High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey

<i>Diplocyclos palmatus</i> (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin...

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Autores principales:	Anamica Upadhyay, Anwar Shahzad, Zishan Ahmad, Abdulrahman A. Alatar, Gea Guerriero, Mohammad Faisal
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	micropropagation genetic fidelity scanning electron microscopy gas chromatography-mass spectrometry Agriculture S
Acceso en línea:	https://doaj.org/article/156a4e7873d041c386bfc4e701e5c8c7
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spelling	oai:doaj.org-article:156a4e7873d041c386bfc4e701e5c8c72021-11-25T16:04:48ZHigh Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey10.3390/agronomy111121642073-4395https://doaj.org/article/156a4e7873d041c386bfc4e701e5c8c72021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2164https://doaj.org/toc/2073-4395<i>Diplocyclos palmatus</i> (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in <i>D. palmatus</i>.Anamica UpadhyayAnwar ShahzadZishan AhmadAbdulrahman A. AlatarGea GuerrieroMohammad FaisalMDPI AGarticlemicropropagationgenetic fidelityscanning electron microscopygas chromatography-mass spectrometryAgricultureSENAgronomy, Vol 11, Iss 2164, p 2164 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	micropropagation genetic fidelity scanning electron microscopy gas chromatography-mass spectrometry Agriculture S
spellingShingle	micropropagation genetic fidelity scanning electron microscopy gas chromatography-mass spectrometry Agriculture S Anamica Upadhyay Anwar Shahzad Zishan Ahmad Abdulrahman A. Alatar Gea Guerriero Mohammad Faisal High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
description	<i>Diplocyclos palmatus</i> (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in <i>D. palmatus</i>.
format	article
author	Anamica Upadhyay Anwar Shahzad Zishan Ahmad Abdulrahman A. Alatar Gea Guerriero Mohammad Faisal
author_facet	Anamica Upadhyay Anwar Shahzad Zishan Ahmad Abdulrahman A. Alatar Gea Guerriero Mohammad Faisal
author_sort	Anamica Upadhyay
title	High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
title_short	High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
title_full	High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
title_fullStr	High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
title_full_unstemmed	High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey
title_sort	high frequency direct organogenesis, genetic homogeneity, chemical characterization and leaf ultra-structural study of regenerants in <i>diplocyclos palmatus</i> (l.) c. jeffrey
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/156a4e7873d041c386bfc4e701e5c8c7
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High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in <i>Diplocyclos palmatus</i> (L.) C. Jeffrey

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