Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids

Abstract Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein cha...

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Autores principales:	Lisa M. Leung, William E. Fondrie, Yohei Doi, J. Kristie Johnson, Dudley K. Strickland, Robert K. Ernst, David R. Goodlett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/156f61f087a64257a20eeb277fb83d6c
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spelling	oai:doaj.org-article:156f61f087a64257a20eeb277fb83d6c2021-12-02T12:32:44ZIdentification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids10.1038/s41598-017-04793-42045-2322https://doaj.org/article/156f61f087a64257a20eeb277fb83d6c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04793-4https://doaj.org/toc/2045-2322Abstract Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories. Here, we present a novel and complementary approach that utilizes essential microbial glycolipids as chemical fingerprints for identification of individual bacterial species. Gram-positive and negative bacterial glycolipids were extracted using a single optimized protocol. Extracts of the clinically significant ESKAPE pathogens: E nterococcus faecium, S taphylococcus aureus, K lebsiella pneumoniae, A cinetobacter baumannii, P seudomonas aeruginosa, and E nterobacter spp. were analyzed by MALDI-TOF-MS in negative ion mode to obtain glycolipid mass spectra. A library of glycolipid mass spectra from 50 microbial entries was developed that allowed bacterial speciation of the ESKAPE pathogens, as well as identification of pathogens directly from blood bottles without culture on solid medium and determination of antimicrobial peptide resistance. These results demonstrate that bacterial glycolipid mass spectra represent chemical barcodes that identify pathogens, potentially providing a useful alternative to existing diagnostics.Lisa M. LeungWilliam E. FondrieYohei DoiJ. Kristie JohnsonDudley K. StricklandRobert K. ErnstDavid R. GoodlettNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa M. Leung William E. Fondrie Yohei Doi J. Kristie Johnson Dudley K. Strickland Robert K. Ernst David R. Goodlett Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
description	Abstract Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories. Here, we present a novel and complementary approach that utilizes essential microbial glycolipids as chemical fingerprints for identification of individual bacterial species. Gram-positive and negative bacterial glycolipids were extracted using a single optimized protocol. Extracts of the clinically significant ESKAPE pathogens: E nterococcus faecium, S taphylococcus aureus, K lebsiella pneumoniae, A cinetobacter baumannii, P seudomonas aeruginosa, and E nterobacter spp. were analyzed by MALDI-TOF-MS in negative ion mode to obtain glycolipid mass spectra. A library of glycolipid mass spectra from 50 microbial entries was developed that allowed bacterial speciation of the ESKAPE pathogens, as well as identification of pathogens directly from blood bottles without culture on solid medium and determination of antimicrobial peptide resistance. These results demonstrate that bacterial glycolipid mass spectra represent chemical barcodes that identify pathogens, potentially providing a useful alternative to existing diagnostics.
format	article
author	Lisa M. Leung William E. Fondrie Yohei Doi J. Kristie Johnson Dudley K. Strickland Robert K. Ernst David R. Goodlett
author_facet	Lisa M. Leung William E. Fondrie Yohei Doi J. Kristie Johnson Dudley K. Strickland Robert K. Ernst David R. Goodlett
author_sort	Lisa M. Leung
title	Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
title_short	Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
title_full	Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
title_fullStr	Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
title_full_unstemmed	Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids
title_sort	identification of the eskape pathogens by mass spectrometric analysis of microbial membrane glycolipids
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/156f61f087a64257a20eeb277fb83d6c
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Identification of the ESKAPE pathogens by mass spectrometric analysis of microbial membrane glycolipids

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