Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge

Abstract Negative Energy Balance (NEB) is considered to increase susceptibility to mastitis. The objective of this study was to improve our understanding of the underlying mechanisms by comparing transcriptomic profiles following NEB and a concomitant mammary inflammation. Accordingly, we performed...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juliette Bouvier-Muller, Charlotte Allain, Guillaume Tabouret, Francis Enjalbert, David Portes, Céline Noirot, Rachel Rupp, Gilles Foucras
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15707cc28fb14e7eb7b40067254aaa1d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:15707cc28fb14e7eb7b40067254aaa1d
record_format	dspace
spelling	oai:doaj.org-article:15707cc28fb14e7eb7b40067254aaa1d2021-12-02T11:40:50ZWhole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge10.1038/s41598-017-02391-y2045-2322https://doaj.org/article/15707cc28fb14e7eb7b40067254aaa1d2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02391-yhttps://doaj.org/toc/2045-2322Abstract Negative Energy Balance (NEB) is considered to increase susceptibility to mastitis. The objective of this study was to improve our understanding of the underlying mechanisms by comparing transcriptomic profiles following NEB and a concomitant mammary inflammation. Accordingly, we performed RNA-seq analysis of blood cells in energy-restricted ewes and control-diet ewes at four different time points before and after intra mammary challenge with phlogogenic ligands. Blood leucocytes responded to NEB by shutting down lipid-generating processes, including cholesterol and fatty acid synthesis, probably under transcriptional control of SREBF 1. Furthermore, fatty acid oxidation was activated and glucose oxidation and transport inhibited in response to energy restriction. Among the differentially expressed genes (DEGs) in response to energy restriction, 64 genes were also differential in response to the inflammatory challenge. Opposite response included the activation of cholesterol and fatty acid synthesis during the inflammatory challenge. Moreover, activation of glucose oxidation and transport coupled with the increase of plasma glucose concentration in response to the inflammatory stimuli suggested a preferential utilization of glucose as the energy source during this stress. Leucocyte metabolism therefore undergoes strong metabolic changes during an inflammatory challenge, which could be in competition with those induced by energy restriction.Juliette Bouvier-MullerCharlotte AllainGuillaume TabouretFrancis EnjalbertDavid PortesCéline NoirotRachel RuppGilles FoucrasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juliette Bouvier-Muller Charlotte Allain Guillaume Tabouret Francis Enjalbert David Portes Céline Noirot Rachel Rupp Gilles Foucras Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
description	Abstract Negative Energy Balance (NEB) is considered to increase susceptibility to mastitis. The objective of this study was to improve our understanding of the underlying mechanisms by comparing transcriptomic profiles following NEB and a concomitant mammary inflammation. Accordingly, we performed RNA-seq analysis of blood cells in energy-restricted ewes and control-diet ewes at four different time points before and after intra mammary challenge with phlogogenic ligands. Blood leucocytes responded to NEB by shutting down lipid-generating processes, including cholesterol and fatty acid synthesis, probably under transcriptional control of SREBF 1. Furthermore, fatty acid oxidation was activated and glucose oxidation and transport inhibited in response to energy restriction. Among the differentially expressed genes (DEGs) in response to energy restriction, 64 genes were also differential in response to the inflammatory challenge. Opposite response included the activation of cholesterol and fatty acid synthesis during the inflammatory challenge. Moreover, activation of glucose oxidation and transport coupled with the increase of plasma glucose concentration in response to the inflammatory stimuli suggested a preferential utilization of glucose as the energy source during this stress. Leucocyte metabolism therefore undergoes strong metabolic changes during an inflammatory challenge, which could be in competition with those induced by energy restriction.
format	article
author	Juliette Bouvier-Muller Charlotte Allain Guillaume Tabouret Francis Enjalbert David Portes Céline Noirot Rachel Rupp Gilles Foucras
author_facet	Juliette Bouvier-Muller Charlotte Allain Guillaume Tabouret Francis Enjalbert David Portes Céline Noirot Rachel Rupp Gilles Foucras
author_sort	Juliette Bouvier-Muller
title	Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
title_short	Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
title_full	Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
title_fullStr	Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
title_full_unstemmed	Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
title_sort	whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/15707cc28fb14e7eb7b40067254aaa1d
work_keys_str_mv	AT juliettebouviermuller wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT charlotteallain wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT guillaumetabouret wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT francisenjalbert wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT davidportes wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT celinenoirot wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT rachelrupp wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge AT gillesfoucras wholebloodtranscriptomeanalysisrevealspotentialcompetitioninmetabolicpathwaysbetweennegativeenergybalanceandresponsetoinflammatorychallenge
_version_	1718395505303617536

Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge

Ejemplares similares