Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.

<h4>Background</h4>Sudden death syndrome (SDS) caused by the ascomycete fungus, Fusarium virguliforme, exhibits root necrosis and leaf scorch or foliar SDS. The pathogen has never been identified from the above ground diseased foliar tissues. Foliar SDS is believed to be caused by host s...

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Autores principales:	Nilwala S Abeysekara, Madan K Bhattacharyya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/15992507557c46f1ac778ba6f1d7f27d
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spelling	oai:doaj.org-article:15992507557c46f1ac778ba6f1d7f27d2021-11-18T08:18:36ZAnalyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.1932-620310.1371/journal.pone.0093667https://doaj.org/article/15992507557c46f1ac778ba6f1d7f27d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24845418/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Sudden death syndrome (SDS) caused by the ascomycete fungus, Fusarium virguliforme, exhibits root necrosis and leaf scorch or foliar SDS. The pathogen has never been identified from the above ground diseased foliar tissues. Foliar SDS is believed to be caused by host selective toxins, including FvTox1, secreted by the fungus. This study investigated if the xylem sap of F. virguliforme-infected soybean plants contains secreted F. virguliforme-proteins, some of which could cause foliar SDS development.<h4>Results</h4>Xylem sap samples were collected from five biological replications of F. virguliforme-infected and uninfected soybean plants under controlled conditions. We identified five F. virguliforme proteins from the xylem sap of the F. virguliforme-infected soybean plants by conducting LC-ESI-MS/MS analysis. These five proteins were also present in the excreted proteome of the pathogen in culture filtrates. One of these proteins showed high sequence identity to cerato-platanin, a phytotoxin produced by Ceratocystis fimbriata f. sp. platani to cause canker stain disease in the plane tree. Of over 500 soybean proteins identified in this study, 112 were present in at least 80% of the sap samples collected from F. virguliforme-infected and -uninfected control plants. We have identified four soybean defense proteins from the xylem sap of F. virguliforme-infected soybean plants. The data have been deposited to the ProteomeXchange with identifier PXD000873.<h4>Conclusion</h4>This study confirms that a few F. virguliforme proteins travel through the xylem, some of which could be involved in foliar SDS development. We have identified five candidate proteinaceous toxins, one of which showed high similarity to a previously characterized phytotoxin. We have also shown the presence of four soybean defense proteins in the xylem sap of F. virguliforme-infected soybean plants. This study laid the foundation for studying the molecular basis of foliar SDS development in soybean and possible defense mechanisms that may be involved in conferring immunity against F. virguliforme and other soybean pathogens.Nilwala S AbeysekaraMadan K BhattacharyyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e93667 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nilwala S Abeysekara Madan K Bhattacharyya Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
description	<h4>Background</h4>Sudden death syndrome (SDS) caused by the ascomycete fungus, Fusarium virguliforme, exhibits root necrosis and leaf scorch or foliar SDS. The pathogen has never been identified from the above ground diseased foliar tissues. Foliar SDS is believed to be caused by host selective toxins, including FvTox1, secreted by the fungus. This study investigated if the xylem sap of F. virguliforme-infected soybean plants contains secreted F. virguliforme-proteins, some of which could cause foliar SDS development.<h4>Results</h4>Xylem sap samples were collected from five biological replications of F. virguliforme-infected and uninfected soybean plants under controlled conditions. We identified five F. virguliforme proteins from the xylem sap of the F. virguliforme-infected soybean plants by conducting LC-ESI-MS/MS analysis. These five proteins were also present in the excreted proteome of the pathogen in culture filtrates. One of these proteins showed high sequence identity to cerato-platanin, a phytotoxin produced by Ceratocystis fimbriata f. sp. platani to cause canker stain disease in the plane tree. Of over 500 soybean proteins identified in this study, 112 were present in at least 80% of the sap samples collected from F. virguliforme-infected and -uninfected control plants. We have identified four soybean defense proteins from the xylem sap of F. virguliforme-infected soybean plants. The data have been deposited to the ProteomeXchange with identifier PXD000873.<h4>Conclusion</h4>This study confirms that a few F. virguliforme proteins travel through the xylem, some of which could be involved in foliar SDS development. We have identified five candidate proteinaceous toxins, one of which showed high similarity to a previously characterized phytotoxin. We have also shown the presence of four soybean defense proteins in the xylem sap of F. virguliforme-infected soybean plants. This study laid the foundation for studying the molecular basis of foliar SDS development in soybean and possible defense mechanisms that may be involved in conferring immunity against F. virguliforme and other soybean pathogens.
format	article
author	Nilwala S Abeysekara Madan K Bhattacharyya
author_facet	Nilwala S Abeysekara Madan K Bhattacharyya
author_sort	Nilwala S Abeysekara
title	Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
title_short	Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
title_full	Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
title_fullStr	Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
title_full_unstemmed	Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.
title_sort	analyses of the xylem sap proteomes identified candidate fusarium virguliforme proteinacious toxins.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/15992507557c46f1ac778ba6f1d7f27d
work_keys_str_mv	AT nilwalasabeysekara analysesofthexylemsapproteomesidentifiedcandidatefusariumvirguliformeproteinacioustoxins AT madankbhattacharyya analysesofthexylemsapproteomesidentifiedcandidatefusariumvirguliformeproteinacioustoxins
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Analyses of the xylem sap proteomes identified candidate Fusarium virguliforme proteinacious toxins.

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