Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.

<h4>Background</h4>Staphylococcus epidermidis is a common cause of nosocomial infections worldwide. This study analyzed the differences in genetic endowment and clonal lineages with pathogenesis and resistance traits of S. epidermidis isolates collected from community and hospital enviro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xin Du, Yuanjun Zhu, Yan Song, Tianming Li, Tao Luo, Gang Sun, Chongguang Yang, Cuiming Cao, Yuan Lu, Min Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/158effb4403947b5bd7094ca1677bfce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:158effb4403947b5bd7094ca1677bfce
record_format	dspace
spelling	oai:doaj.org-article:158effb4403947b5bd7094ca1677bfce2021-11-18T07:46:01ZMolecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.1932-620310.1371/journal.pone.0062742https://doaj.org/article/158effb4403947b5bd7094ca1677bfce2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23675424/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Staphylococcus epidermidis is a common cause of nosocomial infections worldwide. This study analyzed the differences in genetic endowment and clonal lineages with pathogenesis and resistance traits of S. epidermidis isolates collected from community and hospital environments (patients and healthcare staff) of the same ecological niche, time period, and geographical location in China.<h4>Methodology/principal findings</h4>Molecular epidemiology and population analysis showed that nasal colonization rates of S. epidermidis in the community of Shanghai area of China and in healthcare personnel were 44.8% (methicillin-resistant S. epidermidis, MRSE: 17.2%) and 61.3% (MRSE: 30.0%), respectively. 86.7% of clinical isolates were MRSE. Among the strains studied, 44 sequence types (STs) were identified with 91.7% belonging to clonal complex 2 (CC2). Only 40.8% isolates from patients were also found in healthy individuals. MRSE-ST2-SCCmecIII was the predominant clone in clinical isolates, almost resistant to all antibiotics tested. Biofilm-related genes IS256 and icaA were detected in majority of the predominant clinical MRSE-ST2 clone with a 40.5% biofilm-positive rate. No ST2 isolate was found in community setting. We found a high prevalence of arginine catabolic mobile element (ACME) (74.1%). The prevalence of ACME-arc and ACME-opp3 clusters was 71.6% and 32.4%, respectively. Methicillin-sensitive S. epidermidis (MSSE) isolates harbored more ACME (83.3%) than MRSE isolates (67.7%), and there was no association between ACME and SCCmec types. An association was found between low-level ACME presence and invasive infections.<h4>Conclusions/significance</h4>We observed a high level of diversity within S. epidermidis in this study, with CC2 as the dominant clonal complex in both community and hospital settings. Only 40.8% of the isolates from patients were also found in healthy individuals. Contrary to that biofilm formation and multiple antibiotic resistance were associated closely with pathogenicity of S. epidermidis, ACME was more likely to be an indicator for colonization rather than a virulence factor.Xin DuYuanjun ZhuYan SongTianming LiTao LuoGang SunChongguang YangCuiming CaoYuan LuMin LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62742 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Du Yuanjun Zhu Yan Song Tianming Li Tao Luo Gang Sun Chongguang Yang Cuiming Cao Yuan Lu Min Li Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
description	<h4>Background</h4>Staphylococcus epidermidis is a common cause of nosocomial infections worldwide. This study analyzed the differences in genetic endowment and clonal lineages with pathogenesis and resistance traits of S. epidermidis isolates collected from community and hospital environments (patients and healthcare staff) of the same ecological niche, time period, and geographical location in China.<h4>Methodology/principal findings</h4>Molecular epidemiology and population analysis showed that nasal colonization rates of S. epidermidis in the community of Shanghai area of China and in healthcare personnel were 44.8% (methicillin-resistant S. epidermidis, MRSE: 17.2%) and 61.3% (MRSE: 30.0%), respectively. 86.7% of clinical isolates were MRSE. Among the strains studied, 44 sequence types (STs) were identified with 91.7% belonging to clonal complex 2 (CC2). Only 40.8% isolates from patients were also found in healthy individuals. MRSE-ST2-SCCmecIII was the predominant clone in clinical isolates, almost resistant to all antibiotics tested. Biofilm-related genes IS256 and icaA were detected in majority of the predominant clinical MRSE-ST2 clone with a 40.5% biofilm-positive rate. No ST2 isolate was found in community setting. We found a high prevalence of arginine catabolic mobile element (ACME) (74.1%). The prevalence of ACME-arc and ACME-opp3 clusters was 71.6% and 32.4%, respectively. Methicillin-sensitive S. epidermidis (MSSE) isolates harbored more ACME (83.3%) than MRSE isolates (67.7%), and there was no association between ACME and SCCmec types. An association was found between low-level ACME presence and invasive infections.<h4>Conclusions/significance</h4>We observed a high level of diversity within S. epidermidis in this study, with CC2 as the dominant clonal complex in both community and hospital settings. Only 40.8% of the isolates from patients were also found in healthy individuals. Contrary to that biofilm formation and multiple antibiotic resistance were associated closely with pathogenicity of S. epidermidis, ACME was more likely to be an indicator for colonization rather than a virulence factor.
format	article
author	Xin Du Yuanjun Zhu Yan Song Tianming Li Tao Luo Gang Sun Chongguang Yang Cuiming Cao Yuan Lu Min Li
author_facet	Xin Du Yuanjun Zhu Yan Song Tianming Li Tao Luo Gang Sun Chongguang Yang Cuiming Cao Yuan Lu Min Li
author_sort	Xin Du
title	Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
title_short	Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
title_full	Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
title_fullStr	Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
title_full_unstemmed	Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.
title_sort	molecular analysis of staphylococcus epidermidis strains isolated from community and hospital environments in china.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/158effb4403947b5bd7094ca1677bfce
work_keys_str_mv	AT xindu molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT yuanjunzhu molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT yansong molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT tianmingli molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT taoluo molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT gangsun molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT chongguangyang molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT cuimingcao molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT yuanlu molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina AT minli molecularanalysisofstaphylococcusepidermidisstrainsisolatedfromcommunityandhospitalenvironmentsinchina
_version_	1718422955822678016

Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China.

Ejemplares similares