Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course

Industrial Biotechnology and Applied Microbiology is an optional 128h-course for Chemistry and Biology students at the Faculty of Sciences, University of Buenos Aires, Argentina. This course is usually attended by 25 students, working in teams of two. The curriculum, with 8 lab exercises, includes a...

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Autores principales:	L. Raiger Iustman, N. I. López, S. M. Ruzal, D. L. Vullo
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/159335b6203a4d64850046b6ee165614
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spelling	oai:doaj.org-article:159335b6203a4d64850046b6ee1656142021-11-15T15:03:51ZBioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course10.1128/jmbe.v14i1.5581935-78851935-7877https://doaj.org/article/159335b6203a4d64850046b6ee1656142013-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v14i1.558https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Industrial Biotechnology and Applied Microbiology is an optional 128h-course for Chemistry and Biology students at the Faculty of Sciences, University of Buenos Aires, Argentina. This course is usually attended by 25 students, working in teams of two. The curriculum, with 8 lab exercises, includes an oil bioremediation practice covering an insight of bioremediation processes: the influence of pollutants on autochthonous microbiota, biodegrader isolation and biosurfactant production for bioavailability understanding. The experimental steps are: (A) evaluation of microbial tolerance to pollutants by constructing pristine soil microcosms contaminated with diesel or xylene and (B) isolation of degraders and biosurfactant production analysis. To check microbial tolerance, microcosms are incubated during one week at 25-28ºC. Samples are collected at 0, 4 and every 48 h for CFU/g soil testing. An initial decrease of total CFU/g related to toxicity is noticed. At the end of the experiment, a recovery of the CFU number is observed, evidencing enrichment in biodegraders. Some colonies from the CFU counting plates are streaked in M9-agar with diesel as sole carbon source. After a week, isolates are inoculated on M9-Broth supplemented with diesel to induce biosurfactant production. Surface tension and Emulsification Index are measured in culture supernatants to visualize tensioactive effect of bacterial products. Besides the improvement in the good microbiological practices, the students show enthusiasm in different aspects, depending on their own interests. While biology students explore and learn new concepts on solubility, emulsions and bioavailability, chemistry students show curiosity in bacterial behavior and manipulation of microorganisms for environmental benefits.L. Raiger IustmanN. I. LópezS. M. RuzalD. L. VulloAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 14, Iss 1, Pp 131-134 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 L. Raiger Iustman N. I. López S. M. Ruzal D. L. Vullo Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
description	Industrial Biotechnology and Applied Microbiology is an optional 128h-course for Chemistry and Biology students at the Faculty of Sciences, University of Buenos Aires, Argentina. This course is usually attended by 25 students, working in teams of two. The curriculum, with 8 lab exercises, includes an oil bioremediation practice covering an insight of bioremediation processes: the influence of pollutants on autochthonous microbiota, biodegrader isolation and biosurfactant production for bioavailability understanding. The experimental steps are: (A) evaluation of microbial tolerance to pollutants by constructing pristine soil microcosms contaminated with diesel or xylene and (B) isolation of degraders and biosurfactant production analysis. To check microbial tolerance, microcosms are incubated during one week at 25-28ºC. Samples are collected at 0, 4 and every 48 h for CFU/g soil testing. An initial decrease of total CFU/g related to toxicity is noticed. At the end of the experiment, a recovery of the CFU number is observed, evidencing enrichment in biodegraders. Some colonies from the CFU counting plates are streaked in M9-agar with diesel as sole carbon source. After a week, isolates are inoculated on M9-Broth supplemented with diesel to induce biosurfactant production. Surface tension and Emulsification Index are measured in culture supernatants to visualize tensioactive effect of bacterial products. Besides the improvement in the good microbiological practices, the students show enthusiasm in different aspects, depending on their own interests. While biology students explore and learn new concepts on solubility, emulsions and bioavailability, chemistry students show curiosity in bacterial behavior and manipulation of microorganisms for environmental benefits.
format	article
author	L. Raiger Iustman N. I. López S. M. Ruzal D. L. Vullo
author_facet	L. Raiger Iustman N. I. López S. M. Ruzal D. L. Vullo
author_sort	L. Raiger Iustman
title	Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
title_short	Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
title_full	Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
title_fullStr	Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
title_full_unstemmed	Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course
title_sort	bioremediation approaches in a laboratory activity for the industrial biotechnology and applied microbiology (ibam) course
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/159335b6203a4d64850046b6ee165614
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Bioremediation Approaches in a Laboratory Activity for the Industrial Biotechnology and Applied Microbiology (IBAM) Course

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