A novel and sensitive real-time PCR system for universal detection of poxviruses
Abstract Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (...
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Nature Portfolio
2021
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oai:doaj.org-article:156fc1a030e3419f8a4736e6d3c6c6d52021-12-02T13:51:15ZA novel and sensitive real-time PCR system for universal detection of poxviruses10.1038/s41598-021-81376-42045-2322https://doaj.org/article/156fc1a030e3419f8a4736e6d3c6c6d52021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81376-4https://doaj.org/toc/2045-2322Abstract Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects.Léa LucianiLucia InchausteOlivier FerrarisRémi CharrelAntoine NougairèdeGéraldine PiorkowskiChristophe PeyrefitteStéphane BertagnoliXavier de LamballerieStéphane PrietNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Léa Luciani Lucia Inchauste Olivier Ferraris Rémi Charrel Antoine Nougairède Géraldine Piorkowski Christophe Peyrefitte Stéphane Bertagnoli Xavier de Lamballerie Stéphane Priet A novel and sensitive real-time PCR system for universal detection of poxviruses |
| description |
Abstract Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects. |
| format |
article |
| author |
Léa Luciani Lucia Inchauste Olivier Ferraris Rémi Charrel Antoine Nougairède Géraldine Piorkowski Christophe Peyrefitte Stéphane Bertagnoli Xavier de Lamballerie Stéphane Priet |
| author_facet |
Léa Luciani Lucia Inchauste Olivier Ferraris Rémi Charrel Antoine Nougairède Géraldine Piorkowski Christophe Peyrefitte Stéphane Bertagnoli Xavier de Lamballerie Stéphane Priet |
| author_sort |
Léa Luciani |
| title |
A novel and sensitive real-time PCR system for universal detection of poxviruses |
| title_short |
A novel and sensitive real-time PCR system for universal detection of poxviruses |
| title_full |
A novel and sensitive real-time PCR system for universal detection of poxviruses |
| title_fullStr |
A novel and sensitive real-time PCR system for universal detection of poxviruses |
| title_full_unstemmed |
A novel and sensitive real-time PCR system for universal detection of poxviruses |
| title_sort |
novel and sensitive real-time pcr system for universal detection of poxviruses |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/156fc1a030e3419f8a4736e6d3c6c6d5 |
| work_keys_str_mv |
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