Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.

In many organisms, homologous pairing and synapsis depend on the meiotic recombination machinery that repairs double-strand DNA breaks (DSBs) produced at the onset of meiosis. The culmination of recombination via crossover gives rise to chiasmata, which locate distally in many plant species such as...

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Autores principales:	Nohelia T Valenzuela, Esther Perera, Tomás Naranjo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/156bf2ea6ebc440ebad84195a749109f
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spelling	oai:doaj.org-article:156bf2ea6ebc440ebad84195a749109f2021-11-18T07:20:08ZDynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.1932-620310.1371/journal.pone.0036385https://doaj.org/article/156bf2ea6ebc440ebad84195a749109f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558456/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In many organisms, homologous pairing and synapsis depend on the meiotic recombination machinery that repairs double-strand DNA breaks (DSBs) produced at the onset of meiosis. The culmination of recombination via crossover gives rise to chiasmata, which locate distally in many plant species such as rye, Secale cereale. Although, synapsis initiates close to the chromosome ends, a direct effect of regions with high crossover frequency on partner identification and synapsis initiation has not been demonstrated. Here, we analyze the dynamics of distal and proximal regions of a rye chromosome introgressed into wheat to define their role on meiotic homology search and synapsis. We have used lines with a pair of two-armed chromosome 1R of rye, or a pair of telocentrics of its long arm (1RL), which were homozygous for the standard 1RL structure, homozygous for an inversion of 1RL that changes chiasma location from distal to proximal, or heterozygous for the inversion. Physical mapping of recombination produced in the ditelocentric heterozygote (1RL/1RL(inv)) showed that 70% of crossovers in the arm were confined to a terminal segment representing 10% of the 1RL length. The dynamics of the arms 1RL and 1RL(inv) during zygotene demonstrates that crossover-rich regions are more active in recognizing the homologous partner and developing synapsis than crossover-poor regions. When the crossover-rich regions are positioned in the vicinity of chromosome ends, their association is facilitated by telomere clustering; when they are positioned centrally in one of the two-armed chromosomes and distally in the homolog, their association is probably derived from chromosome elongation. On the other hand, chromosome movements that disassemble the bouquet may facilitate chromosome pairing correction by dissolution of improper chromosome associations. Taken together, these data support that repair of DSBs via crossover is essential in both the search of the homologous partner and consolidation of homologous synapsis.Nohelia T ValenzuelaEsther PereraTomás NaranjoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e36385 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nohelia T Valenzuela Esther Perera Tomás Naranjo Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
description	In many organisms, homologous pairing and synapsis depend on the meiotic recombination machinery that repairs double-strand DNA breaks (DSBs) produced at the onset of meiosis. The culmination of recombination via crossover gives rise to chiasmata, which locate distally in many plant species such as rye, Secale cereale. Although, synapsis initiates close to the chromosome ends, a direct effect of regions with high crossover frequency on partner identification and synapsis initiation has not been demonstrated. Here, we analyze the dynamics of distal and proximal regions of a rye chromosome introgressed into wheat to define their role on meiotic homology search and synapsis. We have used lines with a pair of two-armed chromosome 1R of rye, or a pair of telocentrics of its long arm (1RL), which were homozygous for the standard 1RL structure, homozygous for an inversion of 1RL that changes chiasma location from distal to proximal, or heterozygous for the inversion. Physical mapping of recombination produced in the ditelocentric heterozygote (1RL/1RL(inv)) showed that 70% of crossovers in the arm were confined to a terminal segment representing 10% of the 1RL length. The dynamics of the arms 1RL and 1RL(inv) during zygotene demonstrates that crossover-rich regions are more active in recognizing the homologous partner and developing synapsis than crossover-poor regions. When the crossover-rich regions are positioned in the vicinity of chromosome ends, their association is facilitated by telomere clustering; when they are positioned centrally in one of the two-armed chromosomes and distally in the homolog, their association is probably derived from chromosome elongation. On the other hand, chromosome movements that disassemble the bouquet may facilitate chromosome pairing correction by dissolution of improper chromosome associations. Taken together, these data support that repair of DSBs via crossover is essential in both the search of the homologous partner and consolidation of homologous synapsis.
format	article
author	Nohelia T Valenzuela Esther Perera Tomás Naranjo
author_facet	Nohelia T Valenzuela Esther Perera Tomás Naranjo
author_sort	Nohelia T Valenzuela
title	Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
title_short	Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
title_full	Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
title_fullStr	Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
title_full_unstemmed	Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.
title_sort	dynamics of rye chromosome 1r regions with high or low crossover frequency in homology search and synapsis development.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/156bf2ea6ebc440ebad84195a749109f
work_keys_str_mv	AT noheliatvalenzuela dynamicsofryechromosome1rregionswithhighorlowcrossoverfrequencyinhomologysearchandsynapsisdevelopment AT estherperera dynamicsofryechromosome1rregionswithhighorlowcrossoverfrequencyinhomologysearchandsynapsisdevelopment AT tomasnaranjo dynamicsofryechromosome1rregionswithhighorlowcrossoverfrequencyinhomologysearchandsynapsisdevelopment
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Dynamics of rye chromosome 1R regions with high or low crossover frequency in homology search and synapsis development.

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