The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination

Abstract Background Myelination is a highly regulated process in the vertebrate central nervous system (CNS) whereby oligodendrocytes wrap axons with multiple layers of insulating myelin in order to allow rapid electrical conduction. Establishing the proper pattern of myelin in neural circuits requi...

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Autores principales:	Curtis M. Hay, Stacey Jackson, Stanislaw Mitew, Daniel J. Scott, Matthias Koenning, AeSoon L. Bensen, Helena Bujalka, Trevor J. Kilpatrick, Ben Emery
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	CNS myelination Oligodendrocyte Glia Myelin sheath Axoglial interactions Gpr62 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/159a6260f6b64efdab878264bb9636be
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spelling	oai:doaj.org-article:159a6260f6b64efdab878264bb9636be2021-12-05T12:04:41ZThe oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination10.1186/s13064-021-00156-y1749-8104https://doaj.org/article/159a6260f6b64efdab878264bb9636be2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13064-021-00156-yhttps://doaj.org/toc/1749-8104Abstract Background Myelination is a highly regulated process in the vertebrate central nervous system (CNS) whereby oligodendrocytes wrap axons with multiple layers of insulating myelin in order to allow rapid electrical conduction. Establishing the proper pattern of myelin in neural circuits requires communicative axo-glial interactions, however, the molecular interactions that occur between oligodendrocytes and axons during developmental myelination and myelin maintenance remain to be fully elucidated. Our previous work identified G protein-coupled receptor 62 (Gpr62), an uncharacterized orphan g-protein coupled receptor, as being selectively expressed by mature oligodendrocytes within the CNS, suggesting a potential role in myelination or axoglial interactions. However, no studies to date have assessed the functional requirement for Gpr62 in oligodendrocyte development or CNS myelination. Methods To address this, we generated a knockout mouse strain lacking the Gpr62 gene. We assessed CNS myelination during both postnatal development and adulthood using immunohistochemistry, electron microscopy and western blot. In addition, we utilized AAV-mediated expression of a tagged Gpr62 in oligodendrocytes to determine the subcellular localization of the protein in vivo. Results We find that virally expressed Gpr62 protein is selectively expressed on the adaxonal myelin layer, suggestive of a potential role for Gpr62 in axo-myelinic signaling. Nevertheless, Gpr62 knockout mice display normal oligodendrocyte numbers and apparently normal myelination within the CNS during both postnatal development and adulthood. Conclusions We conclude that in spite of being well-placed to mediate neuronal-oligodendrocyte communications, Gpr62 is overall dispensable for CNS myelination.Curtis M. HayStacey JacksonStanislaw MitewDaniel J. ScottMatthias KoenningAeSoon L. BensenHelena BujalkaTrevor J. KilpatrickBen EmeryBMCarticleCNS myelinationOligodendrocyteGliaMyelin sheathAxoglial interactionsGpr62Neurology. Diseases of the nervous systemRC346-429ENNeural Development, Vol 16, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CNS myelination Oligodendrocyte Glia Myelin sheath Axoglial interactions Gpr62 Neurology. Diseases of the nervous system RC346-429
spellingShingle	CNS myelination Oligodendrocyte Glia Myelin sheath Axoglial interactions Gpr62 Neurology. Diseases of the nervous system RC346-429 Curtis M. Hay Stacey Jackson Stanislaw Mitew Daniel J. Scott Matthias Koenning AeSoon L. Bensen Helena Bujalka Trevor J. Kilpatrick Ben Emery The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
description	Abstract Background Myelination is a highly regulated process in the vertebrate central nervous system (CNS) whereby oligodendrocytes wrap axons with multiple layers of insulating myelin in order to allow rapid electrical conduction. Establishing the proper pattern of myelin in neural circuits requires communicative axo-glial interactions, however, the molecular interactions that occur between oligodendrocytes and axons during developmental myelination and myelin maintenance remain to be fully elucidated. Our previous work identified G protein-coupled receptor 62 (Gpr62), an uncharacterized orphan g-protein coupled receptor, as being selectively expressed by mature oligodendrocytes within the CNS, suggesting a potential role in myelination or axoglial interactions. However, no studies to date have assessed the functional requirement for Gpr62 in oligodendrocyte development or CNS myelination. Methods To address this, we generated a knockout mouse strain lacking the Gpr62 gene. We assessed CNS myelination during both postnatal development and adulthood using immunohistochemistry, electron microscopy and western blot. In addition, we utilized AAV-mediated expression of a tagged Gpr62 in oligodendrocytes to determine the subcellular localization of the protein in vivo. Results We find that virally expressed Gpr62 protein is selectively expressed on the adaxonal myelin layer, suggestive of a potential role for Gpr62 in axo-myelinic signaling. Nevertheless, Gpr62 knockout mice display normal oligodendrocyte numbers and apparently normal myelination within the CNS during both postnatal development and adulthood. Conclusions We conclude that in spite of being well-placed to mediate neuronal-oligodendrocyte communications, Gpr62 is overall dispensable for CNS myelination.
format	article
author	Curtis M. Hay Stacey Jackson Stanislaw Mitew Daniel J. Scott Matthias Koenning AeSoon L. Bensen Helena Bujalka Trevor J. Kilpatrick Ben Emery
author_facet	Curtis M. Hay Stacey Jackson Stanislaw Mitew Daniel J. Scott Matthias Koenning AeSoon L. Bensen Helena Bujalka Trevor J. Kilpatrick Ben Emery
author_sort	Curtis M. Hay
title	The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
title_short	The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
title_full	The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
title_fullStr	The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
title_full_unstemmed	The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination
title_sort	oligodendrocyte-enriched orphan g protein-coupled receptor gpr62 is dispensable for central nervous system myelination
publisher	BMC
publishDate	2021
url	https://doaj.org/article/159a6260f6b64efdab878264bb9636be
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The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination

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