PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE

The oil palm industry in Colombia, the fifth world manufacter, generates lignocelulosic waste like the empty fruit bunch (EFB), which remains after removing the fruit of oil palm. Keeping in mind this characteristic, it can be used as substrate for cellulases production. In this study we evaluate th...

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Autores principales: Ibeth RODRÍGUEZ G., Yineth PIÑEROS C.
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Publicado: Universidad de Antioquia 2009
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spelling oai:doaj.org-article:15af822ac9af41e0b264b3e58b459a842021-11-19T04:21:06ZPRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE0121-40042145-2660https://doaj.org/article/15af822ac9af41e0b264b3e58b459a842009-02-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/606https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660The oil palm industry in Colombia, the fifth world manufacter, generates lignocelulosic waste like the empty fruit bunch (EFB), which remains after removing the fruit of oil palm. Keeping in mind this characteristic, it can be used as substrate for cellulases production. In this study we evaluate the productivity of cellulases through native crop of Trichoderma viride T12 using EFB. The wastes are subject to chemical (HNO3 0,5% y 1%) and biological treatments (previous cultivate with Pleurotus ostreatus during 10 and 20 days), to get its deslignification and improving the production of cellulases. The evaluation of the activities of total cellulase (FPase) and endoglucanase (CMCase) was made after 8 days of cultivate by fermentation in solid state, using pretreated wastes as substrate. It was supplemented with two inorganic nitrogen sources (NH4H2PO4 y NaNO3). The major celulolitic activities were found in wastes treated with precultive of Pleurotus ostreatus and NaNO3 as nitrogen source, with values of 0,374 U/mL of FPase and 0,776 U/mL of CMCase. All activities were expressed in micromole of glucose units (U) of substrate hydrolyzed in 1 min. Ibeth RODRÍGUEZ G.Yineth PIÑEROS C.Universidad de AntioquiaarticleTrichoderma sp.ligninecellulosecellulasespalm oil wastes.Food processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 14, Iss 2 (2009)
institution DOAJ
collection DOAJ
language EN
topic Trichoderma sp.
lignine
cellulose
cellulases
palm oil wastes.
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
spellingShingle Trichoderma sp.
lignine
cellulose
cellulases
palm oil wastes.
Food processing and manufacture
TP368-456
Pharmaceutical industry
HD9665-9675
Ibeth RODRÍGUEZ G.
Yineth PIÑEROS C.
PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
description The oil palm industry in Colombia, the fifth world manufacter, generates lignocelulosic waste like the empty fruit bunch (EFB), which remains after removing the fruit of oil palm. Keeping in mind this characteristic, it can be used as substrate for cellulases production. In this study we evaluate the productivity of cellulases through native crop of Trichoderma viride T12 using EFB. The wastes are subject to chemical (HNO3 0,5% y 1%) and biological treatments (previous cultivate with Pleurotus ostreatus during 10 and 20 days), to get its deslignification and improving the production of cellulases. The evaluation of the activities of total cellulase (FPase) and endoglucanase (CMCase) was made after 8 days of cultivate by fermentation in solid state, using pretreated wastes as substrate. It was supplemented with two inorganic nitrogen sources (NH4H2PO4 y NaNO3). The major celulolitic activities were found in wastes treated with precultive of Pleurotus ostreatus and NaNO3 as nitrogen source, with values of 0,374 U/mL of FPase and 0,776 U/mL of CMCase. All activities were expressed in micromole of glucose units (U) of substrate hydrolyzed in 1 min.
format article
author Ibeth RODRÍGUEZ G.
Yineth PIÑEROS C.
author_facet Ibeth RODRÍGUEZ G.
Yineth PIÑEROS C.
author_sort Ibeth RODRÍGUEZ G.
title PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
title_short PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
title_full PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
title_fullStr PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
title_full_unstemmed PRODUCTION OF ENZYMATIC COMPLEX IN SOLID STATE FERMENTATION BY <I>Trichoderma sp</I>. USING PALMOIL EMPTY FRUIT PALM OIL BUNCH (EFB) AS SUBSTRATE
title_sort production of enzymatic complex in solid state fermentation by <i>trichoderma sp</i>. using palmoil empty fruit palm oil bunch (efb) as substrate
publisher Universidad de Antioquia
publishDate 2009
url https://doaj.org/article/15af822ac9af41e0b264b3e58b459a84
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