Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems

Abstract Trehalose is a non‐reducing disaccharide widely distributed in nature. The trehalose biosynthetic intermediate, trehalose 6‐phosphate (Tre6P) is an essential regulatory and signaling molecule involved in both regulation of carbon metabolism and photosynthesis. To investigate the effect of a...

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Autores principales:	Casey Gabriel, Jean‐Jacque Fernhout, Franziska Fichtner, Regina Feil, John E. Lunn, Jens Kossmann, James R. Lloyd, Christell van derVyver
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	otsA otsB sugarcane (Saccharum spp) trehalose trehalose 6‐phosphate trehalose‐6‐phosphate phosphatase Botany QK1-989
Acceso en línea:	https://doaj.org/article/159bbccc56084e9d958c7f1833f19706
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spelling	oai:doaj.org-article:159bbccc56084e9d958c7f1833f197062021-11-29T07:25:55ZGenetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems2475-445510.1002/pld3.358https://doaj.org/article/159bbccc56084e9d958c7f1833f197062021-11-01T00:00:00Zhttps://doi.org/10.1002/pld3.358https://doaj.org/toc/2475-4455Abstract Trehalose is a non‐reducing disaccharide widely distributed in nature. The trehalose biosynthetic intermediate, trehalose 6‐phosphate (Tre6P) is an essential regulatory and signaling molecule involved in both regulation of carbon metabolism and photosynthesis. To investigate the effect of altered trehalose synthesis on sucrose accumulation in sugarcane (Saccharum spp. hybrid), we independently overexpressed the Escherichia coli otsA (trehalose‐6‐phosphate synthase; TPS) and otsB (trehalose‐6‐phosphate phosphatase; TPP) genes and additionally partially silenced native TPS expression. In mature cane, sucrose levels in the otsA transgenic plants were lowered, whereas sucrose levels in the otsB transgenic plants were increased. Partial silencing of TPS expression in sugarcane transformed with a TPS‐targeted microRNA recombinant construct was confirmed in leaf and mature internode tissue of transgenic plants. Most of the silencing transgenic lines accumulated trehalose at lower levels than the wild‐type (WT) plants. The immature stalk tissue of these transgenic lines had lower levels of glucose and fructose, whereas the mature internode tissue had lower sucrose and glucose levels, when compared with the WT. Furthermore, various minor metabolites and sugars were detected in the sugarcane plants, which mostly decreased as the stalk tissue of the cane matured. The results demonstrate that manipulation of Tre6P/trehalose metabolism has the potential to modify the profile of soluble sugars accumulated in sugarcane stems.Casey GabrielJean‐Jacque FernhoutFranziska FichtnerRegina FeilJohn E. LunnJens KossmannJames R. LloydChristell van derVyverWileyarticleotsAotsBsugarcane (Saccharum spp)trehalosetrehalose 6‐phosphatetrehalose‐6‐phosphate phosphataseBotanyQK1-989ENPlant Direct, Vol 5, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	otsA otsB sugarcane (Saccharum spp) trehalose trehalose 6‐phosphate trehalose‐6‐phosphate phosphatase Botany QK1-989
spellingShingle	otsA otsB sugarcane (Saccharum spp) trehalose trehalose 6‐phosphate trehalose‐6‐phosphate phosphatase Botany QK1-989 Casey Gabriel Jean‐Jacque Fernhout Franziska Fichtner Regina Feil John E. Lunn Jens Kossmann James R. Lloyd Christell van derVyver Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
description	Abstract Trehalose is a non‐reducing disaccharide widely distributed in nature. The trehalose biosynthetic intermediate, trehalose 6‐phosphate (Tre6P) is an essential regulatory and signaling molecule involved in both regulation of carbon metabolism and photosynthesis. To investigate the effect of altered trehalose synthesis on sucrose accumulation in sugarcane (Saccharum spp. hybrid), we independently overexpressed the Escherichia coli otsA (trehalose‐6‐phosphate synthase; TPS) and otsB (trehalose‐6‐phosphate phosphatase; TPP) genes and additionally partially silenced native TPS expression. In mature cane, sucrose levels in the otsA transgenic plants were lowered, whereas sucrose levels in the otsB transgenic plants were increased. Partial silencing of TPS expression in sugarcane transformed with a TPS‐targeted microRNA recombinant construct was confirmed in leaf and mature internode tissue of transgenic plants. Most of the silencing transgenic lines accumulated trehalose at lower levels than the wild‐type (WT) plants. The immature stalk tissue of these transgenic lines had lower levels of glucose and fructose, whereas the mature internode tissue had lower sucrose and glucose levels, when compared with the WT. Furthermore, various minor metabolites and sugars were detected in the sugarcane plants, which mostly decreased as the stalk tissue of the cane matured. The results demonstrate that manipulation of Tre6P/trehalose metabolism has the potential to modify the profile of soluble sugars accumulated in sugarcane stems.
format	article
author	Casey Gabriel Jean‐Jacque Fernhout Franziska Fichtner Regina Feil John E. Lunn Jens Kossmann James R. Lloyd Christell van derVyver
author_facet	Casey Gabriel Jean‐Jacque Fernhout Franziska Fichtner Regina Feil John E. Lunn Jens Kossmann James R. Lloyd Christell van derVyver
author_sort	Casey Gabriel
title	Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
title_short	Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
title_full	Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
title_fullStr	Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
title_full_unstemmed	Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
title_sort	genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/159bbccc56084e9d958c7f1833f19706
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Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems

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