Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human

Staphylococcus aureus represents a serious health hazard on both animals and humans. The main goal of the present work was to compare between S. aureus isolated from animal and human origin. This was achieved through studying the virulence, phenotypic characterization, genotypic testing of mecA gene...

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Autores principales:	Amira Fikry, Abd El-Rady Thabet Ahmed, Ahmed Samir, Heidy Abo El- Yazeed, Khaled F. El-Amry, Heba N. Deif
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	cows human mdr meca gene mrsa s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.159379 https://doaj.org/article/157ed727bb5c44ecb9a4cf8e21bfe3cf
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spelling	oai:doaj.org-article:157ed727bb5c44ecb9a4cf8e21bfe3cf2021-12-02T14:28:01ZBacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Humanhttps://dx.doi.org/10.21608/javs.2021.1593791687-40722090-3308https://doaj.org/article/157ed727bb5c44ecb9a4cf8e21bfe3cf2021-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_159379.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Staphylococcus aureus represents a serious health hazard on both animals and humans. The main goal of the present work was to compare between S. aureus isolated from animal and human origin. This was achieved through studying the virulence, phenotypic characterization, genotypic testing of mecA gene, analysis the antibiotic resistance profile of the recovered S. aureus isolates. A total number of 165 samples were collected from clinical mastitic cows and sheep, pus from abscesses collected from septic wounds of infected animals, respectively. Moreover, blood, pus swabs from abscesses and septic wounds, and sputum samples were collected from diseased humans from Assiut Governorate, Egypt. The results revealed that incidence of S. aureus isolates recovered from the examined animal samples were 8.33%, 100% and 20%, per, while from human cases (blood, pus and sputum) were 16.67%, 53.49% and 75%, independently. Using Staphaurex kits, the prevalence of coagulase positive S. aureus from animal samples reached 8.33%, 100% and 20%, but from the diseased human cases were 16.67%, 53.49% and 75%, respectively. Antimicrobial Sensitivity Testing of the animal isolates were resistant to cefoxitin (15.79%), tetracycline (10.53%), clindamycin and erythromycin (10.53%), while the isolates were sensitive to gentamicin (100%), trimethoprim + sulfamethoxazole and vancomycin (94.74% for each). However, S. aureus human isolates were resistant to cefoxitin (75%) and tetracycline (78.57%) and sensitive to vancomycin (100%), ciprofloxacin (89.29%) and trimethoprim+ sulfamethoxazole (82.14%). Out of 47 identified S. aureus strains, 3 from animal isolates (15.79%) and 21 from human isolates (75%) proved to be methicillin-resistant (MRSA). Furthermore, one animal isolate (5.26%) and 12 human isolates (42.86%) were multi-drug resistant (MDR). S. aureus isolates from animal and humans were subjected to genotypic characterization of mecA gene using PCR. All the animal and human isolates were positive for mecA gene with a percentage of 100%. The results of this study provide that from both animals and human samples; the isolation rate of S. aureus was greater than that of MRSA followed by MDR. Amira FikryAbd El-Rady Thabet AhmedAhmed SamirHeidy Abo El- YazeedKhaled F. El-AmryHeba N. DeifEgyptian Society for Animal Managementarticlecowshumanmdrmeca genemrsas. aureusZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 2, Pp 44-52 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cows human mdr meca gene mrsa s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	cows human mdr meca gene mrsa s. aureus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Amira Fikry Abd El-Rady Thabet Ahmed Ahmed Samir Heidy Abo El- Yazeed Khaled F. El-Amry Heba N. Deif Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
description	Staphylococcus aureus represents a serious health hazard on both animals and humans. The main goal of the present work was to compare between S. aureus isolated from animal and human origin. This was achieved through studying the virulence, phenotypic characterization, genotypic testing of mecA gene, analysis the antibiotic resistance profile of the recovered S. aureus isolates. A total number of 165 samples were collected from clinical mastitic cows and sheep, pus from abscesses collected from septic wounds of infected animals, respectively. Moreover, blood, pus swabs from abscesses and septic wounds, and sputum samples were collected from diseased humans from Assiut Governorate, Egypt. The results revealed that incidence of S. aureus isolates recovered from the examined animal samples were 8.33%, 100% and 20%, per, while from human cases (blood, pus and sputum) were 16.67%, 53.49% and 75%, independently. Using Staphaurex kits, the prevalence of coagulase positive S. aureus from animal samples reached 8.33%, 100% and 20%, but from the diseased human cases were 16.67%, 53.49% and 75%, respectively. Antimicrobial Sensitivity Testing of the animal isolates were resistant to cefoxitin (15.79%), tetracycline (10.53%), clindamycin and erythromycin (10.53%), while the isolates were sensitive to gentamicin (100%), trimethoprim + sulfamethoxazole and vancomycin (94.74% for each). However, S. aureus human isolates were resistant to cefoxitin (75%) and tetracycline (78.57%) and sensitive to vancomycin (100%), ciprofloxacin (89.29%) and trimethoprim+ sulfamethoxazole (82.14%). Out of 47 identified S. aureus strains, 3 from animal isolates (15.79%) and 21 from human isolates (75%) proved to be methicillin-resistant (MRSA). Furthermore, one animal isolate (5.26%) and 12 human isolates (42.86%) were multi-drug resistant (MDR). S. aureus isolates from animal and humans were subjected to genotypic characterization of mecA gene using PCR. All the animal and human isolates were positive for mecA gene with a percentage of 100%. The results of this study provide that from both animals and human samples; the isolation rate of S. aureus was greater than that of MRSA followed by MDR.
format	article
author	Amira Fikry Abd El-Rady Thabet Ahmed Ahmed Samir Heidy Abo El- Yazeed Khaled F. El-Amry Heba N. Deif
author_facet	Amira Fikry Abd El-Rady Thabet Ahmed Ahmed Samir Heidy Abo El- Yazeed Khaled F. El-Amry Heba N. Deif
author_sort	Amira Fikry
title	Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
title_short	Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
title_full	Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
title_fullStr	Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
title_full_unstemmed	Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
title_sort	bacteriological and molecular comparative study between staphylococcus aureus isolated from animals and human
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.159379 https://doaj.org/article/157ed727bb5c44ecb9a4cf8e21bfe3cf
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Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human

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