Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence

Abstract. Arif MF, Aristya GR, Kasiamdari RS. 2019. Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence. Biodiversitas 20: 1721-1728. Banyuroto Village, Magelang, Central Java is the center of strawberry (Fragaria spp.) developm...

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Autores principales:	MUHAMMAD FAUZI ARIF, GANIES RIZA ARISTYA, RINA SRI KASIAMDARI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	caps, fragaria, genetic variation, molecular markers, strawberry, restriction enzyme Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/156b40077d344909b9ce5858c45bcc13
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spelling	oai:doaj.org-article:156b40077d344909b9ce5858c45bcc132021-11-21T21:50:21ZGenetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence1412-033X2085-472210.13057/biodiv/d200631https://doaj.org/article/156b40077d344909b9ce5858c45bcc132019-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3716https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Arif MF, Aristya GR, Kasiamdari RS. 2019. Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence. Biodiversitas 20: 1721-1728. Banyuroto Village, Magelang, Central Java is the center of strawberry (Fragaria spp.) development and cultivation program. The mild climate makes the location very suitable for developing sub-tropic horticulture such as strawberry. Various kinds of cultivars have been developed as part of the agrotourism program. However, genetic variation research of those cultivars is still rarely done. Therefore, the molecular study of strawberry was conducted to determine the genetic variation using CAPS markers. DNA of Five strawberry cultivars from Banyuroto and seven strawberry cultivars from Indonesian Citrus and Subtropical Fruits Research Institute, Malang were isolated using the CTAB method. DNA amplification was performed by PCR using four pairs of primers named APx, OLP, F3H2, and CTI2. The amplification results were cut with three kinds of restriction endonuclease enzymes named MboI, MluI, and TaqI. Restriction product was used to analyze the genetic variation of twelve cultivars and to construct the dendrogram using MVSP software with the UPGMA algorithm. The result showed that the percentage of polymorphic was 45% from 9 polymorphic bands. Dendrogram result showed that there were four clusters. Cluster A consists of Stroberi Hitam, Cluster B consists of Californica Cultivars, cluster C consists of Osso Grande and Osso Purbalingga cultivars, and cluster D consists of Rosalinda, Deeprose, Dorit, Earlibrite, Tristar, Festival, Brastagi, and Aerut. This research concluded that CAPS markers can be used as a method for study genetic diversity of strawberries.MUHAMMAD FAUZI ARIFGANIES RIZA ARISTYARINA SRI KASIAMDARIMBI & UNS Soloarticlecaps, fragaria, genetic variation, molecular markers, strawberry, restriction enzymeBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	caps, fragaria, genetic variation, molecular markers, strawberry, restriction enzyme Biology (General) QH301-705.5
spellingShingle	caps, fragaria, genetic variation, molecular markers, strawberry, restriction enzyme Biology (General) QH301-705.5 MUHAMMAD FAUZI ARIF GANIES RIZA ARISTYA RINA SRI KASIAMDARI Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
description	Abstract. Arif MF, Aristya GR, Kasiamdari RS. 2019. Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence. Biodiversitas 20: 1721-1728. Banyuroto Village, Magelang, Central Java is the center of strawberry (Fragaria spp.) development and cultivation program. The mild climate makes the location very suitable for developing sub-tropic horticulture such as strawberry. Various kinds of cultivars have been developed as part of the agrotourism program. However, genetic variation research of those cultivars is still rarely done. Therefore, the molecular study of strawberry was conducted to determine the genetic variation using CAPS markers. DNA of Five strawberry cultivars from Banyuroto and seven strawberry cultivars from Indonesian Citrus and Subtropical Fruits Research Institute, Malang were isolated using the CTAB method. DNA amplification was performed by PCR using four pairs of primers named APx, OLP, F3H2, and CTI2. The amplification results were cut with three kinds of restriction endonuclease enzymes named MboI, MluI, and TaqI. Restriction product was used to analyze the genetic variation of twelve cultivars and to construct the dendrogram using MVSP software with the UPGMA algorithm. The result showed that the percentage of polymorphic was 45% from 9 polymorphic bands. Dendrogram result showed that there were four clusters. Cluster A consists of Stroberi Hitam, Cluster B consists of Californica Cultivars, cluster C consists of Osso Grande and Osso Purbalingga cultivars, and cluster D consists of Rosalinda, Deeprose, Dorit, Earlibrite, Tristar, Festival, Brastagi, and Aerut. This research concluded that CAPS markers can be used as a method for study genetic diversity of strawberries.
format	article
author	MUHAMMAD FAUZI ARIF GANIES RIZA ARISTYA RINA SRI KASIAMDARI
author_facet	MUHAMMAD FAUZI ARIF GANIES RIZA ARISTYA RINA SRI KASIAMDARI
author_sort	MUHAMMAD FAUZI ARIF
title	Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
title_short	Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
title_full	Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
title_fullStr	Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
title_full_unstemmed	Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence
title_sort	genetic diversity of strawberry cultivars in banyuroto, magelang, indonesia based on cleaved amplified polymorphic sequence
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/156b40077d344909b9ce5858c45bcc13
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_version_	1718418626314240000

Genetic diversity of strawberry cultivars in Banyuroto, Magelang, Indonesia based on Cleaved Amplified Polymorphic Sequence

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