Changes in telomere length and senescence markers during human ovarian tissue cryopreservation

Abstract Ovarian tissue cryopreservation is considered as a useful option to preserve fertility for cancer patients. This study purposed to evaluate the change of telomere length and senescence markers during human ovarian tissue cryopreservation. Ovarian tissues were obtained from women who underwe...

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Autores principales:	Boram Kim, Ki-Jin Ryu, Sanghoon Lee, Tak Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1571128309f3448f976fdf9d4b45bb0f
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spelling	oai:doaj.org-article:1571128309f3448f976fdf9d4b45bb0f2021-12-02T13:27:32ZChanges in telomere length and senescence markers during human ovarian tissue cryopreservation10.1038/s41598-021-81973-32045-2322https://doaj.org/article/1571128309f3448f976fdf9d4b45bb0f2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81973-3https://doaj.org/toc/2045-2322Abstract Ovarian tissue cryopreservation is considered as a useful option to preserve fertility for cancer patients. This study purposed to evaluate the change of telomere length and senescence markers during human ovarian tissue cryopreservation. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital with prior consent and IRB approval. DNA was extracted from the ovarian tissues using a DNeasy tissue kit and telomere lengths in the DNA samples were determined by real time PCR before and after cryopreservation. All tissues were stained with hematoxylin–eosin and subjected to immunohistochemistry and TUNEL assays. Other senescence markers, including p53, p16, p21, and phospho-pRb proteins, were evaluated using western blot analysis. Ovarian tissues were collected from ten patients and prepared for slow freezing with the same size of diameter 4 mm and 1 mm thickness. Mean age of patients was 26.7 ± 6.2 years (range, 16–34 years), and ovarian tissues were cryopreserved and thawed 4 weeks after cryopreservation. The mean telomere length was significantly decreased after cryopreservation (9.57 ± 1.47 bp vs. 8.34 ± 1.83 bp, p = 0.001). Western blot analysis revealed that p53, p16, and p21 proteins increased and phospho-pRb protein expression decreased after ovarian tissue cryopreservation. Ovarian tissue cryopreservation and transplantation is regarded as one of promising options for fertility preservation. However, clinicians and researchers should be aware of possible irreversible DNA changes such as shortening of telomere length and alterations of other senescence markers in human ovarian tissues.Boram KimKi-Jin RyuSanghoon LeeTak KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Boram Kim Ki-Jin Ryu Sanghoon Lee Tak Kim Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
description	Abstract Ovarian tissue cryopreservation is considered as a useful option to preserve fertility for cancer patients. This study purposed to evaluate the change of telomere length and senescence markers during human ovarian tissue cryopreservation. Ovarian tissues were obtained from women who underwent benign ovarian surgery in the gynecology research unit of a university hospital with prior consent and IRB approval. DNA was extracted from the ovarian tissues using a DNeasy tissue kit and telomere lengths in the DNA samples were determined by real time PCR before and after cryopreservation. All tissues were stained with hematoxylin–eosin and subjected to immunohistochemistry and TUNEL assays. Other senescence markers, including p53, p16, p21, and phospho-pRb proteins, were evaluated using western blot analysis. Ovarian tissues were collected from ten patients and prepared for slow freezing with the same size of diameter 4 mm and 1 mm thickness. Mean age of patients was 26.7 ± 6.2 years (range, 16–34 years), and ovarian tissues were cryopreserved and thawed 4 weeks after cryopreservation. The mean telomere length was significantly decreased after cryopreservation (9.57 ± 1.47 bp vs. 8.34 ± 1.83 bp, p = 0.001). Western blot analysis revealed that p53, p16, and p21 proteins increased and phospho-pRb protein expression decreased after ovarian tissue cryopreservation. Ovarian tissue cryopreservation and transplantation is regarded as one of promising options for fertility preservation. However, clinicians and researchers should be aware of possible irreversible DNA changes such as shortening of telomere length and alterations of other senescence markers in human ovarian tissues.
format	article
author	Boram Kim Ki-Jin Ryu Sanghoon Lee Tak Kim
author_facet	Boram Kim Ki-Jin Ryu Sanghoon Lee Tak Kim
author_sort	Boram Kim
title	Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
title_short	Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
title_full	Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
title_fullStr	Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
title_full_unstemmed	Changes in telomere length and senescence markers during human ovarian tissue cryopreservation
title_sort	changes in telomere length and senescence markers during human ovarian tissue cryopreservation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1571128309f3448f976fdf9d4b45bb0f
work_keys_str_mv	AT boramkim changesintelomerelengthandsenescencemarkersduringhumanovariantissuecryopreservation AT kijinryu changesintelomerelengthandsenescencemarkersduringhumanovariantissuecryopreservation AT sanghoonlee changesintelomerelengthandsenescencemarkersduringhumanovariantissuecryopreservation AT takkim changesintelomerelengthandsenescencemarkersduringhumanovariantissuecryopreservation
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Changes in telomere length and senescence markers during human ovarian tissue cryopreservation

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