Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a common pathogen that causes various serious diseases, including chronic infections. Discovering new antibacterial agents is an important aspect of the pharmaceutical field because of the lack of effective antibacterial drug...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/157df331543e4d0fba439efa1cb4055e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a common pathogen that causes various serious diseases, including chronic infections. Discovering new antibacterial agents is an important aspect of the pharmaceutical field because of the lack of effective antibacterial drugs. In our research, we found that one anti-<i>S. aureus</i> substance is actinomycin D, originating from <i>Streptomyces parvulus</i> (<i>S. parvulus</i>); then, we further focused on the anti-<i>S. aureus</i> ability and the omics profile of <i>S. aureus</i> in response to actinomycin D. The results revealed that actinomycin D had a significant inhibitory activity on <i>S. aureus</i> with a minimum inhibitory concentration (MIC) of 2 μg/mL and a minimum bactericidal concentration (MBC) of 64 μg/mL. Bacterial reactive oxygen species (ROS) increased 3.5-fold upon treatment with actinomycin D, as was measured with the oxidation-sensitive fluorescent probe DCFH-DA, and H<sub>2</sub>O<sub>2</sub> increased 3.5 times with treatment by actinomycin D. Proteomics and metabolomics, respectively, identified differentially expressed proteins in control and treatment groups, and the co-mapped correlation network of proteomics and metabolomics annotated five major pathways that were potentially related to disrupting the energy metabolism and oxidative stress of <i>S. aureus</i>. All findings contributed to providing new insight into the mechanisms of the anti-<i>S. aureus</i> effects of actinomycin D originating from <i>S. parvulus.</i> |
|---|