MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

Abstract While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung epithelial cells. Primary human bronchial...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Siva Kumar Solleti, Soumyaroop Bhattacharya, Ausaf Ahmad, Qian Wang, Jared Mereness, Tirumalai Rangasamy, Thomas J. Mariani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1568ab08e650429ea4c1c66de6c2b8d9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1568ab08e650429ea4c1c66de6c2b8d9
record_format	dspace
spelling	oai:doaj.org-article:1568ab08e650429ea4c1c66de6c2b8d92021-12-02T12:32:39ZMicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells10.1038/s41598-017-01167-82045-2322https://doaj.org/article/1568ab08e650429ea4c1c66de6c2b8d92017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01167-8https://doaj.org/toc/2045-2322Abstract While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung epithelial cells. Primary human bronchial epithelial (NHBE) cells differentiated at air-liquid interface were exposed to eCig liquid. Exposure of NHBE to any eCig liquid resulted in the induction of oxidative stress-response genes including GCLM, GCLC, GPX2, NQO1 and HO-1. Vaporization of, and/or the presence of nicotine in, eCig liquid was associated with a greater response. We identified 578 miRNAs dysregulated by eCig exposure in NHBE, and 125 miRNA affected by vaporization of eCig liquid. Nicotine containing eCig vapor displayed the most profound effects upon miRNA expression. We selected 8 miRNAs (29A, 140, 126, 374A, 26A-2, 147B, 941 and 589) for further study. We validated increased expression of multiple miRNAs, including miR126, following eCig exposure. We also found significant reduction in the expression of two miR126 target genes, MYC and MRGPRX3, following exposure. These data demonstrated that eCig exposure has profound effects upon gene expression in human lung epithelial cells, some of which are epigenetically programmed at the level of miRNA regulation.Siva Kumar SolletiSoumyaroop BhattacharyaAusaf AhmadQian WangJared MerenessTirumalai RangasamyThomas J. MarianiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siva Kumar Solleti Soumyaroop Bhattacharya Ausaf Ahmad Qian Wang Jared Mereness Tirumalai Rangasamy Thomas J. Mariani MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
description	Abstract While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung epithelial cells. Primary human bronchial epithelial (NHBE) cells differentiated at air-liquid interface were exposed to eCig liquid. Exposure of NHBE to any eCig liquid resulted in the induction of oxidative stress-response genes including GCLM, GCLC, GPX2, NQO1 and HO-1. Vaporization of, and/or the presence of nicotine in, eCig liquid was associated with a greater response. We identified 578 miRNAs dysregulated by eCig exposure in NHBE, and 125 miRNA affected by vaporization of eCig liquid. Nicotine containing eCig vapor displayed the most profound effects upon miRNA expression. We selected 8 miRNAs (29A, 140, 126, 374A, 26A-2, 147B, 941 and 589) for further study. We validated increased expression of multiple miRNAs, including miR126, following eCig exposure. We also found significant reduction in the expression of two miR126 target genes, MYC and MRGPRX3, following exposure. These data demonstrated that eCig exposure has profound effects upon gene expression in human lung epithelial cells, some of which are epigenetically programmed at the level of miRNA regulation.
format	article
author	Siva Kumar Solleti Soumyaroop Bhattacharya Ausaf Ahmad Qian Wang Jared Mereness Tirumalai Rangasamy Thomas J. Mariani
author_facet	Siva Kumar Solleti Soumyaroop Bhattacharya Ausaf Ahmad Qian Wang Jared Mereness Tirumalai Rangasamy Thomas J. Mariani
author_sort	Siva Kumar Solleti
title	MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
title_short	MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
title_full	MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
title_fullStr	MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
title_full_unstemmed	MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
title_sort	microrna expression profiling defines the impact of electronic cigarettes on human airway epithelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1568ab08e650429ea4c1c66de6c2b8d9
work_keys_str_mv	AT sivakumarsolleti micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT soumyaroopbhattacharya micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT ausafahmad micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT qianwang micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT jaredmereness micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT tirumalairangasamy micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells AT thomasjmariani micrornaexpressionprofilingdefinestheimpactofelectroniccigarettesonhumanairwayepithelialcells
_version_	1718393962881875968

MicroRNA expression profiling defines the impact of electronic cigarettes on human airway epithelial cells

Ejemplares similares