Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion

Abstract Hundreds of thousands of human implant procedures require surgical revision each year due to infection. Infections are difficult to treat with conventional antibiotics due to the formation of biofilm on the implant surface. We have developed a noninvasive method to eliminate biofilm on meta...

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Autores principales:	Qi Wang, Jonathan Vachon, Bibin Prasad, Christine A. Pybus, Norman Lapin, Rajiv Chopra, David E. Greenberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/15746696d2344d83824c8b97671d6bff
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spelling	oai:doaj.org-article:15746696d2344d83824c8b97671d6bff2021-12-02T15:07:47ZAlternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion10.1038/s41522-021-00239-y2055-5008https://doaj.org/article/15746696d2344d83824c8b97671d6bff2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00239-yhttps://doaj.org/toc/2055-5008Abstract Hundreds of thousands of human implant procedures require surgical revision each year due to infection. Infections are difficult to treat with conventional antibiotics due to the formation of biofilm on the implant surface. We have developed a noninvasive method to eliminate biofilm on metal implants using heat generated by intermittent alternating magnetic fields (iAMF). Here, we demonstrate that heat and antibiotics are synergistic in biofilm elimination. For Pseudomonas aeruginosa biofilm, bacterial burden was reduced >3 log with iAMF and ciprofloxacin after 24 h compared with either treatment alone (p < 0.0001). This effect was not limited by pathogen or antibiotic as similar biofilm reductions were seen with iAMF and either linezolid or ceftriaxone in Staphylococcus aureus. iAMF and antibiotic efficacy was seen across various iAMF settings, including different iAMF target temperatures, dose durations, and dosing intervals. Initial mechanistic studies revealed membrane disruption as one factor important for AMF enhanced antibacterial activity in the biofilm setting. This study demonstrates the potential of utilizing a noninvasive approach to reduce biofilm off of metallic implants.Qi WangJonathan VachonBibin PrasadChristine A. PybusNorman LapinRajiv ChopraDavid E. GreenbergNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Qi Wang Jonathan Vachon Bibin Prasad Christine A. Pybus Norman Lapin Rajiv Chopra David E. Greenberg Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
description	Abstract Hundreds of thousands of human implant procedures require surgical revision each year due to infection. Infections are difficult to treat with conventional antibiotics due to the formation of biofilm on the implant surface. We have developed a noninvasive method to eliminate biofilm on metal implants using heat generated by intermittent alternating magnetic fields (iAMF). Here, we demonstrate that heat and antibiotics are synergistic in biofilm elimination. For Pseudomonas aeruginosa biofilm, bacterial burden was reduced >3 log with iAMF and ciprofloxacin after 24 h compared with either treatment alone (p < 0.0001). This effect was not limited by pathogen or antibiotic as similar biofilm reductions were seen with iAMF and either linezolid or ceftriaxone in Staphylococcus aureus. iAMF and antibiotic efficacy was seen across various iAMF settings, including different iAMF target temperatures, dose durations, and dosing intervals. Initial mechanistic studies revealed membrane disruption as one factor important for AMF enhanced antibacterial activity in the biofilm setting. This study demonstrates the potential of utilizing a noninvasive approach to reduce biofilm off of metallic implants.
format	article
author	Qi Wang Jonathan Vachon Bibin Prasad Christine A. Pybus Norman Lapin Rajiv Chopra David E. Greenberg
author_facet	Qi Wang Jonathan Vachon Bibin Prasad Christine A. Pybus Norman Lapin Rajiv Chopra David E. Greenberg
author_sort	Qi Wang
title	Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
title_short	Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
title_full	Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
title_fullStr	Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
title_full_unstemmed	Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
title_sort	alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15746696d2344d83824c8b97671d6bff
work_keys_str_mv	AT qiwang alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT jonathanvachon alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT bibinprasad alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT christineapybus alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT normanlapin alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT rajivchopra alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion AT davidegreenberg alternatingmagneticfieldsandantibioticseradicatebiofilmonmetalinasynergisticfashion
_version_	1718388367809314816

Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion

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