Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids

Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids

Zhengguo Qiu,1 Qianhe Shen,2 Chao Jiang,3 Li Yao,4 Xiaopeng Sun,5 Jing Li,6 Chongzhen Duan,1 Rui Li,1 Xiuli Li,1 Subash CB Gopinath,7,8 Periasamy Anbu,9 Thangavel Lakshmipriya,8 Xu Li10 1Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang...

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Autores principales: Qiu Z, Shen Q, Jiang C, Yao L, Sun X, Li J, Duan C, Li R, Li X, Gopinath SCB, Anbu P, Lakshmipriya T
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2021
Materias:
amyloid-beta
biomarker
nanomaterial
aptamer
antibody
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/15a9c0fa73204dcca8484f8a8e3e4e35
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spelling oai:doaj.org-article:15a9c0fa73204dcca8484f8a8e3e4e352021-12-02T14:25:43ZAlzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids1178-2013https://doaj.org/article/15a9c0fa73204dcca8484f8a8e3e4e352021-03-01T00:00:00Zhttps://www.dovepress.com/alzheimerrsquos-disease-determination-by-a-dual-probe-on-gold-nanourch-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhengguo Qiu,1 Qianhe Shen,2 Chao Jiang,3 Li Yao,4 Xiaopeng Sun,5 Jing Li,6 Chongzhen Duan,1 Rui Li,1 Xiuli Li,1 Subash CB Gopinath,7,8 Periasamy Anbu,9 Thangavel Lakshmipriya,8 Xu Li10 1Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, 712000, People’s Republic of China; 2Department of Anesthesiology, Xi’an GemFlower Changqing Hospital, Xi’an, Shaanxi, 710200, People’s Republic of China; 3The Third Department of Neurology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 4Department of Neurology, The Hospital of Xidian Group, Xi’an, Shaanxi, 710077, People’s Republic of China; 5Department of Otolaryngology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 6Department of Anesthesiology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 7Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau, Perlis, 02600, Malaysia; 8Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Kangar, 01000, Malaysia; 9Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea; 10Department of Surgery and Anesthesiology, Xi’an Hospital of Traditional Chinese Medicine, Xi’an, Shaanxi, People’s Republic of ChinaCorrespondence: Xu Li Email qzg2020@sina.comBackground: Alzheimer’s disease (AD) is a neurodegenerative chronic disorder that causes dementia and problems in thinking, cognitive impairment and behavioral changes. Amyloid-beta (Aβ) is a peptide involved in AD progression, and a high level of Aβ is highly correlated with severe AD. Identifying and quantifying Aβ levels helps in the early treatment of AD and reduces the factors associated with AD.Materials and Methods: This research introduced a dual probe detection system involving aptamers and antibodies to identify Aβ. Aptamers and antibodies were attached to the gold (Au) urchin and hybrid on the carbon nanohorn-modified surface. The nanohorn was immobilized on the sensor surface by using an amine linker, and then a Au urchin dual probe was immobilized.Results: This dual probe-modified surface enhanced the current flow during Aβ detection compared with the surface with antibody as the probe. This dual probe interacted with higher numbers of Aβ peptides and reached the detection limit at 10 fM with R2=0.992. Furthermore, control experiments with nonimmune antibodies, complementary aptamer sequences and control proteins did not display the current responses, indicating the specific detection of Aβ.Conclusion: Aβ-spiked artificial cerebrospinal fluid showed a similar response to current changes, confirming the selective identification of Aβ.Keywords: amyloid-beta, biomarker, nanomaterial, aptamer, antibodyQiu ZShen QJiang CYao LSun XLi JDuan CLi RLi XGopinath SCBAnbu PLakshmipriya TLi XDove Medical Pressarticleamyloid-betabiomarkernanomaterialaptamerantibodyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 2311-2322 (2021)
institution DOAJ
collection DOAJ
language EN
topic amyloid-beta
biomarker
nanomaterial
aptamer
antibody
Medicine (General)
R5-920
spellingShingle amyloid-beta
biomarker
nanomaterial
aptamer
antibody
Medicine (General)
R5-920
Qiu Z
Shen Q
Jiang C
Yao L
Sun X
Li J
Duan C
Li R
Li X
Gopinath SCB
Anbu P
Lakshmipriya T
Li X
Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
description Zhengguo Qiu,1 Qianhe Shen,2 Chao Jiang,3 Li Yao,4 Xiaopeng Sun,5 Jing Li,6 Chongzhen Duan,1 Rui Li,1 Xiuli Li,1 Subash CB Gopinath,7,8 Periasamy Anbu,9 Thangavel Lakshmipriya,8 Xu Li10 1Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, 712000, People’s Republic of China; 2Department of Anesthesiology, Xi’an GemFlower Changqing Hospital, Xi’an, Shaanxi, 710200, People’s Republic of China; 3The Third Department of Neurology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 4Department of Neurology, The Hospital of Xidian Group, Xi’an, Shaanxi, 710077, People’s Republic of China; 5Department of Otolaryngology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 6Department of Anesthesiology, The Second Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, 710038, People’s Republic of China; 7Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau, Perlis, 02600, Malaysia; 8Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Kangar, 01000, Malaysia; 9Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea; 10Department of Surgery and Anesthesiology, Xi’an Hospital of Traditional Chinese Medicine, Xi’an, Shaanxi, People’s Republic of ChinaCorrespondence: Xu Li Email qzg2020@sina.comBackground: Alzheimer’s disease (AD) is a neurodegenerative chronic disorder that causes dementia and problems in thinking, cognitive impairment and behavioral changes. Amyloid-beta (Aβ) is a peptide involved in AD progression, and a high level of Aβ is highly correlated with severe AD. Identifying and quantifying Aβ levels helps in the early treatment of AD and reduces the factors associated with AD.Materials and Methods: This research introduced a dual probe detection system involving aptamers and antibodies to identify Aβ. Aptamers and antibodies were attached to the gold (Au) urchin and hybrid on the carbon nanohorn-modified surface. The nanohorn was immobilized on the sensor surface by using an amine linker, and then a Au urchin dual probe was immobilized.Results: This dual probe-modified surface enhanced the current flow during Aβ detection compared with the surface with antibody as the probe. This dual probe interacted with higher numbers of Aβ peptides and reached the detection limit at 10 fM with R2=0.992. Furthermore, control experiments with nonimmune antibodies, complementary aptamer sequences and control proteins did not display the current responses, indicating the specific detection of Aβ.Conclusion: Aβ-spiked artificial cerebrospinal fluid showed a similar response to current changes, confirming the selective identification of Aβ.Keywords: amyloid-beta, biomarker, nanomaterial, aptamer, antibody
format article
author Qiu Z
Shen Q
Jiang C
Yao L
Sun X
Li J
Duan C
Li R
Li X
Gopinath SCB
Anbu P
Lakshmipriya T
Li X
author_facet Qiu Z
Shen Q
Jiang C
Yao L
Sun X
Li J
Duan C
Li R
Li X
Gopinath SCB
Anbu P
Lakshmipriya T
Li X
author_sort Qiu Z
title Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
title_short Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
title_full Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
title_fullStr Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
title_full_unstemmed Alzheimer’s Disease Determination by a Dual Probe on Gold Nanourchins and Nanohorn Hybrids
title_sort alzheimer’s disease determination by a dual probe on gold nanourchins and nanohorn hybrids
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/15a9c0fa73204dcca8484f8a8e3e4e35
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