Thomas J Webster,3–5 Aboubakr M Abdullah,6 Gheyath K Nasrallah,7 Khalil Abdelrazek Khalil,8 Adriaan S Luyt,6 Ahmed A Elzatahry1 1Materials Science and Technology Program, College of Arts and Sciences, Qatar University, Doha, Qatar; 2School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; 3Department of Chemical Engineering, 4Department of Bioengineering, Northeastern University, Boston, MA, USA; 5Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia; 6Center for Advanced Materials, 7Department of Biomedical Science, College of Health Sciences, Biomedical Research Center, Qatar University, Doha, Qatar; 8Department of Mechanical Engineering, College of Engineering, University of Sharjah, Sharjah, United Arab Emirates Abstract: Herein, novel hybrid nanomaterials were developed for wound dressing applications with antimicrobial properties. Electrospinning was used to fabricate a double layer nanocomposite nanofibrous mat consisting of an upper layer of poly(vinyl alcohol) and chitosan loaded with silver nanoparticles (AgNPs) and a lower layer of polyethylene oxide (PEO) or polyvinylpyrrolidone (PVP) nanofibers loaded with chlorhexidine (as an antiseptic). The top layer containing AgNPs, whose purpose was to protect the wound site against environmental germ invasion, was prepared by reducing silver nitrate to its nanoparticulate form through interaction with chitosan. The lower layer, which would be in direct contact with the injured site, contained the antibiotic drug needed to avoid wound infections which would otherwise interfere with the healing process. Initially, the upper layer was electrospun, followed sequentially by electrospinning the second layer, creating a bilayer nanofibrous mat. The morphology of the nanofibrous mats was studied by scanning electron microscopy and transmission electron microscopy, showing successful nanofiber production. X-ray diffraction confirmed the reduction of silver nitrate to AgNPs. Fourier transform infrared spectroscopy showed a successful incorporation of the material used in the produced nanofibrous mats. Thermal studies carried out by thermogravimetric analysis indicated that the PVP–drug-loaded layer had the highest thermal stability in comparison to other fabricated nanofibrous mats. Antimicrobial activities of the as-synthesized nanofibrous mats against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans were determined using disk diffusion method. The results indicated that the PEO–drug-loaded mat had the highest antibacterial activity, warranting further attention for numerous wound-healing applications. Keywords: nanomaterials, wound dressing, nanofibers, electrospinning, biomedical, antimicrobial, activity
format	article
author	Hassiba AJ El Zowalaty ME Webster TJ Abdullah AM Nasrallah GK Khalil KA Luyt AS Elzatahry AA
author_facet	Hassiba AJ El Zowalaty ME Webster TJ Abdullah AM Nasrallah GK Khalil KA Luyt AS Elzatahry AA
author_sort	Hassiba AJ
title	Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
title_short	Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
title_full	Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
title_fullStr	Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
title_full_unstemmed	Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
title_sort	synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/1568640d164940e885fea9ee01b6371b
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Synthesis, characterization, and antimicrobial properties of novel double layer nanocomposite electrospun fibers for wound dressing applications

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