Multi-Omics Analysis Reveals Anti-Staphylococcus aureus Activity of Actinomycin D Originating from Streptomyces parvulus

Staphylococcus aureus (S. aureus) is a common pathogen that causes various serious diseases, including chronic infections. Discovering new antibacterial agents is an important aspect of the pharmaceutical field because of the lack of effective antibacterial drug...

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Autores principales:	Yuqi Lin, Li Huang, Xiaoyong Zhang, Jiajia Yang, Xiaodan Chen, Fengming Li, Jun Liu, Riming Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Staphylococcus aureus</i> <i>Streptomyces parvulus</i> actinomycin D proteomic and metabolomic profiles Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/157df331543e4d0fba439efa1cb4055e
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spelling	oai:doaj.org-article:157df331543e4d0fba439efa1cb4055e2021-11-25T17:54:33ZMulti-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>10.3390/ijms2222122311422-00671661-6596https://doaj.org/article/157df331543e4d0fba439efa1cb4055e2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12231https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a common pathogen that causes various serious diseases, including chronic infections. Discovering new antibacterial agents is an important aspect of the pharmaceutical field because of the lack of effective antibacterial drugs. In our research, we found that one anti-<i>S. aureus</i> substance is actinomycin D, originating from <i>Streptomyces parvulus</i> (<i>S. parvulus</i>); then, we further focused on the anti-<i>S. aureus</i> ability and the omics profile of <i>S. aureus</i> in response to actinomycin D. The results revealed that actinomycin D had a significant inhibitory activity on <i>S. aureus</i> with a minimum inhibitory concentration (MIC) of 2 μg/mL and a minimum bactericidal concentration (MBC) of 64 μg/mL. Bacterial reactive oxygen species (ROS) increased 3.5-fold upon treatment with actinomycin D, as was measured with the oxidation-sensitive fluorescent probe DCFH-DA, and H<sub>2</sub>O<sub>2</sub> increased 3.5 times with treatment by actinomycin D. Proteomics and metabolomics, respectively, identified differentially expressed proteins in control and treatment groups, and the co-mapped correlation network of proteomics and metabolomics annotated five major pathways that were potentially related to disrupting the energy metabolism and oxidative stress of <i>S. aureus</i>. All findings contributed to providing new insight into the mechanisms of the anti-<i>S. aureus</i> effects of actinomycin D originating from <i>S. parvulus.</i>Yuqi LinLi HuangXiaoyong ZhangJiajia YangXiaodan ChenFengming LiJun LiuRiming HuangMDPI AGarticle<i>Staphylococcus aureus</i><i>Streptomyces parvulus</i>actinomycin Dproteomic and metabolomic profilesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12231, p 12231 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Staphylococcus aureus</i> <i>Streptomyces parvulus</i> actinomycin D proteomic and metabolomic profiles Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Staphylococcus aureus</i> <i>Streptomyces parvulus</i> actinomycin D proteomic and metabolomic profiles Biology (General) QH301-705.5 Chemistry QD1-999 Yuqi Lin Li Huang Xiaoyong Zhang Jiajia Yang Xiaodan Chen Fengming Li Jun Liu Riming Huang Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
description	<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a common pathogen that causes various serious diseases, including chronic infections. Discovering new antibacterial agents is an important aspect of the pharmaceutical field because of the lack of effective antibacterial drugs. In our research, we found that one anti-<i>S. aureus</i> substance is actinomycin D, originating from <i>Streptomyces parvulus</i> (<i>S. parvulus</i>); then, we further focused on the anti-<i>S. aureus</i> ability and the omics profile of <i>S. aureus</i> in response to actinomycin D. The results revealed that actinomycin D had a significant inhibitory activity on <i>S. aureus</i> with a minimum inhibitory concentration (MIC) of 2 μg/mL and a minimum bactericidal concentration (MBC) of 64 μg/mL. Bacterial reactive oxygen species (ROS) increased 3.5-fold upon treatment with actinomycin D, as was measured with the oxidation-sensitive fluorescent probe DCFH-DA, and H<sub>2</sub>O<sub>2</sub> increased 3.5 times with treatment by actinomycin D. Proteomics and metabolomics, respectively, identified differentially expressed proteins in control and treatment groups, and the co-mapped correlation network of proteomics and metabolomics annotated five major pathways that were potentially related to disrupting the energy metabolism and oxidative stress of <i>S. aureus</i>. All findings contributed to providing new insight into the mechanisms of the anti-<i>S. aureus</i> effects of actinomycin D originating from <i>S. parvulus.</i>
format	article
author	Yuqi Lin Li Huang Xiaoyong Zhang Jiajia Yang Xiaodan Chen Fengming Li Jun Liu Riming Huang
author_facet	Yuqi Lin Li Huang Xiaoyong Zhang Jiajia Yang Xiaodan Chen Fengming Li Jun Liu Riming Huang
author_sort	Yuqi Lin
title	Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
title_short	Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
title_full	Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
title_fullStr	Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
title_full_unstemmed	Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>
title_sort	multi-omics analysis reveals anti-<i>staphylococcus aureus</i> activity of actinomycin d originating from <i>streptomyces parvulus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/157df331543e4d0fba439efa1cb4055e
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Multi-Omics Analysis Reveals Anti-<i>Staphylococcus aureus</i> Activity of Actinomycin D Originating from <i>Streptomyces parvulus</i>

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